Atomic force microscopy reveals a role for endothelial cell ICAM-1 expression in bladder cancer cell adherence.

Cancer metastasis is a complex process involving cell-cell interactions mediated by cell adhesive molecules. In this study we determine the adhesion strength between an endothelial cell monolayer and tumor cells of different metastatic potentials using Atomic Force Microscopy. We show that the ruptu...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Valérie M Laurent, Alain Duperray, Vinoth Sundar Rajan, Claude Verdier
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f14fbf37482c4bf9bdc0a13e554a1aad
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f14fbf37482c4bf9bdc0a13e554a1aad
record_format	dspace
spelling	oai:doaj.org-article:f14fbf37482c4bf9bdc0a13e554a1aad2021-11-18T08:18:15ZAtomic force microscopy reveals a role for endothelial cell ICAM-1 expression in bladder cancer cell adherence.1932-620310.1371/journal.pone.0098034https://doaj.org/article/f14fbf37482c4bf9bdc0a13e554a1aad2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24857933/?tool=EBIhttps://doaj.org/toc/1932-6203Cancer metastasis is a complex process involving cell-cell interactions mediated by cell adhesive molecules. In this study we determine the adhesion strength between an endothelial cell monolayer and tumor cells of different metastatic potentials using Atomic Force Microscopy. We show that the rupture forces of receptor-ligand bonds increase with retraction speed and range between 20 and 70 pN. It is shown that the most invasive cell lines (T24, J82) form the strongest bonds with endothelial cells. Using ICAM-1 coated substrates and a monoclonal antibody specific for ICAM-1, we demonstrate that ICAM-1 serves as a key receptor on endothelial cells and that its interactions with ligands expressed by tumor cells are correlated with the rupture forces obtained with the most invasive cancer cells (T24, J82). For the less invasive cancer cells (RT112), endothelial ICAM-1 does not seem to play any role in the adhesion process. Moreover, a detailed analysis of the distribution of rupture forces suggests that ICAM-1 interacts preferentially with one ligand on T24 cancer cells and with two ligands on J82 cancer cells. Possible counter receptors for these interactions are CD43 and MUC1, two known ligands for ICAM-1 which are expressed by these cancer cells.Valérie M LaurentAlain DuperrayVinoth Sundar RajanClaude VerdierPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 5, p e98034 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Valérie M Laurent Alain Duperray Vinoth Sundar Rajan Claude Verdier Atomic force microscopy reveals a role for endothelial cell ICAM-1 expression in bladder cancer cell adherence.
description	Cancer metastasis is a complex process involving cell-cell interactions mediated by cell adhesive molecules. In this study we determine the adhesion strength between an endothelial cell monolayer and tumor cells of different metastatic potentials using Atomic Force Microscopy. We show that the rupture forces of receptor-ligand bonds increase with retraction speed and range between 20 and 70 pN. It is shown that the most invasive cell lines (T24, J82) form the strongest bonds with endothelial cells. Using ICAM-1 coated substrates and a monoclonal antibody specific for ICAM-1, we demonstrate that ICAM-1 serves as a key receptor on endothelial cells and that its interactions with ligands expressed by tumor cells are correlated with the rupture forces obtained with the most invasive cancer cells (T24, J82). For the less invasive cancer cells (RT112), endothelial ICAM-1 does not seem to play any role in the adhesion process. Moreover, a detailed analysis of the distribution of rupture forces suggests that ICAM-1 interacts preferentially with one ligand on T24 cancer cells and with two ligands on J82 cancer cells. Possible counter receptors for these interactions are CD43 and MUC1, two known ligands for ICAM-1 which are expressed by these cancer cells.
format	article
author	Valérie M Laurent Alain Duperray Vinoth Sundar Rajan Claude Verdier
author_facet	Valérie M Laurent Alain Duperray Vinoth Sundar Rajan Claude Verdier
author_sort	Valérie M Laurent
title	Atomic force microscopy reveals a role for endothelial cell ICAM-1 expression in bladder cancer cell adherence.
title_short	Atomic force microscopy reveals a role for endothelial cell ICAM-1 expression in bladder cancer cell adherence.
title_full	Atomic force microscopy reveals a role for endothelial cell ICAM-1 expression in bladder cancer cell adherence.
title_fullStr	Atomic force microscopy reveals a role for endothelial cell ICAM-1 expression in bladder cancer cell adherence.
title_full_unstemmed	Atomic force microscopy reveals a role for endothelial cell ICAM-1 expression in bladder cancer cell adherence.
title_sort	atomic force microscopy reveals a role for endothelial cell icam-1 expression in bladder cancer cell adherence.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/f14fbf37482c4bf9bdc0a13e554a1aad
work_keys_str_mv	AT valeriemlaurent atomicforcemicroscopyrevealsaroleforendothelialcellicam1expressioninbladdercancercelladherence AT alainduperray atomicforcemicroscopyrevealsaroleforendothelialcellicam1expressioninbladdercancercelladherence AT vinothsundarrajan atomicforcemicroscopyrevealsaroleforendothelialcellicam1expressioninbladdercancercelladherence AT claudeverdier atomicforcemicroscopyrevealsaroleforendothelialcellicam1expressioninbladdercancercelladherence
_version_	1718421959223541760

Atomic force microscopy reveals a role for endothelial cell ICAM-1 expression in bladder cancer cell adherence.

Ejemplares similares