Temporal expression study of miRNAs in the crown tissues of winter wheat grown under natural growth conditions
Abstract Background Winter wheat requires prolonged exposure to low temperature to initiate flowering (vernalization). Shoot apical meristem of the crown is the site of cold perception, which produces leaf primordia during vegetative growth before developing into floral primordia at the initiation o...
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oai:doaj.org-article:f13ca2cb19484e9890c37ed0f39702002021-11-08T10:57:19ZTemporal expression study of miRNAs in the crown tissues of winter wheat grown under natural growth conditions10.1186/s12864-021-08048-51471-2164https://doaj.org/article/f13ca2cb19484e9890c37ed0f39702002021-11-01T00:00:00Zhttps://doi.org/10.1186/s12864-021-08048-5https://doaj.org/toc/1471-2164Abstract Background Winter wheat requires prolonged exposure to low temperature to initiate flowering (vernalization). Shoot apical meristem of the crown is the site of cold perception, which produces leaf primordia during vegetative growth before developing into floral primordia at the initiation of the reproductive phase. Although many essential genes for winter wheat cold acclimation and floral initiation have been revealed, the importance of microRNA (miRNA) meditated post-transcriptional regulation in crowns is not well understood. To understand the potential roles of miRNAs in crown tissues, we performed a temporal expression study of miRNAs in crown tissues at the three-leaf stage, winter dormancy stage, spring green-up stage, and jointing stage of winter wheat grown under natural growth conditions. Results In total, 348 miRNAs belonging to 298 miRNA families, were identified in wheat crown tissues. Among them, 92 differentially expressed miRNAs (DEMs) were found to be significantly regulated from the three-leaf stage to the jointing stage. Most of these DEMs were highly expressed at the three-leaf stage and winter dormancy stage, and then declined in later stages. Six DEMs, including miR156a-5p were markedly induced during the winter dormancy stage. Eleven DEMs, including miR159a.1, miR390a-5p, miR393-5p, miR160a-5p, and miR1436, were highly expressed at the green-up stage. Twelve DEMs, such as miR172a-5p, miR394a, miR319b-3p, and miR9676-5p were highly induced at the jointing stage. Moreover, 14 novel target genes of nine wheat or Pooideae-specific miRNAs were verified using RLM-5′ RACE assay. Notably, six mTERFs and two Rf1 genes, which are associated with mitochondrial gene expression, were confirmed as targets of three wheat-specific miRNAs. Conclusions The present study not only confirmed the known miRNAs associated with phase transition and floral development, but also identified a number of wheat or Pooideae-specific miRNAs critical for winter wheat cold acclimation and floral development. Most importantly, this study provided experimental evidence that miRNA could regulate mitochondrial gene expression by targeting mTERF and Rf1 genes. Our study provides valuable information for further exploration of the mechanism of miRNA mediated post-transcriptional regulation during winter wheat vernalization and inflorescent initiation.Menglei WangChenhui YangKangning WeiMiao ZhaoLiqiang ShenJie JiLi WangDaijing ZhangJunqiang GuoYun ZhengJuanjuan YuMo ZhuHaiying LiuYong-Fang LiBMCarticleWinter wheatmiRNACrownTarget geneCold acclimationPost-transcriptional regulationBiotechnologyTP248.13-248.65GeneticsQH426-470ENBMC Genomics, Vol 22, Iss S3, Pp 1-15 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Winter wheat miRNA Crown Target gene Cold acclimation Post-transcriptional regulation Biotechnology TP248.13-248.65 Genetics QH426-470 |
| spellingShingle |
Winter wheat miRNA Crown Target gene Cold acclimation Post-transcriptional regulation Biotechnology TP248.13-248.65 Genetics QH426-470 Menglei Wang Chenhui Yang Kangning Wei Miao Zhao Liqiang Shen Jie Ji Li Wang Daijing Zhang Junqiang Guo Yun Zheng Juanjuan Yu Mo Zhu Haiying Liu Yong-Fang Li Temporal expression study of miRNAs in the crown tissues of winter wheat grown under natural growth conditions |
| description |
Abstract Background Winter wheat requires prolonged exposure to low temperature to initiate flowering (vernalization). Shoot apical meristem of the crown is the site of cold perception, which produces leaf primordia during vegetative growth before developing into floral primordia at the initiation of the reproductive phase. Although many essential genes for winter wheat cold acclimation and floral initiation have been revealed, the importance of microRNA (miRNA) meditated post-transcriptional regulation in crowns is not well understood. To understand the potential roles of miRNAs in crown tissues, we performed a temporal expression study of miRNAs in crown tissues at the three-leaf stage, winter dormancy stage, spring green-up stage, and jointing stage of winter wheat grown under natural growth conditions. Results In total, 348 miRNAs belonging to 298 miRNA families, were identified in wheat crown tissues. Among them, 92 differentially expressed miRNAs (DEMs) were found to be significantly regulated from the three-leaf stage to the jointing stage. Most of these DEMs were highly expressed at the three-leaf stage and winter dormancy stage, and then declined in later stages. Six DEMs, including miR156a-5p were markedly induced during the winter dormancy stage. Eleven DEMs, including miR159a.1, miR390a-5p, miR393-5p, miR160a-5p, and miR1436, were highly expressed at the green-up stage. Twelve DEMs, such as miR172a-5p, miR394a, miR319b-3p, and miR9676-5p were highly induced at the jointing stage. Moreover, 14 novel target genes of nine wheat or Pooideae-specific miRNAs were verified using RLM-5′ RACE assay. Notably, six mTERFs and two Rf1 genes, which are associated with mitochondrial gene expression, were confirmed as targets of three wheat-specific miRNAs. Conclusions The present study not only confirmed the known miRNAs associated with phase transition and floral development, but also identified a number of wheat or Pooideae-specific miRNAs critical for winter wheat cold acclimation and floral development. Most importantly, this study provided experimental evidence that miRNA could regulate mitochondrial gene expression by targeting mTERF and Rf1 genes. Our study provides valuable information for further exploration of the mechanism of miRNA mediated post-transcriptional regulation during winter wheat vernalization and inflorescent initiation. |
| format |
article |
| author |
Menglei Wang Chenhui Yang Kangning Wei Miao Zhao Liqiang Shen Jie Ji Li Wang Daijing Zhang Junqiang Guo Yun Zheng Juanjuan Yu Mo Zhu Haiying Liu Yong-Fang Li |
| author_facet |
Menglei Wang Chenhui Yang Kangning Wei Miao Zhao Liqiang Shen Jie Ji Li Wang Daijing Zhang Junqiang Guo Yun Zheng Juanjuan Yu Mo Zhu Haiying Liu Yong-Fang Li |
| author_sort |
Menglei Wang |
| title |
Temporal expression study of miRNAs in the crown tissues of winter wheat grown under natural growth conditions |
| title_short |
Temporal expression study of miRNAs in the crown tissues of winter wheat grown under natural growth conditions |
| title_full |
Temporal expression study of miRNAs in the crown tissues of winter wheat grown under natural growth conditions |
| title_fullStr |
Temporal expression study of miRNAs in the crown tissues of winter wheat grown under natural growth conditions |
| title_full_unstemmed |
Temporal expression study of miRNAs in the crown tissues of winter wheat grown under natural growth conditions |
| title_sort |
temporal expression study of mirnas in the crown tissues of winter wheat grown under natural growth conditions |
| publisher |
BMC |
| publishDate |
2021 |
| url |
https://doaj.org/article/f13ca2cb19484e9890c37ed0f3970200 |
| work_keys_str_mv |
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| _version_ |
1718442483174604800 |