The 5′ Untranslated Region of the <italic toggle="yes">EFG1</italic> Transcript Promotes Its Translation To Regulate Hyphal Morphogenesis in <italic toggle="yes">Candida albicans</italic>

ABSTRACT Extensive 5′ untranslated regions (UTR) are a hallmark of transcripts determining hyphal morphogenesis in Candida albicans. The major transcripts of the EFG1 gene, which are responsible for cellular morphogenesis and metabolism, contain a 5′ UTR of up to 1,170 nucleotides (nt). Deletion ana...

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Autores principales:	Prashant R. Desai, Klaus Lengeler, Mario Kapitan, Silas Matthias Janßen, Paula Alepuz, Ilse D. Jacobsen, Joachim F. Ernst
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2018
Materias:	5′ UTR Candida albicans EFG1 filamentation hyphal morphogenesis posttranscriptional regulation Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/f117cc8e47d443b4b75f19d70c67bdbb
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spelling	oai:doaj.org-article:f117cc8e47d443b4b75f19d70c67bdbb2021-11-15T15:25:50ZThe 5′ Untranslated Region of the <italic toggle="yes">EFG1</italic> Transcript Promotes Its Translation To Regulate Hyphal Morphogenesis in <italic toggle="yes">Candida albicans</italic>10.1128/mSphere.00280-182379-5042https://doaj.org/article/f117cc8e47d443b4b75f19d70c67bdbb2018-08-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00280-18https://doaj.org/toc/2379-5042ABSTRACT Extensive 5′ untranslated regions (UTR) are a hallmark of transcripts determining hyphal morphogenesis in Candida albicans. The major transcripts of the EFG1 gene, which are responsible for cellular morphogenesis and metabolism, contain a 5′ UTR of up to 1,170 nucleotides (nt). Deletion analyses of the 5′ UTR revealed a 218-nt sequence that is required for production of the Efg1 protein and its functions in filamentation, without lowering the level and integrity of the EFG1 transcript. Polysomal analyses revealed that the 218-nt 5′ UTR sequence is required for efficient translation of the Efg1 protein. Replacement of the EFG1 open reading frame (ORF) by the heterologous reporter gene CaCBGluc confirmed the positive regulatory importance of the identified 5′ UTR sequence. In contrast to other reported transcripts containing extensive 5′ UTR sequences, these results indicate the positive translational function of the 5′ UTR sequence in the EFG1 transcript, which is observed in the context of the native EFG1 promoter. It is proposed that the 5′ UTR recruits regulatory factors, possibly during emergence of the native transcript, which aid in translation of the EFG1 transcript. IMPORTANCE Many of the virulence traits that make Candida albicans an important human fungal pathogen are regulated on a transcriptional level. Here, we report an important regulatory contribution of translation, which is exerted by the extensive 5′ untranslated regulatory sequence (5′ UTR) of the transcript for the protein Efg1, which determines growth, metabolism, and filamentation in the fungus. The presence of the 5′ UTR is required for efficient translation of Efg1, to promote filamentation. Because transcripts for many relevant regulators contain extensive 5′ UTR sequences, it appears that the virulence of C. albicans depends on the combination of transcriptional and translational regulatory mechanisms.Prashant R. DesaiKlaus LengelerMario KapitanSilas Matthias JanßenPaula AlepuzIlse D. JacobsenJoachim F. ErnstAmerican Society for Microbiologyarticle5′ UTRCandida albicansEFG1filamentationhyphal morphogenesisposttranscriptional regulationMicrobiologyQR1-502ENmSphere, Vol 3, Iss 4 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	5′ UTR Candida albicans EFG1 filamentation hyphal morphogenesis posttranscriptional regulation Microbiology QR1-502
spellingShingle	5′ UTR Candida albicans EFG1 filamentation hyphal morphogenesis posttranscriptional regulation Microbiology QR1-502 Prashant R. Desai Klaus Lengeler Mario Kapitan Silas Matthias Janßen Paula Alepuz Ilse D. Jacobsen Joachim F. Ernst The 5′ Untranslated Region of the <italic toggle="yes">EFG1</italic> Transcript Promotes Its Translation To Regulate Hyphal Morphogenesis in <italic toggle="yes">Candida albicans</italic>
description	ABSTRACT Extensive 5′ untranslated regions (UTR) are a hallmark of transcripts determining hyphal morphogenesis in Candida albicans. The major transcripts of the EFG1 gene, which are responsible for cellular morphogenesis and metabolism, contain a 5′ UTR of up to 1,170 nucleotides (nt). Deletion analyses of the 5′ UTR revealed a 218-nt sequence that is required for production of the Efg1 protein and its functions in filamentation, without lowering the level and integrity of the EFG1 transcript. Polysomal analyses revealed that the 218-nt 5′ UTR sequence is required for efficient translation of the Efg1 protein. Replacement of the EFG1 open reading frame (ORF) by the heterologous reporter gene CaCBGluc confirmed the positive regulatory importance of the identified 5′ UTR sequence. In contrast to other reported transcripts containing extensive 5′ UTR sequences, these results indicate the positive translational function of the 5′ UTR sequence in the EFG1 transcript, which is observed in the context of the native EFG1 promoter. It is proposed that the 5′ UTR recruits regulatory factors, possibly during emergence of the native transcript, which aid in translation of the EFG1 transcript. IMPORTANCE Many of the virulence traits that make Candida albicans an important human fungal pathogen are regulated on a transcriptional level. Here, we report an important regulatory contribution of translation, which is exerted by the extensive 5′ untranslated regulatory sequence (5′ UTR) of the transcript for the protein Efg1, which determines growth, metabolism, and filamentation in the fungus. The presence of the 5′ UTR is required for efficient translation of Efg1, to promote filamentation. Because transcripts for many relevant regulators contain extensive 5′ UTR sequences, it appears that the virulence of C. albicans depends on the combination of transcriptional and translational regulatory mechanisms.
format	article
author	Prashant R. Desai Klaus Lengeler Mario Kapitan Silas Matthias Janßen Paula Alepuz Ilse D. Jacobsen Joachim F. Ernst
author_facet	Prashant R. Desai Klaus Lengeler Mario Kapitan Silas Matthias Janßen Paula Alepuz Ilse D. Jacobsen Joachim F. Ernst
author_sort	Prashant R. Desai
title	The 5′ Untranslated Region of the <italic toggle="yes">EFG1</italic> Transcript Promotes Its Translation To Regulate Hyphal Morphogenesis in <italic toggle="yes">Candida albicans</italic>
title_short	The 5′ Untranslated Region of the <italic toggle="yes">EFG1</italic> Transcript Promotes Its Translation To Regulate Hyphal Morphogenesis in <italic toggle="yes">Candida albicans</italic>
title_full	The 5′ Untranslated Region of the <italic toggle="yes">EFG1</italic> Transcript Promotes Its Translation To Regulate Hyphal Morphogenesis in <italic toggle="yes">Candida albicans</italic>
title_fullStr	The 5′ Untranslated Region of the <italic toggle="yes">EFG1</italic> Transcript Promotes Its Translation To Regulate Hyphal Morphogenesis in <italic toggle="yes">Candida albicans</italic>
title_full_unstemmed	The 5′ Untranslated Region of the <italic toggle="yes">EFG1</italic> Transcript Promotes Its Translation To Regulate Hyphal Morphogenesis in <italic toggle="yes">Candida albicans</italic>
title_sort	5′ untranslated region of the <italic toggle="yes">efg1</italic> transcript promotes its translation to regulate hyphal morphogenesis in <italic toggle="yes">candida albicans</italic>
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/f117cc8e47d443b4b75f19d70c67bdbb
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The 5′ Untranslated Region of the <italic toggle="yes">EFG1</italic> Transcript Promotes Its Translation To Regulate Hyphal Morphogenesis in <italic toggle="yes">Candida albicans</italic>

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