The use of dopamine-hyaluronate associate-coated maghemite nanoparticles to label cells

Michal Babic1,2, Daniel Horak1,2, Pavla Jendelova2,3, Vit Herynek2,4, Vladimir Proks1,2, Vaclav Vanecek2,3, Petr Lesny2,3, Eva Sykova2,31Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovsky Sq 2, 2Center for Cell Therapy and Tissue Repair, Charles University, V...

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Autores principales:	Babic M, Horak D, Jendelova P, Herynek V, Proks V, Vanecek V, Lesny P, Sykova E
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/f17069b23e224289b691d9a27a6a0c42
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spelling	oai:doaj.org-article:f17069b23e224289b691d9a27a6a0c422021-12-02T00:22:13ZThe use of dopamine-hyaluronate associate-coated maghemite nanoparticles to label cells1176-91141178-2013https://doaj.org/article/f17069b23e224289b691d9a27a6a0c422012-03-01T00:00:00Zhttp://www.dovepress.com/the-use-of-dopamine-hyaluronate-associate-coated-maghemite-nanoparticl-a9511https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Michal Babic1,2, Daniel Horak1,2, Pavla Jendelova2,3, Vit Herynek2,4, Vladimir Proks1,2, Vaclav Vanecek2,3, Petr Lesny2,3, Eva Sykova2,31Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovsky Sq 2, 2Center for Cell Therapy and Tissue Repair, Charles University, V Uvalu 84, 3Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Videnska 1083, 4Institute for Clinical and Experimental Medicine, Videnska 1958, Prague, Czech RepublicAbstract: Sodium hyaluronate (HA) was associated with dopamine (DPA) and introduced as a coating for maghemite (&gamma;-Fe2O3) nanoparticles obtained by the coprecipitation of iron(II) and iron(III) chlorides and oxidation with sodium hypochlorite. The effects of the DPA anchorage of HA on the &gamma;-Fe2O3 surface on the physicochemical properties of the resulting colloids were investigated. Nanoparticles coated at three different DPA-HA/&gamma;-Fe2O3 and DPA/HA ratios were chosen for experiments with rat bone marrow mesenchymal stem cells and human chondrocytes. The nanoparticles were internalized into rat bone marrow mesenchymal stem cells via endocytosis as confirmed by Prussian Blue staining. The efficiency of mesenchymal stem cell labeling was analyzed. From among the investigated samples, efficient cell labeling was achieved by using DPA-HA-&gamma;-Fe2O3 nanoparticles with DPA-HA/&gamma;-Fe2O3 = 0.45 (weight/weight) and DPA/HA = 0.038 (weight/weight) ratios. The particles were used as a contrast agent in magnetic resonance imaging for the labeling and visualization of cells.Keywords: nanoparticles, dopamine, hyaluronate, cell labeling, magneticBabic MHorak DJendelova PHerynek VProks VVanecek VLesny PSykova EDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 1461-1474 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Babic M Horak D Jendelova P Herynek V Proks V Vanecek V Lesny P Sykova E The use of dopamine-hyaluronate associate-coated maghemite nanoparticles to label cells
description	Michal Babic1,2, Daniel Horak1,2, Pavla Jendelova2,3, Vit Herynek2,4, Vladimir Proks1,2, Vaclav Vanecek2,3, Petr Lesny2,3, Eva Sykova2,31Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovsky Sq 2, 2Center for Cell Therapy and Tissue Repair, Charles University, V Uvalu 84, 3Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Videnska 1083, 4Institute for Clinical and Experimental Medicine, Videnska 1958, Prague, Czech RepublicAbstract: Sodium hyaluronate (HA) was associated with dopamine (DPA) and introduced as a coating for maghemite (&gamma;-Fe2O3) nanoparticles obtained by the coprecipitation of iron(II) and iron(III) chlorides and oxidation with sodium hypochlorite. The effects of the DPA anchorage of HA on the &gamma;-Fe2O3 surface on the physicochemical properties of the resulting colloids were investigated. Nanoparticles coated at three different DPA-HA/&gamma;-Fe2O3 and DPA/HA ratios were chosen for experiments with rat bone marrow mesenchymal stem cells and human chondrocytes. The nanoparticles were internalized into rat bone marrow mesenchymal stem cells via endocytosis as confirmed by Prussian Blue staining. The efficiency of mesenchymal stem cell labeling was analyzed. From among the investigated samples, efficient cell labeling was achieved by using DPA-HA-&gamma;-Fe2O3 nanoparticles with DPA-HA/&gamma;-Fe2O3 = 0.45 (weight/weight) and DPA/HA = 0.038 (weight/weight) ratios. The particles were used as a contrast agent in magnetic resonance imaging for the labeling and visualization of cells.Keywords: nanoparticles, dopamine, hyaluronate, cell labeling, magnetic
format	article
author	Babic M Horak D Jendelova P Herynek V Proks V Vanecek V Lesny P Sykova E
author_facet	Babic M Horak D Jendelova P Herynek V Proks V Vanecek V Lesny P Sykova E
author_sort	Babic M
title	The use of dopamine-hyaluronate associate-coated maghemite nanoparticles to label cells
title_short	The use of dopamine-hyaluronate associate-coated maghemite nanoparticles to label cells
title_full	The use of dopamine-hyaluronate associate-coated maghemite nanoparticles to label cells
title_fullStr	The use of dopamine-hyaluronate associate-coated maghemite nanoparticles to label cells
title_full_unstemmed	The use of dopamine-hyaluronate associate-coated maghemite nanoparticles to label cells
title_sort	use of dopamine-hyaluronate associate-coated maghemite nanoparticles to label cells
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/f17069b23e224289b691d9a27a6a0c42
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The use of dopamine-hyaluronate associate-coated maghemite nanoparticles to label cells

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