Precise stacking of decellularized extracellular matrix based 3D cell-laden constructs by a 3D cell printing system equipped with heating modules

Abstract Three-dimensional (3D) cell printing systems allow the controlled and precise deposition of multiple cells in 3D constructs. Hydrogel materials have been used extensively as printable bioinks owing to their ability to safely encapsulate living cells. However, hydrogel-based bioinks have dra...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Geunseon Ahn, Kyung-Hyun Min, Changhwan Kim, Jeong-Seok Lee, Donggu Kang, Joo-Yun Won, Dong-Woo Cho, Jun-Young Kim, Songwan Jin, Won-Soo Yun, Jin-Hyung Shim
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f12c1e0bf86e42eda5728db988bb7169
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f12c1e0bf86e42eda5728db988bb7169
record_format	dspace
spelling	oai:doaj.org-article:f12c1e0bf86e42eda5728db988bb71692021-12-02T11:40:41ZPrecise stacking of decellularized extracellular matrix based 3D cell-laden constructs by a 3D cell printing system equipped with heating modules10.1038/s41598-017-09201-52045-2322https://doaj.org/article/f12c1e0bf86e42eda5728db988bb71692017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-09201-5https://doaj.org/toc/2045-2322Abstract Three-dimensional (3D) cell printing systems allow the controlled and precise deposition of multiple cells in 3D constructs. Hydrogel materials have been used extensively as printable bioinks owing to their ability to safely encapsulate living cells. However, hydrogel-based bioinks have drawbacks for cell printing, e.g. inappropriate crosslinking and liquid-like rheological properties, which hinder precise 3D shaping. Therefore, in this study, we investigated the influence of various factors (e.g. bioink concentration, viscosity, and extent of crosslinking) on cell printing and established a new 3D cell printing system equipped with heating modules for the precise stacking of decellularized extracellular matrix (dECM)-based 3D cell-laden constructs. Because the pH-adjusted bioink isolated from native tissue is safely gelled at 37 °C, our heating system facilitated the precise stacking of dECM bioinks by enabling simultaneous gelation during printing. We observed greater printability compared with that of a non-heating system. These results were confirmed by mechanical testing and 3D construct stacking analyses. We also confirmed that our heating system did not elicit negative effects, such as cell death, in the printed cells. Conclusively, these results hold promise for the application of 3D bioprinting to tissue engineering and drug development.Geunseon AhnKyung-Hyun MinChanghwan KimJeong-Seok LeeDonggu KangJoo-Yun WonDong-Woo ChoJun-Young KimSongwan JinWon-Soo YunJin-Hyung ShimNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Geunseon Ahn Kyung-Hyun Min Changhwan Kim Jeong-Seok Lee Donggu Kang Joo-Yun Won Dong-Woo Cho Jun-Young Kim Songwan Jin Won-Soo Yun Jin-Hyung Shim Precise stacking of decellularized extracellular matrix based 3D cell-laden constructs by a 3D cell printing system equipped with heating modules
description	Abstract Three-dimensional (3D) cell printing systems allow the controlled and precise deposition of multiple cells in 3D constructs. Hydrogel materials have been used extensively as printable bioinks owing to their ability to safely encapsulate living cells. However, hydrogel-based bioinks have drawbacks for cell printing, e.g. inappropriate crosslinking and liquid-like rheological properties, which hinder precise 3D shaping. Therefore, in this study, we investigated the influence of various factors (e.g. bioink concentration, viscosity, and extent of crosslinking) on cell printing and established a new 3D cell printing system equipped with heating modules for the precise stacking of decellularized extracellular matrix (dECM)-based 3D cell-laden constructs. Because the pH-adjusted bioink isolated from native tissue is safely gelled at 37 °C, our heating system facilitated the precise stacking of dECM bioinks by enabling simultaneous gelation during printing. We observed greater printability compared with that of a non-heating system. These results were confirmed by mechanical testing and 3D construct stacking analyses. We also confirmed that our heating system did not elicit negative effects, such as cell death, in the printed cells. Conclusively, these results hold promise for the application of 3D bioprinting to tissue engineering and drug development.
format	article
author	Geunseon Ahn Kyung-Hyun Min Changhwan Kim Jeong-Seok Lee Donggu Kang Joo-Yun Won Dong-Woo Cho Jun-Young Kim Songwan Jin Won-Soo Yun Jin-Hyung Shim
author_facet	Geunseon Ahn Kyung-Hyun Min Changhwan Kim Jeong-Seok Lee Donggu Kang Joo-Yun Won Dong-Woo Cho Jun-Young Kim Songwan Jin Won-Soo Yun Jin-Hyung Shim
author_sort	Geunseon Ahn
title	Precise stacking of decellularized extracellular matrix based 3D cell-laden constructs by a 3D cell printing system equipped with heating modules
title_short	Precise stacking of decellularized extracellular matrix based 3D cell-laden constructs by a 3D cell printing system equipped with heating modules
title_full	Precise stacking of decellularized extracellular matrix based 3D cell-laden constructs by a 3D cell printing system equipped with heating modules
title_fullStr	Precise stacking of decellularized extracellular matrix based 3D cell-laden constructs by a 3D cell printing system equipped with heating modules
title_full_unstemmed	Precise stacking of decellularized extracellular matrix based 3D cell-laden constructs by a 3D cell printing system equipped with heating modules
title_sort	precise stacking of decellularized extracellular matrix based 3d cell-laden constructs by a 3d cell printing system equipped with heating modules
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/f12c1e0bf86e42eda5728db988bb7169
work_keys_str_mv	AT geunseonahn precisestackingofdecellularizedextracellularmatrixbased3dcellladenconstructsbya3dcellprintingsystemequippedwithheatingmodules AT kyunghyunmin precisestackingofdecellularizedextracellularmatrixbased3dcellladenconstructsbya3dcellprintingsystemequippedwithheatingmodules AT changhwankim precisestackingofdecellularizedextracellularmatrixbased3dcellladenconstructsbya3dcellprintingsystemequippedwithheatingmodules AT jeongseoklee precisestackingofdecellularizedextracellularmatrixbased3dcellladenconstructsbya3dcellprintingsystemequippedwithheatingmodules AT donggukang precisestackingofdecellularizedextracellularmatrixbased3dcellladenconstructsbya3dcellprintingsystemequippedwithheatingmodules AT jooyunwon precisestackingofdecellularizedextracellularmatrixbased3dcellladenconstructsbya3dcellprintingsystemequippedwithheatingmodules AT dongwoocho precisestackingofdecellularizedextracellularmatrixbased3dcellladenconstructsbya3dcellprintingsystemequippedwithheatingmodules AT junyoungkim precisestackingofdecellularizedextracellularmatrixbased3dcellladenconstructsbya3dcellprintingsystemequippedwithheatingmodules AT songwanjin precisestackingofdecellularizedextracellularmatrixbased3dcellladenconstructsbya3dcellprintingsystemequippedwithheatingmodules AT wonsooyun precisestackingofdecellularizedextracellularmatrixbased3dcellladenconstructsbya3dcellprintingsystemequippedwithheatingmodules AT jinhyungshim precisestackingofdecellularizedextracellularmatrixbased3dcellladenconstructsbya3dcellprintingsystemequippedwithheatingmodules
_version_	1718395617005273088

Precise stacking of decellularized extracellular matrix based 3D cell-laden constructs by a 3D cell printing system equipped with heating modules

Ejemplares similares