L. rhamnosus improves the immune response and tryptophan catabolism in laying hen pullets

Abstract In mammals, early-life probiotic supplementation is a promising tool for preventing unfavourable, gut microbiome-related behavioural, immunological, and aromatic amino acid alterations later in life. In laying hens, feather-pecking behaviour is proposed to be a consequence of gut-brain axis...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Claire Mindus, Nienke van Staaveren, Dietmar Fuchs, Johanna M. Gostner, Joergen B. Kjaer, Wolfgang Kunze, M. Firoz Mian, Anna K. Shoveller, Paul Forsythe, Alexandra Harlander-Matauschek
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f182391a037a43ce94bdf3115bf5e692
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f182391a037a43ce94bdf3115bf5e692
record_format	dspace
spelling	oai:doaj.org-article:f182391a037a43ce94bdf3115bf5e6922021-12-02T18:51:35ZL. rhamnosus improves the immune response and tryptophan catabolism in laying hen pullets10.1038/s41598-021-98459-x2045-2322https://doaj.org/article/f182391a037a43ce94bdf3115bf5e6922021-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98459-xhttps://doaj.org/toc/2045-2322Abstract In mammals, early-life probiotic supplementation is a promising tool for preventing unfavourable, gut microbiome-related behavioural, immunological, and aromatic amino acid alterations later in life. In laying hens, feather-pecking behaviour is proposed to be a consequence of gut-brain axis dysregulation. Lactobacillus rhamnosus decreases stress-induced severe feather pecking in adult hens, but whether its effect in pullets is more robust is unknown. Consequently, we investigated whether early-life, oral supplementation with a single Lactobacillus rhamnosus strain can prevent stress-induced feather-pecking behaviour in chickens. To this end, we monitored both the short- and long-term effects of the probiotic supplement on behaviour and related physiological parameters. We hypothesized that L. rhamnosus would reduce pecking behaviour by modulating the biological pathways associated with this detrimental behaviour, namely aromatic amino acid turnover linked to neurotransmitter production and stress-related immune responses. We report that stress decreased the proportion of cytotoxic T cells in the tonsils (P = 0.047). Counteracting this T cell depression, birds receiving the L. rhamnosus supplementation significantly increased all T lymphocyte subset proportions (P < 0.05). Both phenotypic and genotypic feather peckers had lower plasma tryptophan concentrations compared to their non-pecking counterparts. The probiotic supplement caused a short-term increase in plasma tryptophan (P < 0.001) and the TRP:(PHE + TYR) ratio (P < 0.001). The administration of stressors did not significantly increase feather pecking in pullets, an observation consistent with the age-dependent onset of pecking behaviour. Despite minimal changes to behaviour, our data demonstrate the impact of L. rhamnosus supplementation on the immune system and the turnover of the serotonin precursor tryptophan. Our findings indicate that L. rhamnosus exerts a transient, beneficial effect on the immune response and tryptophan catabolism in pullets.Claire MindusNienke van StaaverenDietmar FuchsJohanna M. GostnerJoergen B. KjaerWolfgang KunzeM. Firoz MianAnna K. ShovellerPaul ForsytheAlexandra Harlander-MatauschekNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Claire Mindus Nienke van Staaveren Dietmar Fuchs Johanna M. Gostner Joergen B. Kjaer Wolfgang Kunze M. Firoz Mian Anna K. Shoveller Paul Forsythe Alexandra Harlander-Matauschek L. rhamnosus improves the immune response and tryptophan catabolism in laying hen pullets
description	Abstract In mammals, early-life probiotic supplementation is a promising tool for preventing unfavourable, gut microbiome-related behavioural, immunological, and aromatic amino acid alterations later in life. In laying hens, feather-pecking behaviour is proposed to be a consequence of gut-brain axis dysregulation. Lactobacillus rhamnosus decreases stress-induced severe feather pecking in adult hens, but whether its effect in pullets is more robust is unknown. Consequently, we investigated whether early-life, oral supplementation with a single Lactobacillus rhamnosus strain can prevent stress-induced feather-pecking behaviour in chickens. To this end, we monitored both the short- and long-term effects of the probiotic supplement on behaviour and related physiological parameters. We hypothesized that L. rhamnosus would reduce pecking behaviour by modulating the biological pathways associated with this detrimental behaviour, namely aromatic amino acid turnover linked to neurotransmitter production and stress-related immune responses. We report that stress decreased the proportion of cytotoxic T cells in the tonsils (P = 0.047). Counteracting this T cell depression, birds receiving the L. rhamnosus supplementation significantly increased all T lymphocyte subset proportions (P < 0.05). Both phenotypic and genotypic feather peckers had lower plasma tryptophan concentrations compared to their non-pecking counterparts. The probiotic supplement caused a short-term increase in plasma tryptophan (P < 0.001) and the TRP:(PHE + TYR) ratio (P < 0.001). The administration of stressors did not significantly increase feather pecking in pullets, an observation consistent with the age-dependent onset of pecking behaviour. Despite minimal changes to behaviour, our data demonstrate the impact of L. rhamnosus supplementation on the immune system and the turnover of the serotonin precursor tryptophan. Our findings indicate that L. rhamnosus exerts a transient, beneficial effect on the immune response and tryptophan catabolism in pullets.
format	article
author	Claire Mindus Nienke van Staaveren Dietmar Fuchs Johanna M. Gostner Joergen B. Kjaer Wolfgang Kunze M. Firoz Mian Anna K. Shoveller Paul Forsythe Alexandra Harlander-Matauschek
author_facet	Claire Mindus Nienke van Staaveren Dietmar Fuchs Johanna M. Gostner Joergen B. Kjaer Wolfgang Kunze M. Firoz Mian Anna K. Shoveller Paul Forsythe Alexandra Harlander-Matauschek
author_sort	Claire Mindus
title	L. rhamnosus improves the immune response and tryptophan catabolism in laying hen pullets
title_short	L. rhamnosus improves the immune response and tryptophan catabolism in laying hen pullets
title_full	L. rhamnosus improves the immune response and tryptophan catabolism in laying hen pullets
title_fullStr	L. rhamnosus improves the immune response and tryptophan catabolism in laying hen pullets
title_full_unstemmed	L. rhamnosus improves the immune response and tryptophan catabolism in laying hen pullets
title_sort	l. rhamnosus improves the immune response and tryptophan catabolism in laying hen pullets
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f182391a037a43ce94bdf3115bf5e692
work_keys_str_mv	AT clairemindus lrhamnosusimprovestheimmuneresponseandtryptophancatabolisminlayinghenpullets AT nienkevanstaaveren lrhamnosusimprovestheimmuneresponseandtryptophancatabolisminlayinghenpullets AT dietmarfuchs lrhamnosusimprovestheimmuneresponseandtryptophancatabolisminlayinghenpullets AT johannamgostner lrhamnosusimprovestheimmuneresponseandtryptophancatabolisminlayinghenpullets AT joergenbkjaer lrhamnosusimprovestheimmuneresponseandtryptophancatabolisminlayinghenpullets AT wolfgangkunze lrhamnosusimprovestheimmuneresponseandtryptophancatabolisminlayinghenpullets AT mfirozmian lrhamnosusimprovestheimmuneresponseandtryptophancatabolisminlayinghenpullets AT annakshoveller lrhamnosusimprovestheimmuneresponseandtryptophancatabolisminlayinghenpullets AT paulforsythe lrhamnosusimprovestheimmuneresponseandtryptophancatabolisminlayinghenpullets AT alexandraharlandermatauschek lrhamnosusimprovestheimmuneresponseandtryptophancatabolisminlayinghenpullets
_version_	1718377421432946688

L. rhamnosus improves the immune response and tryptophan catabolism in laying hen pullets

Ejemplares similares