Advances of Glycometabolism Engineering in Chinese Hamster Ovary Cells
As the most widely used mammalian cell line, Chinese hamster ovary (CHO) cells can express various recombinant proteins with a post translational modification pattern similar to that of the proteins from human cells. During industrial production, cells need large amounts of ATP to support growth and...
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Frontiers Media S.A.
2021
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oai:doaj.org-article:f14b438497c949acaf686d8c97bdd03d2021-12-02T10:52:22ZAdvances of Glycometabolism Engineering in Chinese Hamster Ovary Cells2296-418510.3389/fbioe.2021.774175https://doaj.org/article/f14b438497c949acaf686d8c97bdd03d2021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fbioe.2021.774175/fullhttps://doaj.org/toc/2296-4185As the most widely used mammalian cell line, Chinese hamster ovary (CHO) cells can express various recombinant proteins with a post translational modification pattern similar to that of the proteins from human cells. During industrial production, cells need large amounts of ATP to support growth and protein expression, and since glycometabolism is the main source of ATP for cells, protein production partly depends on the efficiency of glycometabolism. And efficient glycometabolism allows less glucose uptake by cells, reducing production costs, and providing a better mammalian production platform for recombinant protein expression. In the present study, a series of progresses on the comprehensive optimization in CHO cells by glycometabolism strategy were reviewed, including carbohydrate intake, pyruvate metabolism and mitochondrial metabolism. We analyzed the effects of gene regulation in the upstream and downstream of the glucose metabolism pathway on cell’s growth and protein expression. And we also pointed out the latest metabolic studies that are potentially applicable on CHO cells. In the end, we elaborated the application of metabolic models in the study of CHO cell metabolism.Huan-Yu ZhangHuan-Yu ZhangZhen-Lin FanZhen-Lin FanTian-Yun WangTian-Yun WangFrontiers Media S.A.articleCHO cellsglycometabolism engineeringpyruvate metabolismaerobic oxidation of glucosemetabolic modelsBiotechnologyTP248.13-248.65ENFrontiers in Bioengineering and Biotechnology, Vol 9 (2021) |
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CHO cells glycometabolism engineering pyruvate metabolism aerobic oxidation of glucose metabolic models Biotechnology TP248.13-248.65 |
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CHO cells glycometabolism engineering pyruvate metabolism aerobic oxidation of glucose metabolic models Biotechnology TP248.13-248.65 Huan-Yu Zhang Huan-Yu Zhang Zhen-Lin Fan Zhen-Lin Fan Tian-Yun Wang Tian-Yun Wang Advances of Glycometabolism Engineering in Chinese Hamster Ovary Cells |
| description |
As the most widely used mammalian cell line, Chinese hamster ovary (CHO) cells can express various recombinant proteins with a post translational modification pattern similar to that of the proteins from human cells. During industrial production, cells need large amounts of ATP to support growth and protein expression, and since glycometabolism is the main source of ATP for cells, protein production partly depends on the efficiency of glycometabolism. And efficient glycometabolism allows less glucose uptake by cells, reducing production costs, and providing a better mammalian production platform for recombinant protein expression. In the present study, a series of progresses on the comprehensive optimization in CHO cells by glycometabolism strategy were reviewed, including carbohydrate intake, pyruvate metabolism and mitochondrial metabolism. We analyzed the effects of gene regulation in the upstream and downstream of the glucose metabolism pathway on cell’s growth and protein expression. And we also pointed out the latest metabolic studies that are potentially applicable on CHO cells. In the end, we elaborated the application of metabolic models in the study of CHO cell metabolism. |
| format |
article |
| author |
Huan-Yu Zhang Huan-Yu Zhang Zhen-Lin Fan Zhen-Lin Fan Tian-Yun Wang Tian-Yun Wang |
| author_facet |
Huan-Yu Zhang Huan-Yu Zhang Zhen-Lin Fan Zhen-Lin Fan Tian-Yun Wang Tian-Yun Wang |
| author_sort |
Huan-Yu Zhang |
| title |
Advances of Glycometabolism Engineering in Chinese Hamster Ovary Cells |
| title_short |
Advances of Glycometabolism Engineering in Chinese Hamster Ovary Cells |
| title_full |
Advances of Glycometabolism Engineering in Chinese Hamster Ovary Cells |
| title_fullStr |
Advances of Glycometabolism Engineering in Chinese Hamster Ovary Cells |
| title_full_unstemmed |
Advances of Glycometabolism Engineering in Chinese Hamster Ovary Cells |
| title_sort |
advances of glycometabolism engineering in chinese hamster ovary cells |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/f14b438497c949acaf686d8c97bdd03d |
| work_keys_str_mv |
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| _version_ |
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