A Staphylococcus aureus small RNA is required for bacterial virulence and regulates the expression of an immune-evasion molecule.

Staphylococcus aureus, a pathogen responsible for hospital and community-acquired infections, expresses many virulence factors under the control of numerous regulatory systems. Here we show that one of the small pathogenicity island RNAs, named SprD, contributes significantly to causing disease in a...

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Autores principales:	Svetlana Chabelskaya, Olivier Gaillot, Brice Felden
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/f1543173c3bb48889fcad18bc43821e7
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spelling	oai:doaj.org-article:f1543173c3bb48889fcad18bc43821e72021-12-02T20:00:36ZA Staphylococcus aureus small RNA is required for bacterial virulence and regulates the expression of an immune-evasion molecule.1553-73661553-737410.1371/journal.ppat.1000927https://doaj.org/article/f1543173c3bb48889fcad18bc43821e72010-06-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20532214/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Staphylococcus aureus, a pathogen responsible for hospital and community-acquired infections, expresses many virulence factors under the control of numerous regulatory systems. Here we show that one of the small pathogenicity island RNAs, named SprD, contributes significantly to causing disease in an animal model of infection. We have identified one of the targets of SprD and our in vivo data demonstrate that SprD negatively regulates the expression of the Sbi immune-evasion molecule, impairing both the adaptive and innate host immune responses. SprD interacts with the 5' part of the sbi mRNA and structural mapping of SprD, its mRNA target, and the 'SprD-mRNA' duplex, in combination with mutational analysis, reveals the molecular details of the regulation. It demonstrates that the accessible SprD central region interacts with the sbi mRNA translational start site. We show by toeprint experiments that SprD prevents translation initiation of sbi mRNA by an antisense mechanism. SprD is a small regulatory RNA required for S. aureus pathogenicity with an identified function, although the mechanism of virulence control by the RNA is yet to be elucidated.Svetlana ChabelskayaOlivier GaillotBrice FeldenPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 6, Iss 6, p e1000927 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Svetlana Chabelskaya Olivier Gaillot Brice Felden A Staphylococcus aureus small RNA is required for bacterial virulence and regulates the expression of an immune-evasion molecule.
description	Staphylococcus aureus, a pathogen responsible for hospital and community-acquired infections, expresses many virulence factors under the control of numerous regulatory systems. Here we show that one of the small pathogenicity island RNAs, named SprD, contributes significantly to causing disease in an animal model of infection. We have identified one of the targets of SprD and our in vivo data demonstrate that SprD negatively regulates the expression of the Sbi immune-evasion molecule, impairing both the adaptive and innate host immune responses. SprD interacts with the 5' part of the sbi mRNA and structural mapping of SprD, its mRNA target, and the 'SprD-mRNA' duplex, in combination with mutational analysis, reveals the molecular details of the regulation. It demonstrates that the accessible SprD central region interacts with the sbi mRNA translational start site. We show by toeprint experiments that SprD prevents translation initiation of sbi mRNA by an antisense mechanism. SprD is a small regulatory RNA required for S. aureus pathogenicity with an identified function, although the mechanism of virulence control by the RNA is yet to be elucidated.
format	article
author	Svetlana Chabelskaya Olivier Gaillot Brice Felden
author_facet	Svetlana Chabelskaya Olivier Gaillot Brice Felden
author_sort	Svetlana Chabelskaya
title	A Staphylococcus aureus small RNA is required for bacterial virulence and regulates the expression of an immune-evasion molecule.
title_short	A Staphylococcus aureus small RNA is required for bacterial virulence and regulates the expression of an immune-evasion molecule.
title_full	A Staphylococcus aureus small RNA is required for bacterial virulence and regulates the expression of an immune-evasion molecule.
title_fullStr	A Staphylococcus aureus small RNA is required for bacterial virulence and regulates the expression of an immune-evasion molecule.
title_full_unstemmed	A Staphylococcus aureus small RNA is required for bacterial virulence and regulates the expression of an immune-evasion molecule.
title_sort	staphylococcus aureus small rna is required for bacterial virulence and regulates the expression of an immune-evasion molecule.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/f1543173c3bb48889fcad18bc43821e7
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A Staphylococcus aureus small RNA is required for bacterial virulence and regulates the expression of an immune-evasion molecule.

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