Endogenous melatonin promotes rhythmic recruitment of neutrophils toward an injury in zebrafish

Abstract Neutrophil recruitment to injured tissue appears to be an evolutionarily conserved strategy for organisms to fight against exogenous insults. Recent studies have shown rhythmic migration of neutrophils and several factors, including melatonin, have been implicated in regulating this rhythmi...

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Autores principales:	Da-long Ren, Cheng Ji, Xiao-Bo Wang, Han Wang, Bing Hu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f18889151ab64d6c8279edb0412b2cf7
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spelling	oai:doaj.org-article:f18889151ab64d6c8279edb0412b2cf72021-12-02T11:52:55ZEndogenous melatonin promotes rhythmic recruitment of neutrophils toward an injury in zebrafish10.1038/s41598-017-05074-w2045-2322https://doaj.org/article/f18889151ab64d6c8279edb0412b2cf72017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05074-whttps://doaj.org/toc/2045-2322Abstract Neutrophil recruitment to injured tissue appears to be an evolutionarily conserved strategy for organisms to fight against exogenous insults. Recent studies have shown rhythmic migration of neutrophils and several factors, including melatonin, have been implicated in regulating this rhythmic migration. The mechanisms underlying how endogenous melatonin regulates rhythmic neutrophils migration, however, are unclear. Here we generated a zebrafish annat2 mutant that lacks endogenous melatonin and, subsequently, a Tg(lyz:EGFP);aanat2 −/− transgenic line that allows for monitoring neutrophils migration visually in live zebrafish. We observed that migrating neutrophils are significantly reduced in aanat2 −/− mutant zebrafish under a light/dark condition, and the disrupted migrating rhythmicity of neutrophils in aanat2 −/− zebrafish is independent of the circadian clock. Further, we also found that endogenous melatonin enhances neutrophils migration likely by inducing the expression of cytokines such as interleukin-8 and interleukin-1β. Together, our findings provide evidence that endogenous melatonin promotes rhythmic migration of neutrophils through cytokines in zebrafish.Da-long RenCheng JiXiao-Bo WangHan WangBing HuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Da-long Ren Cheng Ji Xiao-Bo Wang Han Wang Bing Hu Endogenous melatonin promotes rhythmic recruitment of neutrophils toward an injury in zebrafish
description	Abstract Neutrophil recruitment to injured tissue appears to be an evolutionarily conserved strategy for organisms to fight against exogenous insults. Recent studies have shown rhythmic migration of neutrophils and several factors, including melatonin, have been implicated in regulating this rhythmic migration. The mechanisms underlying how endogenous melatonin regulates rhythmic neutrophils migration, however, are unclear. Here we generated a zebrafish annat2 mutant that lacks endogenous melatonin and, subsequently, a Tg(lyz:EGFP);aanat2 −/− transgenic line that allows for monitoring neutrophils migration visually in live zebrafish. We observed that migrating neutrophils are significantly reduced in aanat2 −/− mutant zebrafish under a light/dark condition, and the disrupted migrating rhythmicity of neutrophils in aanat2 −/− zebrafish is independent of the circadian clock. Further, we also found that endogenous melatonin enhances neutrophils migration likely by inducing the expression of cytokines such as interleukin-8 and interleukin-1β. Together, our findings provide evidence that endogenous melatonin promotes rhythmic migration of neutrophils through cytokines in zebrafish.
format	article
author	Da-long Ren Cheng Ji Xiao-Bo Wang Han Wang Bing Hu
author_facet	Da-long Ren Cheng Ji Xiao-Bo Wang Han Wang Bing Hu
author_sort	Da-long Ren
title	Endogenous melatonin promotes rhythmic recruitment of neutrophils toward an injury in zebrafish
title_short	Endogenous melatonin promotes rhythmic recruitment of neutrophils toward an injury in zebrafish
title_full	Endogenous melatonin promotes rhythmic recruitment of neutrophils toward an injury in zebrafish
title_fullStr	Endogenous melatonin promotes rhythmic recruitment of neutrophils toward an injury in zebrafish
title_full_unstemmed	Endogenous melatonin promotes rhythmic recruitment of neutrophils toward an injury in zebrafish
title_sort	endogenous melatonin promotes rhythmic recruitment of neutrophils toward an injury in zebrafish
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/f18889151ab64d6c8279edb0412b2cf7
work_keys_str_mv	AT dalongren endogenousmelatoninpromotesrhythmicrecruitmentofneutrophilstowardaninjuryinzebrafish AT chengji endogenousmelatoninpromotesrhythmicrecruitmentofneutrophilstowardaninjuryinzebrafish AT xiaobowang endogenousmelatoninpromotesrhythmicrecruitmentofneutrophilstowardaninjuryinzebrafish AT hanwang endogenousmelatoninpromotesrhythmicrecruitmentofneutrophilstowardaninjuryinzebrafish AT binghu endogenousmelatoninpromotesrhythmicrecruitmentofneutrophilstowardaninjuryinzebrafish
_version_	1718394960588308480

Endogenous melatonin promotes rhythmic recruitment of neutrophils toward an injury in zebrafish

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