Investigating the Reciprocal Interrelationships among the Ruminal Microbiota, Metabolome, and Mastitis in Early Lactating Holstein Dairy Cows

Mastitis in dairy cow significantly affects animal performance, ultimately reducing profitability. The reciprocal interrelationships among ruminal microbiota, metabolome, and mastitis combining early inflammatory factors (serum proinflammatory cytokines) in lactating dairy cows has not been explored...

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Autores principales:	Shih-Te Chuang, Kuan-Yi Li, Po-Wen Tu, Shang-Tse Ho, Cheng-Chih Hsu, Jui-Chun Hsieh, Ming-Ju Chen
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	ruminal microbiota metabolome mastitis Holstein dairy cows Veterinary medicine SF600-1100 Zoology QL1-991
Acceso en línea:	https://doaj.org/article/f119fcf475e2496090c3a56af790d1be
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spelling	oai:doaj.org-article:f119fcf475e2496090c3a56af790d1be2021-11-25T16:15:37ZInvestigating the Reciprocal Interrelationships among the Ruminal Microbiota, Metabolome, and Mastitis in Early Lactating Holstein Dairy Cows10.3390/ani111131082076-2615https://doaj.org/article/f119fcf475e2496090c3a56af790d1be2021-10-01T00:00:00Zhttps://www.mdpi.com/2076-2615/11/11/3108https://doaj.org/toc/2076-2615Mastitis in dairy cow significantly affects animal performance, ultimately reducing profitability. The reciprocal interrelationships among ruminal microbiota, metabolome, and mastitis combining early inflammatory factors (serum proinflammatory cytokines) in lactating dairy cows has not been explored, thus, this study evaluated these reciprocal interrelationships in early lactating Holstein dairy cows to identify potential microbial biomarkers and their relationship with ruminal metabolites. The ruminal fluid was sampled from 8 healthy and 8 mastitis cows for the microbiota and metabolite analyses. The critical ruminal microbial biomarkers and metabolites related to somatic cell counts (SCC) and serum proinflammatory cytokines were identified by the linear discriminant analysis effect size (LEfSe) algorithm and Spearman’s correlation analysis, respectively. The SCC level and proinflammatory cytokines positively correlated with <i>Sharpea</i> and negatively correlated with <i>Ruminococcaceae UCG-014</i>, <i>Ruminococcus flavefaciens</i>, and <i>Treponema saccharophilum</i>. Furthermore, the metabolites xanthurenic acid, and 1-(1H-benzo[d]imidazol-2-yl) ethan-1-ol positively correlated with microbial biomarkers of healthy cows, whereas, xanthine, pantothenic acid, and anacardic acid were negatively correlated with the microbial biomarkers of mastitis cows. In conclusion, <i>Ruminococcus flavefaciens</i> and <i>Treponema saccharophilum</i> are potential strains for improving the health of dairy cows. The current study provides a novel perspective to assist in targeting the ruminal microbiota with preventive/therapeutic strategies against inflammatory diseases in the future.Shih-Te ChuangKuan-Yi LiPo-Wen TuShang-Tse HoCheng-Chih HsuJui-Chun HsiehMing-Ju ChenMDPI AGarticleruminal microbiotametabolomemastitisHolstein dairy cowsVeterinary medicineSF600-1100ZoologyQL1-991ENAnimals, Vol 11, Iss 3108, p 3108 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	ruminal microbiota metabolome mastitis Holstein dairy cows Veterinary medicine SF600-1100 Zoology QL1-991
spellingShingle	ruminal microbiota metabolome mastitis Holstein dairy cows Veterinary medicine SF600-1100 Zoology QL1-991 Shih-Te Chuang Kuan-Yi Li Po-Wen Tu Shang-Tse Ho Cheng-Chih Hsu Jui-Chun Hsieh Ming-Ju Chen Investigating the Reciprocal Interrelationships among the Ruminal Microbiota, Metabolome, and Mastitis in Early Lactating Holstein Dairy Cows
description	Mastitis in dairy cow significantly affects animal performance, ultimately reducing profitability. The reciprocal interrelationships among ruminal microbiota, metabolome, and mastitis combining early inflammatory factors (serum proinflammatory cytokines) in lactating dairy cows has not been explored, thus, this study evaluated these reciprocal interrelationships in early lactating Holstein dairy cows to identify potential microbial biomarkers and their relationship with ruminal metabolites. The ruminal fluid was sampled from 8 healthy and 8 mastitis cows for the microbiota and metabolite analyses. The critical ruminal microbial biomarkers and metabolites related to somatic cell counts (SCC) and serum proinflammatory cytokines were identified by the linear discriminant analysis effect size (LEfSe) algorithm and Spearman’s correlation analysis, respectively. The SCC level and proinflammatory cytokines positively correlated with <i>Sharpea</i> and negatively correlated with <i>Ruminococcaceae UCG-014</i>, <i>Ruminococcus flavefaciens</i>, and <i>Treponema saccharophilum</i>. Furthermore, the metabolites xanthurenic acid, and 1-(1H-benzo[d]imidazol-2-yl) ethan-1-ol positively correlated with microbial biomarkers of healthy cows, whereas, xanthine, pantothenic acid, and anacardic acid were negatively correlated with the microbial biomarkers of mastitis cows. In conclusion, <i>Ruminococcus flavefaciens</i> and <i>Treponema saccharophilum</i> are potential strains for improving the health of dairy cows. The current study provides a novel perspective to assist in targeting the ruminal microbiota with preventive/therapeutic strategies against inflammatory diseases in the future.
format	article
author	Shih-Te Chuang Kuan-Yi Li Po-Wen Tu Shang-Tse Ho Cheng-Chih Hsu Jui-Chun Hsieh Ming-Ju Chen
author_facet	Shih-Te Chuang Kuan-Yi Li Po-Wen Tu Shang-Tse Ho Cheng-Chih Hsu Jui-Chun Hsieh Ming-Ju Chen
author_sort	Shih-Te Chuang
title	Investigating the Reciprocal Interrelationships among the Ruminal Microbiota, Metabolome, and Mastitis in Early Lactating Holstein Dairy Cows
title_short	Investigating the Reciprocal Interrelationships among the Ruminal Microbiota, Metabolome, and Mastitis in Early Lactating Holstein Dairy Cows
title_full	Investigating the Reciprocal Interrelationships among the Ruminal Microbiota, Metabolome, and Mastitis in Early Lactating Holstein Dairy Cows
title_fullStr	Investigating the Reciprocal Interrelationships among the Ruminal Microbiota, Metabolome, and Mastitis in Early Lactating Holstein Dairy Cows
title_full_unstemmed	Investigating the Reciprocal Interrelationships among the Ruminal Microbiota, Metabolome, and Mastitis in Early Lactating Holstein Dairy Cows
title_sort	investigating the reciprocal interrelationships among the ruminal microbiota, metabolome, and mastitis in early lactating holstein dairy cows
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/f119fcf475e2496090c3a56af790d1be
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Investigating the Reciprocal Interrelationships among the Ruminal Microbiota, Metabolome, and Mastitis in Early Lactating Holstein Dairy Cows

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