Transcriptome analysis and functional identification of GmMYB46 in soybean seedlings under salt stress

Salinity is one of the major abiotic stress that limits crop growth and productivity. We investigated the transcriptomes of salt-treated soybean seedlings versus a control using RNA-seq to better understand the molecular mechanisms of the soybean (Glycine max L.) response to salt stress. Transcripto...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Xun Liu, Xinxia Yang, Bin Zhang
Formato:	article
Lenguaje:	EN
Publicado:	PeerJ Inc. 2021
Materias:	Soybean Transcriptome analysis Salt stress Differentially expressed genes GmMYB46 Medicine R
Acceso en línea:	https://doaj.org/article/f116736375fc4e5fb52502adfe1b35ce
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f116736375fc4e5fb52502adfe1b35ce
record_format	dspace
spelling	oai:doaj.org-article:f116736375fc4e5fb52502adfe1b35ce2021-11-13T15:05:28ZTranscriptome analysis and functional identification of GmMYB46 in soybean seedlings under salt stress10.7717/peerj.124922167-8359https://doaj.org/article/f116736375fc4e5fb52502adfe1b35ce2021-11-01T00:00:00Zhttps://peerj.com/articles/12492.pdfhttps://peerj.com/articles/12492/https://doaj.org/toc/2167-8359Salinity is one of the major abiotic stress that limits crop growth and productivity. We investigated the transcriptomes of salt-treated soybean seedlings versus a control using RNA-seq to better understand the molecular mechanisms of the soybean (Glycine max L.) response to salt stress. Transcriptome analysis revealed 1,235 differentially expressed genes (DEGs) under salt stress. Several important pathways and key candidate genes were identified by KEGG enrichment. A total of 116 differentially expressed transcription factors (TFs) were identified, and 17 TFs were found to belong to MYB families. Phylogenetic analysis revealed that these TFs may be involved in salt stress adaptation. Further analysis revealed that GmMYB46 was up-regulated by salt and mannitol and was localized in the nucleus. The salt tolerance of transgenic Arabidopsis overexpressing GmMYB46 was significantly enhanced compared to wild-type (WT). GmMYB46 activates the expression of salt stress response genes (P5CS1, SOD, POD, NCED3) in Arabidopsis under salt stress, indicating that the GmMYB46 protein mediates the salt stress response through complex regulatory mechanisms. This study provides information with which to better understand the molecular mechanism of salt tolerance in soybeans and to genetically improve the crop.Xun LiuXinxia YangBin ZhangPeerJ Inc.articleSoybeanTranscriptome analysisSalt stressDifferentially expressed genesGmMYB46MedicineRENPeerJ, Vol 9, p e12492 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Soybean Transcriptome analysis Salt stress Differentially expressed genes GmMYB46 Medicine R
spellingShingle	Soybean Transcriptome analysis Salt stress Differentially expressed genes GmMYB46 Medicine R Xun Liu Xinxia Yang Bin Zhang Transcriptome analysis and functional identification of GmMYB46 in soybean seedlings under salt stress
description	Salinity is one of the major abiotic stress that limits crop growth and productivity. We investigated the transcriptomes of salt-treated soybean seedlings versus a control using RNA-seq to better understand the molecular mechanisms of the soybean (Glycine max L.) response to salt stress. Transcriptome analysis revealed 1,235 differentially expressed genes (DEGs) under salt stress. Several important pathways and key candidate genes were identified by KEGG enrichment. A total of 116 differentially expressed transcription factors (TFs) were identified, and 17 TFs were found to belong to MYB families. Phylogenetic analysis revealed that these TFs may be involved in salt stress adaptation. Further analysis revealed that GmMYB46 was up-regulated by salt and mannitol and was localized in the nucleus. The salt tolerance of transgenic Arabidopsis overexpressing GmMYB46 was significantly enhanced compared to wild-type (WT). GmMYB46 activates the expression of salt stress response genes (P5CS1, SOD, POD, NCED3) in Arabidopsis under salt stress, indicating that the GmMYB46 protein mediates the salt stress response through complex regulatory mechanisms. This study provides information with which to better understand the molecular mechanism of salt tolerance in soybeans and to genetically improve the crop.
format	article
author	Xun Liu Xinxia Yang Bin Zhang
author_facet	Xun Liu Xinxia Yang Bin Zhang
author_sort	Xun Liu
title	Transcriptome analysis and functional identification of GmMYB46 in soybean seedlings under salt stress
title_short	Transcriptome analysis and functional identification of GmMYB46 in soybean seedlings under salt stress
title_full	Transcriptome analysis and functional identification of GmMYB46 in soybean seedlings under salt stress
title_fullStr	Transcriptome analysis and functional identification of GmMYB46 in soybean seedlings under salt stress
title_full_unstemmed	Transcriptome analysis and functional identification of GmMYB46 in soybean seedlings under salt stress
title_sort	transcriptome analysis and functional identification of gmmyb46 in soybean seedlings under salt stress
publisher	PeerJ Inc.
publishDate	2021
url	https://doaj.org/article/f116736375fc4e5fb52502adfe1b35ce
work_keys_str_mv	AT xunliu transcriptomeanalysisandfunctionalidentificationofgmmyb46insoybeanseedlingsundersaltstress AT xinxiayang transcriptomeanalysisandfunctionalidentificationofgmmyb46insoybeanseedlingsundersaltstress AT binzhang transcriptomeanalysisandfunctionalidentificationofgmmyb46insoybeanseedlingsundersaltstress
_version_	1718430290158813184

Transcriptome analysis and functional identification of GmMYB46 in soybean seedlings under salt stress

Ejemplares similares