Detection of Borrelia burgdorferi antigens in tissues and plasma during early infection in a mouse model

Abstract Borrelia burgdorferi is the causative agent of Lyme borreliosis, which is the most common tick-borne human disease in Europe and North America. Currently, the diagnosis of Lyme borreliosis is based on serological tests allowing indirect detection of anti-Borrelia antibodies produced by pati...

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Autores principales:	Victoria Dolange, Stéphanie Simon, Nathalie Morel
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f131a7619ebf4bcd9e7e10f90a096bfe
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spelling	oai:doaj.org-article:f131a7619ebf4bcd9e7e10f90a096bfe2021-12-02T17:51:16ZDetection of Borrelia burgdorferi antigens in tissues and plasma during early infection in a mouse model10.1038/s41598-021-96861-z2045-2322https://doaj.org/article/f131a7619ebf4bcd9e7e10f90a096bfe2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96861-zhttps://doaj.org/toc/2045-2322Abstract Borrelia burgdorferi is the causative agent of Lyme borreliosis, which is the most common tick-borne human disease in Europe and North America. Currently, the diagnosis of Lyme borreliosis is based on serological tests allowing indirect detection of anti-Borrelia antibodies produced by patients. Their main drawback is a lack of sensitivity in the early phase of disease and an incapacity to prove an active infection. Direct diagnostic tests are clearly needed. The objectives of this study were to produce tools allowing sensitive detection of potential circulating Borrelia antigens and to evaluate them in a mouse model. We focused on two potential early bacterial makers, the highly variable OspC protein and the conserved protein FlaB. High-affinity monoclonal antibodies were produced and used to establish various immunoassays and western blot detection. A very good limit of detection for OspC as low as 17 pg/mL of sample was achieved with SPIE-IA. In infected mice, we were able to measure OspC in plasma with a mean value of 10 ng/mL at 7 days post-inoculation. This result suggests that OspC could be a good blood marker for diagnosis of Lyme borreliosis and that the tools developed during this study could be very useful.Victoria DolangeStéphanie SimonNathalie MorelNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Victoria Dolange Stéphanie Simon Nathalie Morel Detection of Borrelia burgdorferi antigens in tissues and plasma during early infection in a mouse model
description	Abstract Borrelia burgdorferi is the causative agent of Lyme borreliosis, which is the most common tick-borne human disease in Europe and North America. Currently, the diagnosis of Lyme borreliosis is based on serological tests allowing indirect detection of anti-Borrelia antibodies produced by patients. Their main drawback is a lack of sensitivity in the early phase of disease and an incapacity to prove an active infection. Direct diagnostic tests are clearly needed. The objectives of this study were to produce tools allowing sensitive detection of potential circulating Borrelia antigens and to evaluate them in a mouse model. We focused on two potential early bacterial makers, the highly variable OspC protein and the conserved protein FlaB. High-affinity monoclonal antibodies were produced and used to establish various immunoassays and western blot detection. A very good limit of detection for OspC as low as 17 pg/mL of sample was achieved with SPIE-IA. In infected mice, we were able to measure OspC in plasma with a mean value of 10 ng/mL at 7 days post-inoculation. This result suggests that OspC could be a good blood marker for diagnosis of Lyme borreliosis and that the tools developed during this study could be very useful.
format	article
author	Victoria Dolange Stéphanie Simon Nathalie Morel
author_facet	Victoria Dolange Stéphanie Simon Nathalie Morel
author_sort	Victoria Dolange
title	Detection of Borrelia burgdorferi antigens in tissues and plasma during early infection in a mouse model
title_short	Detection of Borrelia burgdorferi antigens in tissues and plasma during early infection in a mouse model
title_full	Detection of Borrelia burgdorferi antigens in tissues and plasma during early infection in a mouse model
title_fullStr	Detection of Borrelia burgdorferi antigens in tissues and plasma during early infection in a mouse model
title_full_unstemmed	Detection of Borrelia burgdorferi antigens in tissues and plasma during early infection in a mouse model
title_sort	detection of borrelia burgdorferi antigens in tissues and plasma during early infection in a mouse model
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f131a7619ebf4bcd9e7e10f90a096bfe
work_keys_str_mv	AT victoriadolange detectionofborreliaburgdorferiantigensintissuesandplasmaduringearlyinfectioninamousemodel AT stephaniesimon detectionofborreliaburgdorferiantigensintissuesandplasmaduringearlyinfectioninamousemodel AT nathaliemorel detectionofborreliaburgdorferiantigensintissuesandplasmaduringearlyinfectioninamousemodel
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Detection of Borrelia burgdorferi antigens in tissues and plasma during early infection in a mouse model

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