Vitamin D receptor rs7975232, rs731236 and rs1544410 single nucleotide polymorphisms, and 25-hydroxyvitamin D levels in Egyptian children with type 1 diabetes mellitus: effect of vitamin D co-therapy

Ahmed El-Abd Ahmed,1 Hala M Sakhr,1 Mohammed H Hassan,2 Mostafa I El-Amir,3 Hesham H Ameen41Department of Pediatrics, Faculty of Medicine, South Valley University, Qena, Egypt; 2Department of Medical Biochemistry, Faculty of Medicine, South Valley University, Qena, Egypt; 3Department of Medical Micr...

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Autores principales:	Ahmed AEA, Sakhr HM, Hassan MH, El-Amir MI, Ameen HH
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	Vitamin D receptor single nucleotide polymorphisms Genomic DNA Vitamin D type 1 diabetes mellitus Egyptian children Vitamin D therapy. Specialties of internal medicine RC581-951
Acceso en línea:	https://doaj.org/article/f15698559f854683bcf9453f8c080764
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spelling	oai:doaj.org-article:f15698559f854683bcf9453f8c0807642021-12-02T05:50:19ZVitamin D receptor rs7975232, rs731236 and rs1544410 single nucleotide polymorphisms, and 25-hydroxyvitamin D levels in Egyptian children with type 1 diabetes mellitus: effect of vitamin D co-therapy1178-7007https://doaj.org/article/f15698559f854683bcf9453f8c0807642019-05-01T00:00:00Zhttps://www.dovepress.com/vitamin-d-receptor-rs7975232-rs731236-and-rs1544410-single-nucleotide--peer-reviewed-article-DMSOhttps://doaj.org/toc/1178-7007Ahmed El-Abd Ahmed,1 Hala M Sakhr,1 Mohammed H Hassan,2 Mostafa I El-Amir,3 Hesham H Ameen41Department of Pediatrics, Faculty of Medicine, South Valley University, Qena, Egypt; 2Department of Medical Biochemistry, Faculty of Medicine, South Valley University, Qena, Egypt; 3Department of Medical Microbiology and Immunology, Faculty of Medicine, South Valley University, Qena, Egypt; 4Department of Clinical Pathology, Faculty of Medicine, Al-Azhar University (Assiut Branch), Assiut, EgyptPurpose: We aimed to examine the possible association role of vitamin D and vitamin D receptor (VDR) single nucleotide polymorphisms (SNPs) in type 1 diabetes mellitus (T1DM) development, glycemic control and complications among a cohort of Egyptian children.Subjects and methods: A prospective case-control study has been conducted on 50 Egyptian children with T1DM who were comparable with 50 controls. Vitamin D and HbA1c were measured. VDR-SNPs [ApaI (rs7975232), TaqI (rs731236) and BsmI (rs1544410)] detection was done by polymerase chain reaction through restriction fragment length polymorphism (PCR-RFLP) technique. Vitamin D supplements were given to the included T1DM children with low vitamin D and reassessments of both HbA1c% and 25(OH)D serum levels were performed in those children three months later.Results: Eighty percent of the included diabetic patients have poor glycemic control. Vitamin D was deficient in 68% and insufficient in 16% of diabetic patients. Significant improvements in both vitamin D and glycemic status among T1DM children, who have low vitamin D and received vitamin D supplementations. There were significantly negative correlations between serum levels of vitamin D with both HbA1c % (r= –0.358, P˂0.05) and daily insulin dose (r=−0.473, P˂0.05). Compared with controls, T1DM children presented more commonly with ApaI a allele (OR: 2.87; 95%CI: 1.39–5.91, P˂0.05) and BsmI b allele (OR: 4.38; 95%CI: 2.30–8.33, P˂0.05). TaqI t allele wasn’t significantly differing among patients and controls (P˃0.05). Aa+aa and Bb+bb genotypes were significantly higher among T1DM vs the controls (OR: 3.08;, 95%CI: 1.33–7.15, P˂0.05 and OR: 9.33; 95%CI: 3.61–24.17, P˂0.05respectively).Conclusion: ApaI and BsmI were associated with risk of T1DM development among Egyptian children. Low vitamin D status was frequently occurring among T1DM with significant improvement in the glycemic control of such children when adding vitamin D supplements to the standard insulin therapy.Keywords: vitamin D receptor single nucleotide polymorphisms, genomic DNA, vitamin D, type 1 diabetes mellitus, Egyptian children, vitamin D therapyAhmed AEASakhr HMHassan MHEl-Amir MIAmeen HHDove Medical PressarticleVitamin D receptor single nucleotide polymorphismsGenomic DNAVitamin Dtype 1 diabetes mellitusEgyptian childrenVitamin D therapy.Specialties of internal medicineRC581-951ENDiabetes, Metabolic Syndrome and Obesity: Targets and Therapy, Vol Volume 12, Pp 703-716 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Vitamin D receptor single nucleotide polymorphisms Genomic DNA Vitamin D type 1 diabetes mellitus Egyptian children Vitamin D therapy. Specialties of internal medicine RC581-951
spellingShingle	Vitamin D receptor single nucleotide polymorphisms Genomic DNA Vitamin D type 1 diabetes mellitus Egyptian children Vitamin D therapy. Specialties of internal medicine RC581-951 Ahmed AEA Sakhr HM Hassan MH El-Amir MI Ameen HH Vitamin D receptor rs7975232, rs731236 and rs1544410 single nucleotide polymorphisms, and 25-hydroxyvitamin D levels in Egyptian children with type 1 diabetes mellitus: effect of vitamin D co-therapy
description	Ahmed El-Abd Ahmed,1 Hala M Sakhr,1 Mohammed H Hassan,2 Mostafa I El-Amir,3 Hesham H Ameen41Department of Pediatrics, Faculty of Medicine, South Valley University, Qena, Egypt; 2Department of Medical Biochemistry, Faculty of Medicine, South Valley University, Qena, Egypt; 3Department of Medical Microbiology and Immunology, Faculty of Medicine, South Valley University, Qena, Egypt; 4Department of Clinical Pathology, Faculty of Medicine, Al-Azhar University (Assiut Branch), Assiut, EgyptPurpose: We aimed to examine the possible association role of vitamin D and vitamin D receptor (VDR) single nucleotide polymorphisms (SNPs) in type 1 diabetes mellitus (T1DM) development, glycemic control and complications among a cohort of Egyptian children.Subjects and methods: A prospective case-control study has been conducted on 50 Egyptian children with T1DM who were comparable with 50 controls. Vitamin D and HbA1c were measured. VDR-SNPs [ApaI (rs7975232), TaqI (rs731236) and BsmI (rs1544410)] detection was done by polymerase chain reaction through restriction fragment length polymorphism (PCR-RFLP) technique. Vitamin D supplements were given to the included T1DM children with low vitamin D and reassessments of both HbA1c% and 25(OH)D serum levels were performed in those children three months later.Results: Eighty percent of the included diabetic patients have poor glycemic control. Vitamin D was deficient in 68% and insufficient in 16% of diabetic patients. Significant improvements in both vitamin D and glycemic status among T1DM children, who have low vitamin D and received vitamin D supplementations. There were significantly negative correlations between serum levels of vitamin D with both HbA1c % (r= –0.358, P˂0.05) and daily insulin dose (r=−0.473, P˂0.05). Compared with controls, T1DM children presented more commonly with ApaI a allele (OR: 2.87; 95%CI: 1.39–5.91, P˂0.05) and BsmI b allele (OR: 4.38; 95%CI: 2.30–8.33, P˂0.05). TaqI t allele wasn’t significantly differing among patients and controls (P˃0.05). Aa+aa and Bb+bb genotypes were significantly higher among T1DM vs the controls (OR: 3.08;, 95%CI: 1.33–7.15, P˂0.05 and OR: 9.33; 95%CI: 3.61–24.17, P˂0.05respectively).Conclusion: ApaI and BsmI were associated with risk of T1DM development among Egyptian children. Low vitamin D status was frequently occurring among T1DM with significant improvement in the glycemic control of such children when adding vitamin D supplements to the standard insulin therapy.Keywords: vitamin D receptor single nucleotide polymorphisms, genomic DNA, vitamin D, type 1 diabetes mellitus, Egyptian children, vitamin D therapy
format	article
author	Ahmed AEA Sakhr HM Hassan MH El-Amir MI Ameen HH
author_facet	Ahmed AEA Sakhr HM Hassan MH El-Amir MI Ameen HH
author_sort	Ahmed AEA
title	Vitamin D receptor rs7975232, rs731236 and rs1544410 single nucleotide polymorphisms, and 25-hydroxyvitamin D levels in Egyptian children with type 1 diabetes mellitus: effect of vitamin D co-therapy
title_short	Vitamin D receptor rs7975232, rs731236 and rs1544410 single nucleotide polymorphisms, and 25-hydroxyvitamin D levels in Egyptian children with type 1 diabetes mellitus: effect of vitamin D co-therapy
title_full	Vitamin D receptor rs7975232, rs731236 and rs1544410 single nucleotide polymorphisms, and 25-hydroxyvitamin D levels in Egyptian children with type 1 diabetes mellitus: effect of vitamin D co-therapy
title_fullStr	Vitamin D receptor rs7975232, rs731236 and rs1544410 single nucleotide polymorphisms, and 25-hydroxyvitamin D levels in Egyptian children with type 1 diabetes mellitus: effect of vitamin D co-therapy
title_full_unstemmed	Vitamin D receptor rs7975232, rs731236 and rs1544410 single nucleotide polymorphisms, and 25-hydroxyvitamin D levels in Egyptian children with type 1 diabetes mellitus: effect of vitamin D co-therapy
title_sort	vitamin d receptor rs7975232, rs731236 and rs1544410 single nucleotide polymorphisms, and 25-hydroxyvitamin d levels in egyptian children with type 1 diabetes mellitus: effect of vitamin d co-therapy
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/f15698559f854683bcf9453f8c080764
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Vitamin D receptor rs7975232, rs731236 and rs1544410 single nucleotide polymorphisms, and 25-hydroxyvitamin D levels in Egyptian children with type 1 diabetes mellitus: effect of vitamin D co-therapy

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