Comprehensive Transcriptome and Metabolic Profiling of Petal Color Development in Lycoris sprengeri

Lycoris sprengeri (L. sprengeri) is an important ornamental bulbous plant, and its numerous varieties in different color forms are widely planted. Multiple color types of petals in L. sprengeri provide us with possibilities to delineate the complicated metabolic networks underlying the biochemical t...

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Autores principales:	Feng Yang, Chao-han Li, Debatosh Das, Yu-hong Zheng, Tao Song, Lan-xiang Wang, Mo-Xian Chen, Qing-zhu Li, Jianhua Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Lycoris sprengeri flavonoids transcriptome anthocyanin petal color metabolome Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/f1875049549e4eaea33d65f4fb5c725e
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spelling	oai:doaj.org-article:f1875049549e4eaea33d65f4fb5c725e2021-12-03T07:18:07ZComprehensive Transcriptome and Metabolic Profiling of Petal Color Development in Lycoris sprengeri1664-462X10.3389/fpls.2021.747131https://doaj.org/article/f1875049549e4eaea33d65f4fb5c725e2021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.747131/fullhttps://doaj.org/toc/1664-462XLycoris sprengeri (L. sprengeri) is an important ornamental bulbous plant, and its numerous varieties in different color forms are widely planted. Multiple color types of petals in L. sprengeri provide us with possibilities to delineate the complicated metabolic networks underlying the biochemical traits behind color formation in this plant species, especially petal color. In this study, we sequenced and annotated a reference transcriptome of pink and white petals of L. sprengeri and analyzed the metabolic role of anthocyanin biosynthesis in regulating color pigment metabolism. Briefly, white and pink petal samples were sequenced with an Illumina platform, to obtain the reads that could be assembled into 100,778 unique sequences. Sequences expressed differentially between white vs. pink petals were further annotated with the terms of Gene Ontology (GO), Clusters of Orthologous Groups (COG), Kyoto Encyclopedia of Genes and Genomes (KEGG), and eggNOG. Gene expression analyses revealed the repression of anthocyanin and steroid biosynthesis enzymes and R2R3 MYB transcription factor (TF) genes in white petals compared to pink petals. Furthermore, the targeted metabolic profiling of anthocyanins revealed that color-related delphinidin (Del) and cyanidin (Cy) pigments are lower in white petals, which correlate well with the reduced gene expression levels of anthocyanin biosynthesis genes. Taken together, it is hypothesized that anthocyanin biosynthesis, steroid biosynthesis, and R2R3 MYB TFs may play vital regulatory roles in petal color development in L. sprengeri. This work provides a valuable genomic resource for flower breeding and metabolic engineering in horticulture and markers for studying the flower trait evolution of L. sprengeri.Feng YangFeng YangChao-han LiDebatosh DasYu-hong ZhengTao SongLan-xiang WangMo-Xian ChenQing-zhu LiJianhua ZhangFrontiers Media S.A.articleLycoris sprengeriflavonoidstranscriptomeanthocyaninpetal colormetabolomePlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Lycoris sprengeri flavonoids transcriptome anthocyanin petal color metabolome Plant culture SB1-1110
spellingShingle	Lycoris sprengeri flavonoids transcriptome anthocyanin petal color metabolome Plant culture SB1-1110 Feng Yang Feng Yang Chao-han Li Debatosh Das Yu-hong Zheng Tao Song Lan-xiang Wang Mo-Xian Chen Qing-zhu Li Jianhua Zhang Comprehensive Transcriptome and Metabolic Profiling of Petal Color Development in Lycoris sprengeri
description	Lycoris sprengeri (L. sprengeri) is an important ornamental bulbous plant, and its numerous varieties in different color forms are widely planted. Multiple color types of petals in L. sprengeri provide us with possibilities to delineate the complicated metabolic networks underlying the biochemical traits behind color formation in this plant species, especially petal color. In this study, we sequenced and annotated a reference transcriptome of pink and white petals of L. sprengeri and analyzed the metabolic role of anthocyanin biosynthesis in regulating color pigment metabolism. Briefly, white and pink petal samples were sequenced with an Illumina platform, to obtain the reads that could be assembled into 100,778 unique sequences. Sequences expressed differentially between white vs. pink petals were further annotated with the terms of Gene Ontology (GO), Clusters of Orthologous Groups (COG), Kyoto Encyclopedia of Genes and Genomes (KEGG), and eggNOG. Gene expression analyses revealed the repression of anthocyanin and steroid biosynthesis enzymes and R2R3 MYB transcription factor (TF) genes in white petals compared to pink petals. Furthermore, the targeted metabolic profiling of anthocyanins revealed that color-related delphinidin (Del) and cyanidin (Cy) pigments are lower in white petals, which correlate well with the reduced gene expression levels of anthocyanin biosynthesis genes. Taken together, it is hypothesized that anthocyanin biosynthesis, steroid biosynthesis, and R2R3 MYB TFs may play vital regulatory roles in petal color development in L. sprengeri. This work provides a valuable genomic resource for flower breeding and metabolic engineering in horticulture and markers for studying the flower trait evolution of L. sprengeri.
format	article
author	Feng Yang Feng Yang Chao-han Li Debatosh Das Yu-hong Zheng Tao Song Lan-xiang Wang Mo-Xian Chen Qing-zhu Li Jianhua Zhang
author_facet	Feng Yang Feng Yang Chao-han Li Debatosh Das Yu-hong Zheng Tao Song Lan-xiang Wang Mo-Xian Chen Qing-zhu Li Jianhua Zhang
author_sort	Feng Yang
title	Comprehensive Transcriptome and Metabolic Profiling of Petal Color Development in Lycoris sprengeri
title_short	Comprehensive Transcriptome and Metabolic Profiling of Petal Color Development in Lycoris sprengeri
title_full	Comprehensive Transcriptome and Metabolic Profiling of Petal Color Development in Lycoris sprengeri
title_fullStr	Comprehensive Transcriptome and Metabolic Profiling of Petal Color Development in Lycoris sprengeri
title_full_unstemmed	Comprehensive Transcriptome and Metabolic Profiling of Petal Color Development in Lycoris sprengeri
title_sort	comprehensive transcriptome and metabolic profiling of petal color development in lycoris sprengeri
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/f1875049549e4eaea33d65f4fb5c725e
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Comprehensive Transcriptome and Metabolic Profiling of Petal Color Development in Lycoris sprengeri

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