Centrifugally automated Solid-Phase Extraction of DNA by immiscible liquid valving and chemically powered centripetal pumping of peripherally stored reagents

This paper presents two flow-control technologies for use on centrifugal Lab-on-a-Disc systems. The first, immiscible liquid valving, selectively blocks microfluidic channels using a high-density liquid fluorocarbon (FC-40). Used with a specific channel geometry, the FC-40 can permit liquid to enter...

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Autores principales:	David J. Kinahan, Robert Burger, Daryl Lawlor, Philip L. Early, Abhishek Vembadi, Niamh A. McArdle, Niamh A. Kilcawley, Macdara T. Glynn, Jens Ducrée
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Centrifugal microfluidics Lab-on-a-disc Centripetal pumping Check valves Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/f13e5b8f99e149edad5df481a2a6e9b4
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spelling	oai:doaj.org-article:f13e5b8f99e149edad5df481a2a6e9b42021-11-06T04:35:50ZCentrifugally automated Solid-Phase Extraction of DNA by immiscible liquid valving and chemically powered centripetal pumping of peripherally stored reagents2590-137010.1016/j.biosx.2021.100085https://doaj.org/article/f13e5b8f99e149edad5df481a2a6e9b42021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2590137021000212https://doaj.org/toc/2590-1370This paper presents two flow-control technologies for use on centrifugal Lab-on-a-Disc systems. The first, immiscible liquid valving, selectively blocks microfluidic channels using a high-density liquid fluorocarbon (FC-40). Used with a specific channel geometry, the FC-40 can permit liquid to enter a chamber but prevents it flowing back along the same path and so acts as “liquid” check-valve. The same liquid can be combined with a water-dissolvable film to provide an extremely robust liquid routing structure. The second technology uses CO2 gas, created by wetting of commodity baking powder by water, to centripetally pump liquid from the periphery of the disc to the centre of the disc. The technologies are combined with valving schemes based on strategically placed, solvent-selective dissolvable films (DFs) to demonstrate repeated pumping of a liquid sample from the edge of the disc to the centre of the disc. The flow-control technologies are then combined to demonstrate fully automated Solid-Phase Extraction (SPE) of DNA with reagent storage on the periphery of the disc. We report an extraction efficiency of 47% measured relative to commercial spin-columns.David J. KinahanRobert BurgerDaryl LawlorPhilip L. EarlyAbhishek VembadiNiamh A. McArdleNiamh A. KilcawleyMacdara T. GlynnJens DucréeElsevierarticleCentrifugal microfluidicsLab-on-a-discCentripetal pumpingCheck valvesBiotechnologyTP248.13-248.65ENBiosensors and Bioelectronics: X, Vol 9, Iss , Pp 100085- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Centrifugal microfluidics Lab-on-a-disc Centripetal pumping Check valves Biotechnology TP248.13-248.65
spellingShingle	Centrifugal microfluidics Lab-on-a-disc Centripetal pumping Check valves Biotechnology TP248.13-248.65 David J. Kinahan Robert Burger Daryl Lawlor Philip L. Early Abhishek Vembadi Niamh A. McArdle Niamh A. Kilcawley Macdara T. Glynn Jens Ducrée Centrifugally automated Solid-Phase Extraction of DNA by immiscible liquid valving and chemically powered centripetal pumping of peripherally stored reagents
description	This paper presents two flow-control technologies for use on centrifugal Lab-on-a-Disc systems. The first, immiscible liquid valving, selectively blocks microfluidic channels using a high-density liquid fluorocarbon (FC-40). Used with a specific channel geometry, the FC-40 can permit liquid to enter a chamber but prevents it flowing back along the same path and so acts as “liquid” check-valve. The same liquid can be combined with a water-dissolvable film to provide an extremely robust liquid routing structure. The second technology uses CO2 gas, created by wetting of commodity baking powder by water, to centripetally pump liquid from the periphery of the disc to the centre of the disc. The technologies are combined with valving schemes based on strategically placed, solvent-selective dissolvable films (DFs) to demonstrate repeated pumping of a liquid sample from the edge of the disc to the centre of the disc. The flow-control technologies are then combined to demonstrate fully automated Solid-Phase Extraction (SPE) of DNA with reagent storage on the periphery of the disc. We report an extraction efficiency of 47% measured relative to commercial spin-columns.
format	article
author	David J. Kinahan Robert Burger Daryl Lawlor Philip L. Early Abhishek Vembadi Niamh A. McArdle Niamh A. Kilcawley Macdara T. Glynn Jens Ducrée
author_facet	David J. Kinahan Robert Burger Daryl Lawlor Philip L. Early Abhishek Vembadi Niamh A. McArdle Niamh A. Kilcawley Macdara T. Glynn Jens Ducrée
author_sort	David J. Kinahan
title	Centrifugally automated Solid-Phase Extraction of DNA by immiscible liquid valving and chemically powered centripetal pumping of peripherally stored reagents
title_short	Centrifugally automated Solid-Phase Extraction of DNA by immiscible liquid valving and chemically powered centripetal pumping of peripherally stored reagents
title_full	Centrifugally automated Solid-Phase Extraction of DNA by immiscible liquid valving and chemically powered centripetal pumping of peripherally stored reagents
title_fullStr	Centrifugally automated Solid-Phase Extraction of DNA by immiscible liquid valving and chemically powered centripetal pumping of peripherally stored reagents
title_full_unstemmed	Centrifugally automated Solid-Phase Extraction of DNA by immiscible liquid valving and chemically powered centripetal pumping of peripherally stored reagents
title_sort	centrifugally automated solid-phase extraction of dna by immiscible liquid valving and chemically powered centripetal pumping of peripherally stored reagents
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/f13e5b8f99e149edad5df481a2a6e9b4
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Centrifugally automated Solid-Phase Extraction of DNA by immiscible liquid valving and chemically powered centripetal pumping of peripherally stored reagents

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