Phosphatidic acid increases Notch signalling by affecting Sanpodo trafficking during Drosophila sensory organ development

Abstract Organ cell diversity depends on binary cell-fate decisions mediated by the Notch signalling pathway during development and tissue homeostasis. A clear example is the series of binary cell-fate decisions that take place during asymmetric cell divisions that give rise to the sensory organs of...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ignacio Medina-Yáñez, Gonzalo H. Olivares, Franco Vega-Macaya, Marek Mlodzik, Patricio Olguín
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f18663aa686d425ca097bcd249292927
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f18663aa686d425ca097bcd249292927
record_format	dspace
spelling	oai:doaj.org-article:f18663aa686d425ca097bcd2492929272021-12-02T12:33:04ZPhosphatidic acid increases Notch signalling by affecting Sanpodo trafficking during Drosophila sensory organ development10.1038/s41598-020-78831-z2045-2322https://doaj.org/article/f18663aa686d425ca097bcd2492929272020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78831-zhttps://doaj.org/toc/2045-2322Abstract Organ cell diversity depends on binary cell-fate decisions mediated by the Notch signalling pathway during development and tissue homeostasis. A clear example is the series of binary cell-fate decisions that take place during asymmetric cell divisions that give rise to the sensory organs of Drosophila melanogaster. The regulated trafficking of Sanpodo, a transmembrane protein that potentiates receptor activity, plays a pivotal role in this process. Membrane lipids can regulate many signalling pathways by affecting receptor and ligand trafficking. It remains unknown, however, whether phosphatidic acid regulates Notch-mediated binary cell-fate decisions during asymmetric cell divisions, and what are the cellular mechanisms involved. Here we show that increased phosphatidic acid derived from Phospholipase D leads to defects in binary cell-fate decisions that are compatible with ectopic Notch activation in precursor cells, where it is normally inactive. Null mutants of numb or the α-subunit of Adaptor Protein complex-2 enhance dominantly this phenotype while removing a copy of Notch or sanpodo suppresses it. In vivo analyses show that Sanpodo localization decreases at acidic compartments, associated with increased internalization of Notch. We propose that Phospholipase D-derived phosphatidic acid promotes ectopic Notch signalling by increasing receptor endocytosis and inhibiting Sanpodo trafficking towards acidic endosomes.Ignacio Medina-YáñezGonzalo H. OlivaresFranco Vega-MacayaMarek MlodzikPatricio OlguínNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-12 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ignacio Medina-Yáñez Gonzalo H. Olivares Franco Vega-Macaya Marek Mlodzik Patricio Olguín Phosphatidic acid increases Notch signalling by affecting Sanpodo trafficking during Drosophila sensory organ development
description	Abstract Organ cell diversity depends on binary cell-fate decisions mediated by the Notch signalling pathway during development and tissue homeostasis. A clear example is the series of binary cell-fate decisions that take place during asymmetric cell divisions that give rise to the sensory organs of Drosophila melanogaster. The regulated trafficking of Sanpodo, a transmembrane protein that potentiates receptor activity, plays a pivotal role in this process. Membrane lipids can regulate many signalling pathways by affecting receptor and ligand trafficking. It remains unknown, however, whether phosphatidic acid regulates Notch-mediated binary cell-fate decisions during asymmetric cell divisions, and what are the cellular mechanisms involved. Here we show that increased phosphatidic acid derived from Phospholipase D leads to defects in binary cell-fate decisions that are compatible with ectopic Notch activation in precursor cells, where it is normally inactive. Null mutants of numb or the α-subunit of Adaptor Protein complex-2 enhance dominantly this phenotype while removing a copy of Notch or sanpodo suppresses it. In vivo analyses show that Sanpodo localization decreases at acidic compartments, associated with increased internalization of Notch. We propose that Phospholipase D-derived phosphatidic acid promotes ectopic Notch signalling by increasing receptor endocytosis and inhibiting Sanpodo trafficking towards acidic endosomes.
format	article
author	Ignacio Medina-Yáñez Gonzalo H. Olivares Franco Vega-Macaya Marek Mlodzik Patricio Olguín
author_facet	Ignacio Medina-Yáñez Gonzalo H. Olivares Franco Vega-Macaya Marek Mlodzik Patricio Olguín
author_sort	Ignacio Medina-Yáñez
title	Phosphatidic acid increases Notch signalling by affecting Sanpodo trafficking during Drosophila sensory organ development
title_short	Phosphatidic acid increases Notch signalling by affecting Sanpodo trafficking during Drosophila sensory organ development
title_full	Phosphatidic acid increases Notch signalling by affecting Sanpodo trafficking during Drosophila sensory organ development
title_fullStr	Phosphatidic acid increases Notch signalling by affecting Sanpodo trafficking during Drosophila sensory organ development
title_full_unstemmed	Phosphatidic acid increases Notch signalling by affecting Sanpodo trafficking during Drosophila sensory organ development
title_sort	phosphatidic acid increases notch signalling by affecting sanpodo trafficking during drosophila sensory organ development
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/f18663aa686d425ca097bcd249292927
work_keys_str_mv	AT ignaciomedinayanez phosphatidicacidincreasesnotchsignallingbyaffectingsanpodotraffickingduringdrosophilasensoryorgandevelopment AT gonzaloholivares phosphatidicacidincreasesnotchsignallingbyaffectingsanpodotraffickingduringdrosophilasensoryorgandevelopment AT francovegamacaya phosphatidicacidincreasesnotchsignallingbyaffectingsanpodotraffickingduringdrosophilasensoryorgandevelopment AT marekmlodzik phosphatidicacidincreasesnotchsignallingbyaffectingsanpodotraffickingduringdrosophilasensoryorgandevelopment AT patricioolguin phosphatidicacidincreasesnotchsignallingbyaffectingsanpodotraffickingduringdrosophilasensoryorgandevelopment
_version_	1718393894522060800

Phosphatidic acid increases Notch signalling by affecting Sanpodo trafficking during Drosophila sensory organ development

Ejemplares similares