Proven and less studied hematopoietic and vasoactive growth factors in retinal capillary hemangioma

Pathogenesis of retinal capillary hemangioma has not been sufficiently studied at the present time. Therefore, the study of cytokine levels in biological fluids seems to be very relevant in order to increase knowledge about the mechanisms of the disease development and searching for targeted therapi...

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Autores principales:	V. V. Neroev, N. V. Balatskaya, A. Yu. Novikova, M. V. Ryabina, P. A. Ilyukhin
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2020
Materias:	retinal capillary hemangioma vhl gene von hippel-lindau syndrome pathogenesis cytokines Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/f123eff217614662834c83b721d64ed4
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spelling	oai:doaj.org-article:f123eff217614662834c83b721d64ed42021-11-18T08:03:50ZProven and less studied hematopoietic and vasoactive growth factors in retinal capillary hemangioma1563-06252313-741X10.15789/1563-0625-PAL-2002https://doaj.org/article/f123eff217614662834c83b721d64ed42020-12-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/2002https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XPathogenesis of retinal capillary hemangioma has not been sufficiently studied at the present time. Therefore, the study of cytokine levels in biological fluids seems to be very relevant in order to increase knowledge about the mechanisms of the disease development and searching for targeted therapies. The content of hematopoietic and vasoactive growth factors in blood serum, lacrimal fluid, and vitreous body was studied in patients with retinal capillary hemangioma. A total of 26 patients with retinal angiomatosis were examined. The samples of blood serum (n = 23) and lacrimal fluid (n = 10) from practically healthy people aged 22 to 46 (27.4±1.4 years) were used as a control. To perform comparative assessment of cytokine concentrations in the vitreous body of patients with retinal capillary hemangioma, were used samples of the vitreous body from 6 patients (average age 33±4.7 years; from 21 to 49 years) with rhegmatogenous retinal detachment. To measure the cytokine concentrations, we applied multiplex analysis technique using the xMAP platform with LuminexxPONENT 3.1 program and ProcartaPlex sets (eBioscience, Austria). A detailed characteristic of vasoactive factors in capillary retinal hemangioma was obtained as a result of this work. Some disorders in chemokine regulation were identified. There was a significant increase in serum concentrations of three vasoactive factors, i.e., PDGF-BB, HGF, and PIGF-1, with a decrease in chemokines (MCP-1, MIP-1α, and MIP-1β). The frequencies of PIGF-1 and MIP-1α detection also significantly differed from the control group. SCF was significantly more often determined in patients with retinal angiomatosis only at the systemic level. Correlations between PDGF-BB and PIGF-1, as well as PIGF-1 and MIP-1β were shown. A significant increase in VEGF-A, HGF, VEGF-D, as well as MCP-1 concentrations was shown in the lacrimal fluid. The inversion of PDGF-BB concentrations in serum and lacrimal fluid was noted. Analysis of intraocular cytokine levels revealed a significant increase in VEGF-A and HGF concentrations, with marked decrease in MIP-1α and MIP-1β. PDGF-BB in 100% of cases was determined only in vitreous body of patients with retinal angiomatosis. With respect to the revealed characteristic shifts of HGF/SF intraocular production in retinal capillary hemangioma, it seems relevant to search ways for its inhibition, thus providing potential basis for a new therapeutic strategy in treatment of retinal angiomatosis.V. V. NeroevN. V. BalatskayaA. Yu. NovikovaM. V. RyabinaP. A. IlyukhinSPb RAACIarticleretinal capillary hemangiomavhl genevon hippel-lindau syndromepathogenesiscytokinesImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 22, Iss 5, Pp 943-956 (2020)
institution	DOAJ
collection	DOAJ
language	RU
topic	retinal capillary hemangioma vhl gene von hippel-lindau syndrome pathogenesis cytokines Immunologic diseases. Allergy RC581-607
spellingShingle	retinal capillary hemangioma vhl gene von hippel-lindau syndrome pathogenesis cytokines Immunologic diseases. Allergy RC581-607 V. V. Neroev N. V. Balatskaya A. Yu. Novikova M. V. Ryabina P. A. Ilyukhin Proven and less studied hematopoietic and vasoactive growth factors in retinal capillary hemangioma
description	Pathogenesis of retinal capillary hemangioma has not been sufficiently studied at the present time. Therefore, the study of cytokine levels in biological fluids seems to be very relevant in order to increase knowledge about the mechanisms of the disease development and searching for targeted therapies. The content of hematopoietic and vasoactive growth factors in blood serum, lacrimal fluid, and vitreous body was studied in patients with retinal capillary hemangioma. A total of 26 patients with retinal angiomatosis were examined. The samples of blood serum (n = 23) and lacrimal fluid (n = 10) from practically healthy people aged 22 to 46 (27.4±1.4 years) were used as a control. To perform comparative assessment of cytokine concentrations in the vitreous body of patients with retinal capillary hemangioma, were used samples of the vitreous body from 6 patients (average age 33±4.7 years; from 21 to 49 years) with rhegmatogenous retinal detachment. To measure the cytokine concentrations, we applied multiplex analysis technique using the xMAP platform with LuminexxPONENT 3.1 program and ProcartaPlex sets (eBioscience, Austria). A detailed characteristic of vasoactive factors in capillary retinal hemangioma was obtained as a result of this work. Some disorders in chemokine regulation were identified. There was a significant increase in serum concentrations of three vasoactive factors, i.e., PDGF-BB, HGF, and PIGF-1, with a decrease in chemokines (MCP-1, MIP-1α, and MIP-1β). The frequencies of PIGF-1 and MIP-1α detection also significantly differed from the control group. SCF was significantly more often determined in patients with retinal angiomatosis only at the systemic level. Correlations between PDGF-BB and PIGF-1, as well as PIGF-1 and MIP-1β were shown. A significant increase in VEGF-A, HGF, VEGF-D, as well as MCP-1 concentrations was shown in the lacrimal fluid. The inversion of PDGF-BB concentrations in serum and lacrimal fluid was noted. Analysis of intraocular cytokine levels revealed a significant increase in VEGF-A and HGF concentrations, with marked decrease in MIP-1α and MIP-1β. PDGF-BB in 100% of cases was determined only in vitreous body of patients with retinal angiomatosis. With respect to the revealed characteristic shifts of HGF/SF intraocular production in retinal capillary hemangioma, it seems relevant to search ways for its inhibition, thus providing potential basis for a new therapeutic strategy in treatment of retinal angiomatosis.
format	article
author	V. V. Neroev N. V. Balatskaya A. Yu. Novikova M. V. Ryabina P. A. Ilyukhin
author_facet	V. V. Neroev N. V. Balatskaya A. Yu. Novikova M. V. Ryabina P. A. Ilyukhin
author_sort	V. V. Neroev
title	Proven and less studied hematopoietic and vasoactive growth factors in retinal capillary hemangioma
title_short	Proven and less studied hematopoietic and vasoactive growth factors in retinal capillary hemangioma
title_full	Proven and less studied hematopoietic and vasoactive growth factors in retinal capillary hemangioma
title_fullStr	Proven and less studied hematopoietic and vasoactive growth factors in retinal capillary hemangioma
title_full_unstemmed	Proven and less studied hematopoietic and vasoactive growth factors in retinal capillary hemangioma
title_sort	proven and less studied hematopoietic and vasoactive growth factors in retinal capillary hemangioma
publisher	SPb RAACI
publishDate	2020
url	https://doaj.org/article/f123eff217614662834c83b721d64ed4
work_keys_str_mv	AT vvneroev provenandlessstudiedhematopoieticandvasoactivegrowthfactorsinretinalcapillaryhemangioma AT nvbalatskaya provenandlessstudiedhematopoieticandvasoactivegrowthfactorsinretinalcapillaryhemangioma AT ayunovikova provenandlessstudiedhematopoieticandvasoactivegrowthfactorsinretinalcapillaryhemangioma AT mvryabina provenandlessstudiedhematopoieticandvasoactivegrowthfactorsinretinalcapillaryhemangioma AT pailyukhin provenandlessstudiedhematopoieticandvasoactivegrowthfactorsinretinalcapillaryhemangioma
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Proven and less studied hematopoietic and vasoactive growth factors in retinal capillary hemangioma

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