The Renoprotective Effects of Naringenin (NGN) in Gestational Pregnancy

Chunrong Zhao,1 Xiufeng Yin,2 Chunping Zhao1 1Department of Obstetrics, Linyi Central Hospital of Shandong Province, Linyi, Shandong 276400, People’s Republic of China; 2Department of Obstetrics and Gynecology, Yixing People’s Hospital, Wuxi, Jiangsu 214200, People’s Re...

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Autores principales:	Zhao C, Yin X
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	gestational diabetes mellitus naringenin streptozotocin oxidative stress inflammation Specialties of internal medicine RC581-951
Acceso en línea:	https://doaj.org/article/f1786c955658488ea0d097eed7ec9045
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spelling	oai:doaj.org-article:f1786c955658488ea0d097eed7ec90452021-12-02T09:16:00ZThe Renoprotective Effects of Naringenin (NGN) in Gestational Pregnancy1178-7007https://doaj.org/article/f1786c955658488ea0d097eed7ec90452020-01-01T00:00:00Zhttps://www.dovepress.com/the-renoprotective-effects-of-naringenin-ngn-in-gestational-pregnancy-peer-reviewed-article-DMSOhttps://doaj.org/toc/1178-7007Chunrong Zhao,1 Xiufeng Yin,2 Chunping Zhao1 1Department of Obstetrics, Linyi Central Hospital of Shandong Province, Linyi, Shandong 276400, People’s Republic of China; 2Department of Obstetrics and Gynecology, Yixing People’s Hospital, Wuxi, Jiangsu 214200, People’s Republic of ChinaCorrespondence: Xiufeng YinDepartment of Obstetrics and Gynecology, Yixing People’s Hospital, No. 75 Tongzhen Guan Road, Yicheng, Yixing, Jiangsu 214200, People’s Republic of ChinaTel +86-510-8733 0741Email yin393439460@163.comIntroduction: Gestational diabetes mellitus (GDM) is defined as glucose intolerance that is first diagnosed during pregnancy, a condition risking the health of both the mother and the baby. Naringenin (NGN) has been demonstrated to have multiple therapeutic functions, while it is also considered to exhibit antidiabetic properties. The present study aimed to investigate the protective effects of NGN in pregnant diabetic rats.Methods: GDM was induced by feeding the rats with a high-fat diet for 5 weeks, followed by intraperitoneal injection of streptozotocin (35 mg/kg). The fasting blood glucose were determined with a glucometer and the 24-h urine protein (24-UPro) were determined by the sulfonyl salicylic acid method. The pathological morphological changes and apoptosis of glomeruli cells of kidney tissue using hematoxylin and eosin (H&E) staining and TUNEL analysis. Enzyme-linked immunosorbent assay (ELISA) kits were used to detect the serum T-AOC, the activity of SOD, the levels of GSH-Px, CAT and MDA, TNF-α, IL-6, TGF-β, ICAM-1.The expression of related genes were measured by RT-qPCR and Western blot analyses.Results: In the NGN-treated group, it was observed that the general status of the rats was improved, while the levels of blood glucose and 24-UPro were significantly decreased. In addition, the histopathological changes in renal tissues and renal cell apoptosis were significantly improved upon treatment with NGN. The expression levels of oxidative stress and inflammation-associated factors also differed signifigcantly between the model and NGN-treated groups. Upon treatment with NGN, the levels of peroxisome proliferator-activated receptor α were significantly increased, while the activity of enzymes involved in the oxidative metabolism of fatty acids was significantly decreased.Conclusion: These preliminary experimental findings demonstrate that NGN has a certain renoprotective effect on GDM, which provides a novel therapeutic option for this condition.Keywords: gestational diabetes mellitus, Naringenin, streptozotocin, oxidative stress, inflammationZhao CYin XZhao CDove Medical Pressarticlegestational diabetes mellitusnaringeninstreptozotocinoxidative stressinflammationSpecialties of internal medicineRC581-951ENDiabetes, Metabolic Syndrome and Obesity: Targets and Therapy, Vol Volume 13, Pp 53-63 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	gestational diabetes mellitus naringenin streptozotocin oxidative stress inflammation Specialties of internal medicine RC581-951
spellingShingle	gestational diabetes mellitus naringenin streptozotocin oxidative stress inflammation Specialties of internal medicine RC581-951 Zhao C Yin X Zhao C The Renoprotective Effects of Naringenin (NGN) in Gestational Pregnancy
description	Chunrong Zhao,1 Xiufeng Yin,2 Chunping Zhao1 1Department of Obstetrics, Linyi Central Hospital of Shandong Province, Linyi, Shandong 276400, People’s Republic of China; 2Department of Obstetrics and Gynecology, Yixing People’s Hospital, Wuxi, Jiangsu 214200, People’s Republic of ChinaCorrespondence: Xiufeng YinDepartment of Obstetrics and Gynecology, Yixing People’s Hospital, No. 75 Tongzhen Guan Road, Yicheng, Yixing, Jiangsu 214200, People’s Republic of ChinaTel +86-510-8733 0741Email yin393439460@163.comIntroduction: Gestational diabetes mellitus (GDM) is defined as glucose intolerance that is first diagnosed during pregnancy, a condition risking the health of both the mother and the baby. Naringenin (NGN) has been demonstrated to have multiple therapeutic functions, while it is also considered to exhibit antidiabetic properties. The present study aimed to investigate the protective effects of NGN in pregnant diabetic rats.Methods: GDM was induced by feeding the rats with a high-fat diet for 5 weeks, followed by intraperitoneal injection of streptozotocin (35 mg/kg). The fasting blood glucose were determined with a glucometer and the 24-h urine protein (24-UPro) were determined by the sulfonyl salicylic acid method. The pathological morphological changes and apoptosis of glomeruli cells of kidney tissue using hematoxylin and eosin (H&E) staining and TUNEL analysis. Enzyme-linked immunosorbent assay (ELISA) kits were used to detect the serum T-AOC, the activity of SOD, the levels of GSH-Px, CAT and MDA, TNF-α, IL-6, TGF-β, ICAM-1.The expression of related genes were measured by RT-qPCR and Western blot analyses.Results: In the NGN-treated group, it was observed that the general status of the rats was improved, while the levels of blood glucose and 24-UPro were significantly decreased. In addition, the histopathological changes in renal tissues and renal cell apoptosis were significantly improved upon treatment with NGN. The expression levels of oxidative stress and inflammation-associated factors also differed signifigcantly between the model and NGN-treated groups. Upon treatment with NGN, the levels of peroxisome proliferator-activated receptor α were significantly increased, while the activity of enzymes involved in the oxidative metabolism of fatty acids was significantly decreased.Conclusion: These preliminary experimental findings demonstrate that NGN has a certain renoprotective effect on GDM, which provides a novel therapeutic option for this condition.Keywords: gestational diabetes mellitus, Naringenin, streptozotocin, oxidative stress, inflammation
format	article
author	Zhao C Yin X Zhao C
author_facet	Zhao C Yin X Zhao C
author_sort	Zhao C
title	The Renoprotective Effects of Naringenin (NGN) in Gestational Pregnancy
title_short	The Renoprotective Effects of Naringenin (NGN) in Gestational Pregnancy
title_full	The Renoprotective Effects of Naringenin (NGN) in Gestational Pregnancy
title_fullStr	The Renoprotective Effects of Naringenin (NGN) in Gestational Pregnancy
title_full_unstemmed	The Renoprotective Effects of Naringenin (NGN) in Gestational Pregnancy
title_sort	renoprotective effects of naringenin (ngn) in gestational pregnancy
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/f1786c955658488ea0d097eed7ec9045
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The Renoprotective Effects of Naringenin (NGN) in Gestational Pregnancy

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