Membrane lipidome reorganization correlates with the fate of neuroblastoma cells supplemented with fatty acids.

Palmitic acid is known to be apoptotic for nervous cells but no data are available on membrane lipidome transformations occurring during its supplementation, although membrane lipids are clearly involved in the apoptotic signaling cascade. NB100 neuroblastoma cells were supplemented with palmitic ac...

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Autores principales:	Andrea Bolognesi, Alexandros Chatgilialoglu, Letizia Polito, Carla Ferreri
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f123f81df71d4c9eb92bb0cec442a1d6
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spelling	oai:doaj.org-article:f123f81df71d4c9eb92bb0cec442a1d62021-11-18T07:58:35ZMembrane lipidome reorganization correlates with the fate of neuroblastoma cells supplemented with fatty acids.1932-620310.1371/journal.pone.0055537https://doaj.org/article/f123f81df71d4c9eb92bb0cec442a1d62013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23405167/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Palmitic acid is known to be apoptotic for nervous cells but no data are available on membrane lipidome transformations occurring during its supplementation, although membrane lipids are clearly involved in the apoptotic signaling cascade. NB100 neuroblastoma cells were supplemented with palmitic acid and membrane fatty acids were isolated, derivatized and analysed by gas chromatography at defined time intervals. Parallely, cell viability, morphology, apoptosis, cPLA(2) and caspase activations were checked. Interestingly, under 150 µM supplementation the incorporation of palmitic acid was accompanied by the specific release of arachidonic acid. This event was timely correlated with cPLA(2) and caspases activations, and the time window of 60 minutes was envisaged for crucial membrane lipidome changes. The simultaneous addition of 50 µM oleic, 50 µM arachidonic and 150 µM palmitic acids to the cell cultures influenced membrane changes with suppression of caspase activation and maintenance of cell viability. These results highlight the role of the membrane asset with fatty acid remodeling and suggest the potential of lipid-based strategies for influencing cell response and fate in human diseases, such as neurodegenerative disorders or tumours.Andrea BolognesiAlexandros ChatgilialogluLetizia PolitoCarla FerreriPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 2, p e55537 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Andrea Bolognesi Alexandros Chatgilialoglu Letizia Polito Carla Ferreri Membrane lipidome reorganization correlates with the fate of neuroblastoma cells supplemented with fatty acids.
description	Palmitic acid is known to be apoptotic for nervous cells but no data are available on membrane lipidome transformations occurring during its supplementation, although membrane lipids are clearly involved in the apoptotic signaling cascade. NB100 neuroblastoma cells were supplemented with palmitic acid and membrane fatty acids were isolated, derivatized and analysed by gas chromatography at defined time intervals. Parallely, cell viability, morphology, apoptosis, cPLA(2) and caspase activations were checked. Interestingly, under 150 µM supplementation the incorporation of palmitic acid was accompanied by the specific release of arachidonic acid. This event was timely correlated with cPLA(2) and caspases activations, and the time window of 60 minutes was envisaged for crucial membrane lipidome changes. The simultaneous addition of 50 µM oleic, 50 µM arachidonic and 150 µM palmitic acids to the cell cultures influenced membrane changes with suppression of caspase activation and maintenance of cell viability. These results highlight the role of the membrane asset with fatty acid remodeling and suggest the potential of lipid-based strategies for influencing cell response and fate in human diseases, such as neurodegenerative disorders or tumours.
format	article
author	Andrea Bolognesi Alexandros Chatgilialoglu Letizia Polito Carla Ferreri
author_facet	Andrea Bolognesi Alexandros Chatgilialoglu Letizia Polito Carla Ferreri
author_sort	Andrea Bolognesi
title	Membrane lipidome reorganization correlates with the fate of neuroblastoma cells supplemented with fatty acids.
title_short	Membrane lipidome reorganization correlates with the fate of neuroblastoma cells supplemented with fatty acids.
title_full	Membrane lipidome reorganization correlates with the fate of neuroblastoma cells supplemented with fatty acids.
title_fullStr	Membrane lipidome reorganization correlates with the fate of neuroblastoma cells supplemented with fatty acids.
title_full_unstemmed	Membrane lipidome reorganization correlates with the fate of neuroblastoma cells supplemented with fatty acids.
title_sort	membrane lipidome reorganization correlates with the fate of neuroblastoma cells supplemented with fatty acids.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/f123f81df71d4c9eb92bb0cec442a1d6
work_keys_str_mv	AT andreabolognesi membranelipidomereorganizationcorrelateswiththefateofneuroblastomacellssupplementedwithfattyacids AT alexandroschatgilialoglu membranelipidomereorganizationcorrelateswiththefateofneuroblastomacellssupplementedwithfattyacids AT letiziapolito membranelipidomereorganizationcorrelateswiththefateofneuroblastomacellssupplementedwithfattyacids AT carlaferreri membranelipidomereorganizationcorrelateswiththefateofneuroblastomacellssupplementedwithfattyacids
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Membrane lipidome reorganization correlates with the fate of neuroblastoma cells supplemented with fatty acids.

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