Diminished Expression of Galectin-3 Around Blisters in Bullous Pemphigoid: An Immunohistochemistry Study

Background: Bullous pemphigoid (BP) is a subepidermal blistering disorder caused by autoantibodies directed against hemidesmosomal proteins. Many patients with BP demonstrate circulating IgE autoantibodies. Although the role of IgE in the pathogenesis of BP is unknown, a correlation between IgE ant...

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Autores principales:	Maryam Aghighi, Bruce Smoller
Formato:	article
Lenguaje:	EN
Publicado:	Mattioli1885 2020
Materias:	bullous pemphigoid galectin-3 blister formation Dermatology RL1-803
Acceso en línea:	https://doaj.org/article/f18016d713f74b3f87f34d4648ba47ed
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spelling	oai:doaj.org-article:f18016d713f74b3f87f34d4648ba47ed2021-11-17T08:28:26ZDiminished Expression of Galectin-3 Around Blisters in Bullous Pemphigoid: An Immunohistochemistry Study10.5826/dpc.1004a1062160-9381https://doaj.org/article/f18016d713f74b3f87f34d4648ba47ed2020-10-01T00:00:00Zhttp://dpcj.org/index.php/dpc/article/view/1340https://doaj.org/toc/2160-9381 Background: Bullous pemphigoid (BP) is a subepidermal blistering disorder caused by autoantibodies directed against hemidesmosomal proteins. Many patients with BP demonstrate circulating IgE autoantibodies. Although the role of IgE in the pathogenesis of BP is unknown, a correlation between IgE antibodies and eosinophilia has been observed. Soluble CD23 and galectin-3 are the main elements of the IgE group. The roles for CD23 in BP as a potential biomarker and IgE production regulator have been characterized, but no studies have evaluated any roles for galectin-3 in this disease. Objectives: In this study, we evaluated galectin-3 expression in BP as a first step in assessing its role in the pathogenesis of this autoimmune blistering process. Patients and Methods: Sixty specimens diagnosed as BP were stained with antibodies to galectin-3. The percentages of nuclear and cytoplasmic galectin-3 expression and staining intensity were evaluated. Results: There was a significant difference in galectin-3 cytoplasmic and nuclear expression within keratinocytes immediately surrounding and above the blisters: (1) cytoplasmic (mean = 17.2% ± 2.4%) vs adjacent unaffected skin (mean = 66.7% ± 2.0%, P < 0.0001) and (2) nuclear (mean = 1.9% ± 0.4%) vs adjacent unaffected skin (mean = 13.2% ± 1.2%, P < 0.0001). Conclusions: Lower expression of galectin-3 around blisters in BP may suggest a role as an adhesion molecule. Loss of galectin-3 may add to the extension of blister formation by initiating cell-extracellular matrix disassembly and may be involved with the associated dermal inflammation and the eosinophil chemotaxis. Further studies will be necessary to elucidate the result of this observed loss on disease pathogenesis. Maryam AghighiBruce SmollerMattioli1885articlebullous pemphigoidgalectin-3blister formationDermatologyRL1-803ENDermatology Practical & Conceptual, Vol 10, Iss 4 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	bullous pemphigoid galectin-3 blister formation Dermatology RL1-803
spellingShingle	bullous pemphigoid galectin-3 blister formation Dermatology RL1-803 Maryam Aghighi Bruce Smoller Diminished Expression of Galectin-3 Around Blisters in Bullous Pemphigoid: An Immunohistochemistry Study
description	Background: Bullous pemphigoid (BP) is a subepidermal blistering disorder caused by autoantibodies directed against hemidesmosomal proteins. Many patients with BP demonstrate circulating IgE autoantibodies. Although the role of IgE in the pathogenesis of BP is unknown, a correlation between IgE antibodies and eosinophilia has been observed. Soluble CD23 and galectin-3 are the main elements of the IgE group. The roles for CD23 in BP as a potential biomarker and IgE production regulator have been characterized, but no studies have evaluated any roles for galectin-3 in this disease. Objectives: In this study, we evaluated galectin-3 expression in BP as a first step in assessing its role in the pathogenesis of this autoimmune blistering process. Patients and Methods: Sixty specimens diagnosed as BP were stained with antibodies to galectin-3. The percentages of nuclear and cytoplasmic galectin-3 expression and staining intensity were evaluated. Results: There was a significant difference in galectin-3 cytoplasmic and nuclear expression within keratinocytes immediately surrounding and above the blisters: (1) cytoplasmic (mean = 17.2% ± 2.4%) vs adjacent unaffected skin (mean = 66.7% ± 2.0%, P < 0.0001) and (2) nuclear (mean = 1.9% ± 0.4%) vs adjacent unaffected skin (mean = 13.2% ± 1.2%, P < 0.0001). Conclusions: Lower expression of galectin-3 around blisters in BP may suggest a role as an adhesion molecule. Loss of galectin-3 may add to the extension of blister formation by initiating cell-extracellular matrix disassembly and may be involved with the associated dermal inflammation and the eosinophil chemotaxis. Further studies will be necessary to elucidate the result of this observed loss on disease pathogenesis.
format	article
author	Maryam Aghighi Bruce Smoller
author_facet	Maryam Aghighi Bruce Smoller
author_sort	Maryam Aghighi
title	Diminished Expression of Galectin-3 Around Blisters in Bullous Pemphigoid: An Immunohistochemistry Study
title_short	Diminished Expression of Galectin-3 Around Blisters in Bullous Pemphigoid: An Immunohistochemistry Study
title_full	Diminished Expression of Galectin-3 Around Blisters in Bullous Pemphigoid: An Immunohistochemistry Study
title_fullStr	Diminished Expression of Galectin-3 Around Blisters in Bullous Pemphigoid: An Immunohistochemistry Study
title_full_unstemmed	Diminished Expression of Galectin-3 Around Blisters in Bullous Pemphigoid: An Immunohistochemistry Study
title_sort	diminished expression of galectin-3 around blisters in bullous pemphigoid: an immunohistochemistry study
publisher	Mattioli1885
publishDate	2020
url	https://doaj.org/article/f18016d713f74b3f87f34d4648ba47ed
work_keys_str_mv	AT maryamaghighi diminishedexpressionofgalectin3aroundblistersinbullouspemphigoidanimmunohistochemistrystudy AT brucesmoller diminishedexpressionofgalectin3aroundblistersinbullouspemphigoidanimmunohistochemistrystudy
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Diminished Expression of Galectin-3 Around Blisters in Bullous Pemphigoid: An Immunohistochemistry Study

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