Metabolic imaging of energy metabolism in traumatic brain injury using hyperpolarized [1-13C]pyruvate

Abstract Traumatic brain injury (TBI) is known to cause perturbations in the energy metabolism of the brain, but current tests of metabolic activity are only indirect markers of energy use or are highly invasive. Here we show that hyperpolarized 13C magnetic resonance spectroscopic imaging (MRSI) ca...

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Autores principales:	Stephen J. DeVience, Xin Lu, Julie Proctor, Parisa Rangghran, Elias R. Melhem, Rao Gullapalli, Gary M. Fiskum, Dirk Mayer
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f150a3658e674458a589a11117522d4d
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spelling	oai:doaj.org-article:f150a3658e674458a589a11117522d4d2021-12-02T12:31:59ZMetabolic imaging of energy metabolism in traumatic brain injury using hyperpolarized [1-13C]pyruvate10.1038/s41598-017-01736-x2045-2322https://doaj.org/article/f150a3658e674458a589a11117522d4d2017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01736-xhttps://doaj.org/toc/2045-2322Abstract Traumatic brain injury (TBI) is known to cause perturbations in the energy metabolism of the brain, but current tests of metabolic activity are only indirect markers of energy use or are highly invasive. Here we show that hyperpolarized 13C magnetic resonance spectroscopic imaging (MRSI) can be used as a direct, non-invasive method for studying the effects of TBI on energy metabolism. Measurements were performed on rats with moderate TBI induced by controlled cortical impact on one cerebral hemisphere. Following injection of hyperpolarized [1-13C]pyruvate, the resulting 13C-bicarbonate signal was found to be 24 ± 6% lower in the injured hemisphere compared with the non-injured hemisphere, while the hyperpolarized bicarbonate-to-lactate ratio was 33 ± 8% lower in the injured hemisphere. In a control group, no significant difference in signal was found between sides of the brain. The results suggest an impairment in mitochondrial pyruvate metabolism, resulting in a decrease in aerobic respiration at the location of injury following TBI.Stephen J. DeVienceXin LuJulie ProctorParisa RangghranElias R. MelhemRao GullapalliGary M. FiskumDirk MayerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-7 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Stephen J. DeVience Xin Lu Julie Proctor Parisa Rangghran Elias R. Melhem Rao Gullapalli Gary M. Fiskum Dirk Mayer Metabolic imaging of energy metabolism in traumatic brain injury using hyperpolarized [1-13C]pyruvate
description	Abstract Traumatic brain injury (TBI) is known to cause perturbations in the energy metabolism of the brain, but current tests of metabolic activity are only indirect markers of energy use or are highly invasive. Here we show that hyperpolarized 13C magnetic resonance spectroscopic imaging (MRSI) can be used as a direct, non-invasive method for studying the effects of TBI on energy metabolism. Measurements were performed on rats with moderate TBI induced by controlled cortical impact on one cerebral hemisphere. Following injection of hyperpolarized [1-13C]pyruvate, the resulting 13C-bicarbonate signal was found to be 24 ± 6% lower in the injured hemisphere compared with the non-injured hemisphere, while the hyperpolarized bicarbonate-to-lactate ratio was 33 ± 8% lower in the injured hemisphere. In a control group, no significant difference in signal was found between sides of the brain. The results suggest an impairment in mitochondrial pyruvate metabolism, resulting in a decrease in aerobic respiration at the location of injury following TBI.
format	article
author	Stephen J. DeVience Xin Lu Julie Proctor Parisa Rangghran Elias R. Melhem Rao Gullapalli Gary M. Fiskum Dirk Mayer
author_facet	Stephen J. DeVience Xin Lu Julie Proctor Parisa Rangghran Elias R. Melhem Rao Gullapalli Gary M. Fiskum Dirk Mayer
author_sort	Stephen J. DeVience
title	Metabolic imaging of energy metabolism in traumatic brain injury using hyperpolarized [1-13C]pyruvate
title_short	Metabolic imaging of energy metabolism in traumatic brain injury using hyperpolarized [1-13C]pyruvate
title_full	Metabolic imaging of energy metabolism in traumatic brain injury using hyperpolarized [1-13C]pyruvate
title_fullStr	Metabolic imaging of energy metabolism in traumatic brain injury using hyperpolarized [1-13C]pyruvate
title_full_unstemmed	Metabolic imaging of energy metabolism in traumatic brain injury using hyperpolarized [1-13C]pyruvate
title_sort	metabolic imaging of energy metabolism in traumatic brain injury using hyperpolarized [1-13c]pyruvate
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/f150a3658e674458a589a11117522d4d
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Metabolic imaging of energy metabolism in traumatic brain injury using hyperpolarized [1-13C]pyruvate

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