Marker-assisted pyramiding of lycopene-ε-cyclase, β-carotene hydroxylase1 and opaque2 genes for development of biofortified maize hybrids

Abstract Malnutrition affects growth and development in humans and causes socio-economic losses. Normal maize is deficient in essential amino acids, lysine and tryptophan; and vitamin-A. Crop biofortification is a sustainable and economical approach to alleviate micronutrient malnutrition. We combin...

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Autores principales:	Jagveer Singh, Shikha Sharma, Amandeep Kaur, Yogesh Vikal, Amandeep Kaur Cheema, Balraj Kaur Bains, Noorpreet Kaur, Gurjit Kaur Gill, Pawan Kumar Malhotra, Ashok Kumar, Priti Sharma, Vignesh Muthusamy, Amarjeet Kaur, Jasbir Singh Chawla, Firoz Hossain
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f18515b4dad8434db0b74552f3086126
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spelling	oai:doaj.org-article:f18515b4dad8434db0b74552f30861262021-12-02T17:41:04ZMarker-assisted pyramiding of lycopene-ε-cyclase, β-carotene hydroxylase1 and opaque2 genes for development of biofortified maize hybrids10.1038/s41598-021-92010-82045-2322https://doaj.org/article/f18515b4dad8434db0b74552f30861262021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92010-8https://doaj.org/toc/2045-2322Abstract Malnutrition affects growth and development in humans and causes socio-economic losses. Normal maize is deficient in essential amino acids, lysine and tryptophan; and vitamin-A. Crop biofortification is a sustainable and economical approach to alleviate micronutrient malnutrition. We combined favorable alleles of crtRB1 and lcyE genes into opaque2 (o2)-based four inbreds viz. QLM11, QLM12, QLM13, and QLM14 using marker-assisted backcross breeding. These are parents of quality protein maize versions of two elite hybrids viz. Buland and PMH1, grown in India. Gene-based SSRs for o2 and InDel markers for crtRB1 and lcyE were successfully employed for foreground selection in BC1F1, BC2F1, and BC2F2 generations. The recurrent parent genome recovery ranged from 88.9 to 96.0% among introgressed progenies. Kernels of pyramided lines possessed a high concentration of proA (7.14–9.63 ppm), compared to 1.05 to 1.41 ppm in the recurrent parents, while lysine and tryptophan ranged from 0.28–0.44% and 0.07–0.09%, respectively. The reconstituted hybrids (RBuland and RPMH1) showed significant enhancement of endosperm proA (6.97–9.82 ppm), tryptophan (0.07–0.09%), and lysine (0.29–0.43%), while grain yield was at par with their original versions. The dissemination of reconstituted hybrids holds significant promise to alleviate vitamin-A deficiency and protein-energy malnutrition in developing countries.Jagveer SinghShikha SharmaAmandeep KaurYogesh VikalAmandeep Kaur CheemaBalraj Kaur BainsNoorpreet KaurGurjit Kaur GillPawan Kumar MalhotraAshok KumarPriti SharmaVignesh MuthusamyAmarjeet KaurJasbir Singh ChawlaFiroz HossainNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jagveer Singh Shikha Sharma Amandeep Kaur Yogesh Vikal Amandeep Kaur Cheema Balraj Kaur Bains Noorpreet Kaur Gurjit Kaur Gill Pawan Kumar Malhotra Ashok Kumar Priti Sharma Vignesh Muthusamy Amarjeet Kaur Jasbir Singh Chawla Firoz Hossain Marker-assisted pyramiding of lycopene-ε-cyclase, β-carotene hydroxylase1 and opaque2 genes for development of biofortified maize hybrids
description	Abstract Malnutrition affects growth and development in humans and causes socio-economic losses. Normal maize is deficient in essential amino acids, lysine and tryptophan; and vitamin-A. Crop biofortification is a sustainable and economical approach to alleviate micronutrient malnutrition. We combined favorable alleles of crtRB1 and lcyE genes into opaque2 (o2)-based four inbreds viz. QLM11, QLM12, QLM13, and QLM14 using marker-assisted backcross breeding. These are parents of quality protein maize versions of two elite hybrids viz. Buland and PMH1, grown in India. Gene-based SSRs for o2 and InDel markers for crtRB1 and lcyE were successfully employed for foreground selection in BC1F1, BC2F1, and BC2F2 generations. The recurrent parent genome recovery ranged from 88.9 to 96.0% among introgressed progenies. Kernels of pyramided lines possessed a high concentration of proA (7.14–9.63 ppm), compared to 1.05 to 1.41 ppm in the recurrent parents, while lysine and tryptophan ranged from 0.28–0.44% and 0.07–0.09%, respectively. The reconstituted hybrids (RBuland and RPMH1) showed significant enhancement of endosperm proA (6.97–9.82 ppm), tryptophan (0.07–0.09%), and lysine (0.29–0.43%), while grain yield was at par with their original versions. The dissemination of reconstituted hybrids holds significant promise to alleviate vitamin-A deficiency and protein-energy malnutrition in developing countries.
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author	Jagveer Singh Shikha Sharma Amandeep Kaur Yogesh Vikal Amandeep Kaur Cheema Balraj Kaur Bains Noorpreet Kaur Gurjit Kaur Gill Pawan Kumar Malhotra Ashok Kumar Priti Sharma Vignesh Muthusamy Amarjeet Kaur Jasbir Singh Chawla Firoz Hossain
author_facet	Jagveer Singh Shikha Sharma Amandeep Kaur Yogesh Vikal Amandeep Kaur Cheema Balraj Kaur Bains Noorpreet Kaur Gurjit Kaur Gill Pawan Kumar Malhotra Ashok Kumar Priti Sharma Vignesh Muthusamy Amarjeet Kaur Jasbir Singh Chawla Firoz Hossain
author_sort	Jagveer Singh
title	Marker-assisted pyramiding of lycopene-ε-cyclase, β-carotene hydroxylase1 and opaque2 genes for development of biofortified maize hybrids
title_short	Marker-assisted pyramiding of lycopene-ε-cyclase, β-carotene hydroxylase1 and opaque2 genes for development of biofortified maize hybrids
title_full	Marker-assisted pyramiding of lycopene-ε-cyclase, β-carotene hydroxylase1 and opaque2 genes for development of biofortified maize hybrids
title_fullStr	Marker-assisted pyramiding of lycopene-ε-cyclase, β-carotene hydroxylase1 and opaque2 genes for development of biofortified maize hybrids
title_full_unstemmed	Marker-assisted pyramiding of lycopene-ε-cyclase, β-carotene hydroxylase1 and opaque2 genes for development of biofortified maize hybrids
title_sort	marker-assisted pyramiding of lycopene-ε-cyclase, β-carotene hydroxylase1 and opaque2 genes for development of biofortified maize hybrids
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f18515b4dad8434db0b74552f3086126
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Marker-assisted pyramiding of lycopene-ε-cyclase, β-carotene hydroxylase1 and opaque2 genes for development of biofortified maize hybrids

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