Synergistic and additive effects of epigallocatechin gallate and digitonin on Plasmodium sporozoite survival and motility.

<h4>Background</h4>Most medicinal plants contain a mixture of bioactive compounds, including chemicals that interact with intracellular targets and others that can act as adjuvants to facilitate absorption of polar agents across cellular membranes. However, little is known about synergis...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Janina K Hellmann, Sylvia Münter, Michael Wink, Friedrich Frischknecht
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f129cbd08d4b4d59a51d7463f29d46ee
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f129cbd08d4b4d59a51d7463f29d46ee
record_format	dspace
spelling	oai:doaj.org-article:f129cbd08d4b4d59a51d7463f29d46ee2021-11-25T06:26:48ZSynergistic and additive effects of epigallocatechin gallate and digitonin on Plasmodium sporozoite survival and motility.1932-620310.1371/journal.pone.0008682https://doaj.org/article/f129cbd08d4b4d59a51d7463f29d46ee2010-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20072627/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Most medicinal plants contain a mixture of bioactive compounds, including chemicals that interact with intracellular targets and others that can act as adjuvants to facilitate absorption of polar agents across cellular membranes. However, little is known about synergistic effects between such potential drug candidates and adjuvants. To probe for such effects, we tested the green tea compound epigallocatechin gallate (EGCG) and the membrane permeabilising digitonin on Plasmodium sporozoite motility and viability.<h4>Methodology/principal findings</h4>Green fluorescent P. berghei sporozoites were imaged using a recently developed visual screening methodology. Motility and viability parameters were automatically analyzed and IC50 values were calculated, and the synergism of drug and adjuvant was assessed by the fractional inhibitory concentration index. Validating our visual screening procedure, we showed that sporozoite motility and liver cell infection is inhibited by EGCG at nontoxic concentrations. Digitonin synergistically increases the cytotoxicity of EGCG on sporozoite survival, but shows an additive effect on sporozoite motility.<h4>Conclusions/significance</h4>We proved the feasibility of performing highly reliable visual screens for compounds against Plasmodium sporozoites. We thereby could show an advantage of administering mixtures of plant metabolites on inhibition of cell motility and survival. Although the effective concentration of both drugs is too high for use in malaria prophylaxis, the demonstration of a synergistic effect between two plant compounds could lead to new avenues in drug discovery.Janina K HellmannSylvia MünterMichael WinkFriedrich FrischknechtPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 1, p e8682 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Janina K Hellmann Sylvia Münter Michael Wink Friedrich Frischknecht Synergistic and additive effects of epigallocatechin gallate and digitonin on Plasmodium sporozoite survival and motility.
description	<h4>Background</h4>Most medicinal plants contain a mixture of bioactive compounds, including chemicals that interact with intracellular targets and others that can act as adjuvants to facilitate absorption of polar agents across cellular membranes. However, little is known about synergistic effects between such potential drug candidates and adjuvants. To probe for such effects, we tested the green tea compound epigallocatechin gallate (EGCG) and the membrane permeabilising digitonin on Plasmodium sporozoite motility and viability.<h4>Methodology/principal findings</h4>Green fluorescent P. berghei sporozoites were imaged using a recently developed visual screening methodology. Motility and viability parameters were automatically analyzed and IC50 values were calculated, and the synergism of drug and adjuvant was assessed by the fractional inhibitory concentration index. Validating our visual screening procedure, we showed that sporozoite motility and liver cell infection is inhibited by EGCG at nontoxic concentrations. Digitonin synergistically increases the cytotoxicity of EGCG on sporozoite survival, but shows an additive effect on sporozoite motility.<h4>Conclusions/significance</h4>We proved the feasibility of performing highly reliable visual screens for compounds against Plasmodium sporozoites. We thereby could show an advantage of administering mixtures of plant metabolites on inhibition of cell motility and survival. Although the effective concentration of both drugs is too high for use in malaria prophylaxis, the demonstration of a synergistic effect between two plant compounds could lead to new avenues in drug discovery.
format	article
author	Janina K Hellmann Sylvia Münter Michael Wink Friedrich Frischknecht
author_facet	Janina K Hellmann Sylvia Münter Michael Wink Friedrich Frischknecht
author_sort	Janina K Hellmann
title	Synergistic and additive effects of epigallocatechin gallate and digitonin on Plasmodium sporozoite survival and motility.
title_short	Synergistic and additive effects of epigallocatechin gallate and digitonin on Plasmodium sporozoite survival and motility.
title_full	Synergistic and additive effects of epigallocatechin gallate and digitonin on Plasmodium sporozoite survival and motility.
title_fullStr	Synergistic and additive effects of epigallocatechin gallate and digitonin on Plasmodium sporozoite survival and motility.
title_full_unstemmed	Synergistic and additive effects of epigallocatechin gallate and digitonin on Plasmodium sporozoite survival and motility.
title_sort	synergistic and additive effects of epigallocatechin gallate and digitonin on plasmodium sporozoite survival and motility.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/f129cbd08d4b4d59a51d7463f29d46ee
work_keys_str_mv	AT janinakhellmann synergisticandadditiveeffectsofepigallocatechingallateanddigitoninonplasmodiumsporozoitesurvivalandmotility AT sylviamunter synergisticandadditiveeffectsofepigallocatechingallateanddigitoninonplasmodiumsporozoitesurvivalandmotility AT michaelwink synergisticandadditiveeffectsofepigallocatechingallateanddigitoninonplasmodiumsporozoitesurvivalandmotility AT friedrichfrischknecht synergisticandadditiveeffectsofepigallocatechingallateanddigitoninonplasmodiumsporozoitesurvivalandmotility
_version_	1718413716514406400

Synergistic and additive effects of epigallocatechin gallate and digitonin on Plasmodium sporozoite survival and motility.

Ejemplares similares