Aptamer-Antibody Complementation On Multiwalled Carbon Nanotube-Gold Transduced Dielectrode Surfaces To Detect Pandemic Swine Influenza Virus

Fang Wang,1 Subash CB Gopinath,2,3 Thangavel Lakshmipriya3 1Department of Infectious Diseases,Children’s Hospital Affiliated to Zhengzhou University, Henan Children’s Hospital, Zhengzhou Children’s Hospitality, Zhengzhou 450053, People’s Republic of China; 2Sc...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Wang F, Gopinath SCB, Lakshmipriya T
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	Influenza pandemic Membrane protein Aptasensor Immunosensor Dielectrode sensor Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/f13fef9f2dc84b53bdf3e6f1fb469725
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f13fef9f2dc84b53bdf3e6f1fb469725
record_format	dspace
spelling	oai:doaj.org-article:f13fef9f2dc84b53bdf3e6f1fb4697252021-12-02T09:22:50ZAptamer-Antibody Complementation On Multiwalled Carbon Nanotube-Gold Transduced Dielectrode Surfaces To Detect Pandemic Swine Influenza Virus1178-2013https://doaj.org/article/f13fef9f2dc84b53bdf3e6f1fb4697252019-10-01T00:00:00Zhttps://www.dovepress.com/aptamer-antibody-complementation-on-multiwalled-carbon-nanotube-gold-t-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Fang Wang,1 Subash CB Gopinath,2,3 Thangavel Lakshmipriya3 1Department of Infectious Diseases,Children’s Hospital Affiliated to Zhengzhou University, Henan Children’s Hospital, Zhengzhou Children’s Hospitality, Zhengzhou 450053, People’s Republic of China; 2School of Bioprocess Engineering, Universiti Malaysia Perlis, Arau, Perlis 02600, Malaysia; 3Institute of Nano Electronic Engineering, Universiti Malaysia Perlis, Kangar, Perlis 01000, MalaysiaCorrespondence: Subash CB GopinathSchool of Bioprocess Engineering, Universiti Malaysia Perlis, Arau, Perlis 02600, MalaysiaTel +601110472006Email subash@unimap.edu.myBackground: A pandemic influenza viral strain, influenza A/California/07/2009 (pdmH1N1), has been considered to be a potential issue that needs to be controlled to avoid the seasonal emergence of mutated strains.Materials and methods: In this study, aptamer-antibody complementation was implemented on a multiwalled carbon nanotube-gold conjugated sensing surface with a dielectrode to detect pandemic pdmH1N1. Preliminary biomolecular and dielectrode surface analyses were performed by molecular and microscopic methods. A stable anti-pdmH1N1 aptamer sequence interacted with hemagglutinin (HA) and was compared with the antibody interaction. Both aptamer and antibody attachments on the surface as the basic molecule attained the saturation at nanomolar levels.Results: Aptamers were found to have higher affinity and electric response than antibodies against HA of pdmH1N1. Linear regression with aptamer-HA interaction displays sensitivity in the range of 10 fM, whereas antibody-HA interaction shows a 100-fold lower level (1 pM). When sandwich-based detection of aptamer-HA-antibody and antibody-HA-aptamer was performed, a higher response of current was observed in both cases. Moreover, the detection strategy with aptamer clearly discriminated the closely related HA of influenza B/Tokyo/53/99 and influenza A/Panama/2007/1999 (H3N2).Conclusion: The high performance of the abovementioned detection methods was supported by the apparent specificity and reproducibility by the demonstrated sensing system.Keywords: influenza pandemic, membrane protein, aptasensor, immunosensor, dielectrode sensorWang FGopinath SCBLakshmipriya TDove Medical PressarticleInfluenza pandemicMembrane proteinAptasensorImmunosensorDielectrode sensorMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 8469-8481 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Influenza pandemic Membrane protein Aptasensor Immunosensor Dielectrode sensor Medicine (General) R5-920
spellingShingle	Influenza pandemic Membrane protein Aptasensor Immunosensor Dielectrode sensor Medicine (General) R5-920 Wang F Gopinath SCB Lakshmipriya T Aptamer-Antibody Complementation On Multiwalled Carbon Nanotube-Gold Transduced Dielectrode Surfaces To Detect Pandemic Swine Influenza Virus
description	Fang Wang,1 Subash CB Gopinath,2,3 Thangavel Lakshmipriya3 1Department of Infectious Diseases,Children’s Hospital Affiliated to Zhengzhou University, Henan Children’s Hospital, Zhengzhou Children’s Hospitality, Zhengzhou 450053, People’s Republic of China; 2School of Bioprocess Engineering, Universiti Malaysia Perlis, Arau, Perlis 02600, Malaysia; 3Institute of Nano Electronic Engineering, Universiti Malaysia Perlis, Kangar, Perlis 01000, MalaysiaCorrespondence: Subash CB GopinathSchool of Bioprocess Engineering, Universiti Malaysia Perlis, Arau, Perlis 02600, MalaysiaTel +601110472006Email subash@unimap.edu.myBackground: A pandemic influenza viral strain, influenza A/California/07/2009 (pdmH1N1), has been considered to be a potential issue that needs to be controlled to avoid the seasonal emergence of mutated strains.Materials and methods: In this study, aptamer-antibody complementation was implemented on a multiwalled carbon nanotube-gold conjugated sensing surface with a dielectrode to detect pandemic pdmH1N1. Preliminary biomolecular and dielectrode surface analyses were performed by molecular and microscopic methods. A stable anti-pdmH1N1 aptamer sequence interacted with hemagglutinin (HA) and was compared with the antibody interaction. Both aptamer and antibody attachments on the surface as the basic molecule attained the saturation at nanomolar levels.Results: Aptamers were found to have higher affinity and electric response than antibodies against HA of pdmH1N1. Linear regression with aptamer-HA interaction displays sensitivity in the range of 10 fM, whereas antibody-HA interaction shows a 100-fold lower level (1 pM). When sandwich-based detection of aptamer-HA-antibody and antibody-HA-aptamer was performed, a higher response of current was observed in both cases. Moreover, the detection strategy with aptamer clearly discriminated the closely related HA of influenza B/Tokyo/53/99 and influenza A/Panama/2007/1999 (H3N2).Conclusion: The high performance of the abovementioned detection methods was supported by the apparent specificity and reproducibility by the demonstrated sensing system.Keywords: influenza pandemic, membrane protein, aptasensor, immunosensor, dielectrode sensor
format	article
author	Wang F Gopinath SCB Lakshmipriya T
author_facet	Wang F Gopinath SCB Lakshmipriya T
author_sort	Wang F
title	Aptamer-Antibody Complementation On Multiwalled Carbon Nanotube-Gold Transduced Dielectrode Surfaces To Detect Pandemic Swine Influenza Virus
title_short	Aptamer-Antibody Complementation On Multiwalled Carbon Nanotube-Gold Transduced Dielectrode Surfaces To Detect Pandemic Swine Influenza Virus
title_full	Aptamer-Antibody Complementation On Multiwalled Carbon Nanotube-Gold Transduced Dielectrode Surfaces To Detect Pandemic Swine Influenza Virus
title_fullStr	Aptamer-Antibody Complementation On Multiwalled Carbon Nanotube-Gold Transduced Dielectrode Surfaces To Detect Pandemic Swine Influenza Virus
title_full_unstemmed	Aptamer-Antibody Complementation On Multiwalled Carbon Nanotube-Gold Transduced Dielectrode Surfaces To Detect Pandemic Swine Influenza Virus
title_sort	aptamer-antibody complementation on multiwalled carbon nanotube-gold transduced dielectrode surfaces to detect pandemic swine influenza virus
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/f13fef9f2dc84b53bdf3e6f1fb469725
work_keys_str_mv	AT wangf aptamerantibodycomplementationonmultiwalledcarbonnanotubegoldtransduceddielectrodesurfacestodetectpandemicswineinfluenzavirus AT gopinathscb aptamerantibodycomplementationonmultiwalledcarbonnanotubegoldtransduceddielectrodesurfacestodetectpandemicswineinfluenzavirus AT lakshmipriyat aptamerantibodycomplementationonmultiwalledcarbonnanotubegoldtransduceddielectrodesurfacestodetectpandemicswineinfluenzavirus
_version_	1718398158377058304

Aptamer-Antibody Complementation On Multiwalled Carbon Nanotube-Gold Transduced Dielectrode Surfaces To Detect Pandemic Swine Influenza Virus

Ejemplares similares