A common-path digital holographic microscope with a rotatable birefringent prism for synthesizing high-quality quantitative phase images from multi-angle interferograms
We propose a simple common-path off-axis interferometric system to perform high-quality quantitative phase imaging based on a rotatable birefringent prism that is placed at the output port of a conventional transmission or reflection microscope. The proposed system effectively eliminates the zero-or...
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| Autores principales: | , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Elsevier
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/f142b4cd2edd4d48a66ed6140394aeef |
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| Sumario: | We propose a simple common-path off-axis interferometric system to perform high-quality quantitative phase imaging based on a rotatable birefringent prism that is placed at the output port of a conventional transmission or reflection microscope. The proposed system effectively eliminates the zero-order term of holographic data and increases the phase image reconstruction bandwidth by compounding multi-angle holograms, thereby obtaining high-quality quantitative phase images without zero-order artifacts. In addition, the proposed multi-angle phase image compounding method can eliminate the repetitive object phases caused by self-reference interferometry, thereby alleviating the problem of field-of-view reduction. Furthermore, we realize high-quality quantitative phase imaging based on multi-angle phase images without sacrificing the imaging frame rate. The concept is demonstrated by performing high-quality quantitative phase imaging of polystyrene microspheres and a freshwater alga, Pinnularia. Experimental results show that the noise level of the quantitative phase images synthesized using the proposed rotatable common-path digital holographic microscopy system is lower than that of conventional single-shot digital holographic microscope reconstruction quantitative images, and a more detailed cell internal structure can be observed. |
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