Integrated Transcriptomics and Metabolomics Analysis Reveal Key Metabolism Pathways Contributing to Cold Tolerance in Peanut

Low temperature (non-freezing) is one of the major limiting factors in peanut (Arachis hypogaea L.) growth, yield, and geographic distribution. Due to the complexity of cold-resistance trait in peanut, the molecular mechanism of cold tolerance and related gene networks were largely unknown. In this...

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Autores principales:	Xin Wang, Yue Liu, Zhongkui Han, Yuning Chen, Dongxin Huai, Yanping Kang, Zhihui Wang, Liying Yan, Huifang Jiang, Yong Lei, Boshou Liao
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Arachis hypogaea RNA-Seq metabolome carbohydrate polyamine lignin Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/f15b0651c1934e4a876879c355bd5554
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spelling	oai:doaj.org-article:f15b0651c1934e4a876879c355bd55542021-11-30T19:15:17ZIntegrated Transcriptomics and Metabolomics Analysis Reveal Key Metabolism Pathways Contributing to Cold Tolerance in Peanut1664-462X10.3389/fpls.2021.752474https://doaj.org/article/f15b0651c1934e4a876879c355bd55542021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.752474/fullhttps://doaj.org/toc/1664-462XLow temperature (non-freezing) is one of the major limiting factors in peanut (Arachis hypogaea L.) growth, yield, and geographic distribution. Due to the complexity of cold-resistance trait in peanut, the molecular mechanism of cold tolerance and related gene networks were largely unknown. In this study, metabolomic analysis of two peanut cultivars subjected to chilling stress obtained a set of cold-responsive metabolites, including several carbohydrates and polyamines. These substances showed a higher accumulation pattern in cold-tolerant variety SLH than cold-susceptible variety ZH12 under cold stress, indicating their importance in protecting peanut from chilling injuries. In addition, 3,620 cold tolerance genes (CTGs) were identified by transcriptome sequencing, and the CTGs were most significantly enriched in the “phenylpropanoid biosynthesis” pathway. Two vital modules and several novel hub genes were obtained by weighted gene co-expression network analysis (WGCNA). Several key genes involved in soluble sugar, polyamine, and G-lignin biosynthetic pathways were substantially higher and/or responded more quickly in SLH (cold tolerant) than ZH12 (cold susceptible) under low temperature, suggesting they might be crucial contributors during the adaptation of peanut to low temperature. These findings will not only provide valuable resources for study of cold resistance in peanut but also lay a foundation for genetic modification of cold regulators to enhance stress tolerance in crops.Xin WangYue LiuZhongkui HanYuning ChenDongxin HuaiYanping KangZhihui WangLiying YanHuifang JiangYong LeiBoshou LiaoFrontiers Media S.A.articleArachis hypogaeaRNA-SeqmetabolomecarbohydratepolyamineligninPlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Arachis hypogaea RNA-Seq metabolome carbohydrate polyamine lignin Plant culture SB1-1110
spellingShingle	Arachis hypogaea RNA-Seq metabolome carbohydrate polyamine lignin Plant culture SB1-1110 Xin Wang Yue Liu Zhongkui Han Yuning Chen Dongxin Huai Yanping Kang Zhihui Wang Liying Yan Huifang Jiang Yong Lei Boshou Liao Integrated Transcriptomics and Metabolomics Analysis Reveal Key Metabolism Pathways Contributing to Cold Tolerance in Peanut
description	Low temperature (non-freezing) is one of the major limiting factors in peanut (Arachis hypogaea L.) growth, yield, and geographic distribution. Due to the complexity of cold-resistance trait in peanut, the molecular mechanism of cold tolerance and related gene networks were largely unknown. In this study, metabolomic analysis of two peanut cultivars subjected to chilling stress obtained a set of cold-responsive metabolites, including several carbohydrates and polyamines. These substances showed a higher accumulation pattern in cold-tolerant variety SLH than cold-susceptible variety ZH12 under cold stress, indicating their importance in protecting peanut from chilling injuries. In addition, 3,620 cold tolerance genes (CTGs) were identified by transcriptome sequencing, and the CTGs were most significantly enriched in the “phenylpropanoid biosynthesis” pathway. Two vital modules and several novel hub genes were obtained by weighted gene co-expression network analysis (WGCNA). Several key genes involved in soluble sugar, polyamine, and G-lignin biosynthetic pathways were substantially higher and/or responded more quickly in SLH (cold tolerant) than ZH12 (cold susceptible) under low temperature, suggesting they might be crucial contributors during the adaptation of peanut to low temperature. These findings will not only provide valuable resources for study of cold resistance in peanut but also lay a foundation for genetic modification of cold regulators to enhance stress tolerance in crops.
format	article
author	Xin Wang Yue Liu Zhongkui Han Yuning Chen Dongxin Huai Yanping Kang Zhihui Wang Liying Yan Huifang Jiang Yong Lei Boshou Liao
author_facet	Xin Wang Yue Liu Zhongkui Han Yuning Chen Dongxin Huai Yanping Kang Zhihui Wang Liying Yan Huifang Jiang Yong Lei Boshou Liao
author_sort	Xin Wang
title	Integrated Transcriptomics and Metabolomics Analysis Reveal Key Metabolism Pathways Contributing to Cold Tolerance in Peanut
title_short	Integrated Transcriptomics and Metabolomics Analysis Reveal Key Metabolism Pathways Contributing to Cold Tolerance in Peanut
title_full	Integrated Transcriptomics and Metabolomics Analysis Reveal Key Metabolism Pathways Contributing to Cold Tolerance in Peanut
title_fullStr	Integrated Transcriptomics and Metabolomics Analysis Reveal Key Metabolism Pathways Contributing to Cold Tolerance in Peanut
title_full_unstemmed	Integrated Transcriptomics and Metabolomics Analysis Reveal Key Metabolism Pathways Contributing to Cold Tolerance in Peanut
title_sort	integrated transcriptomics and metabolomics analysis reveal key metabolism pathways contributing to cold tolerance in peanut
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/f15b0651c1934e4a876879c355bd5554
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Integrated Transcriptomics and Metabolomics Analysis Reveal Key Metabolism Pathways Contributing to Cold Tolerance in Peanut

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