A novel dissolution media for testing drug release from a nanostructured polysaccharide-based colon specific drug delivery system: an approach to alternative colon media
Niranjan G Kotla,1,2 Sima Singh,1,3 Balaji Maddiboyina,4 Omprakash Sunnapu,2 Thomas J Webster5,6 1School of Pharmaceutical Sciences, Lovely Professional University, Punjab, India; 2Technologies for the Advancement of Science, Institute for Stem Cell Biology and Regenerative Medicine, Bangalore, Kar...
Guardado en:
| Autores principales: | , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Dove Medical Press
2016
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/f18931020590439988977a29e93aad86 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:f18931020590439988977a29e93aad86 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:f18931020590439988977a29e93aad862021-12-02T06:16:23ZA novel dissolution media for testing drug release from a nanostructured polysaccharide-based colon specific drug delivery system: an approach to alternative colon media1178-2013https://doaj.org/article/f18931020590439988977a29e93aad862016-03-01T00:00:00Zhttps://www.dovepress.com/a-novel-dissolution-media-for-testing-drug-release-from-a-nanostructur-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Niranjan G Kotla,1,2 Sima Singh,1,3 Balaji Maddiboyina,4 Omprakash Sunnapu,2 Thomas J Webster5,6 1School of Pharmaceutical Sciences, Lovely Professional University, Punjab, India; 2Technologies for the Advancement of Science, Institute for Stem Cell Biology and Regenerative Medicine, Bangalore, Karnataka, India; 3Department of Pharmaceutical Sciences, Birla Institute of Technology, Mesra, Ranchi, Jharkhand, India; 4Department of Pharmaceutics, Vishwabharathi College of Pharmaceutical Sciences, Guntur, Andhra Pradesh, India; 5Department of Chemical Engineering, Northeastern University, Boston, MA, USA; 6Center of Excellence for Advanced Materials Research, King Abdulaziz University, Jeddah, Saudi Arabia Abstract: The aim of this study was to develop a novel microbially triggered and animal-sparing dissolution method for testing of nanorough polysaccharide-based micron granules for colonic drug delivery. In this method, probiotic cultures of bacteria present in the colonic region were prepared and added to the dissolution media and compared with the performance of conventional dissolution methodologies (such as media with rat cecal and human fecal media). In this study, the predominant species (such as Bacteroides, Bifidobacterium, Lactobacillus species, Eubacterium and Streptococcus) were cultured in 12% w/v skimmed milk powder and 5% w/v grade “A” honey. Approximately 1010–1011 colony forming units m/L of probiotic culture was added to the dissolution media to test the drug release of polysaccharide-based formulations. A USP dissolution apparatus I/II using a gradient pH dissolution method was used to evaluate drug release from formulations meant for colonic drug delivery. Drug release of guar gum/Eudragit FS30D coated 5-fluorouracil granules was assessed under gastric and small intestine conditions within a simulated colonic environment involving fermentation testing with the probiotic culture. The results with the probiotic system were comparable to those obtained from the rat cecal and human fecal-based fermentation model, thereby suggesting that a probiotic dissolution method can be successfully applied for drug release testing of any polysaccharide-based oral formulation meant for colonic delivery. As such, this study significantly adds to the nanostructured biomaterials’ community by elucidating an easier assay for colonic drug delivery. Keywords: probiotic media, colon specific drug delivery, dissolution methodologies, simulated colonic media, microbially triggered drug deliveryKotla NGSingh SMaddiboyina BSunnapu OWebster TJDove Medical PressarticleProbiotic mediacolon specific drug deliverydissolution methodologiessimulated colonic mediamicrobially triggered drug delivery.Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2016, Iss default, Pp 1089-1095 (2016) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Probiotic media colon specific drug delivery dissolution methodologies simulated colonic media microbially triggered drug delivery. Medicine (General) R5-920 |
| spellingShingle |
Probiotic media colon specific drug delivery dissolution methodologies simulated colonic media microbially triggered drug delivery. Medicine (General) R5-920 Kotla NG Singh S Maddiboyina B Sunnapu O Webster TJ A novel dissolution media for testing drug release from a nanostructured polysaccharide-based colon specific drug delivery system: an approach to alternative colon media |
| description |
Niranjan G Kotla,1,2 Sima Singh,1,3 Balaji Maddiboyina,4 Omprakash Sunnapu,2 Thomas J Webster5,6 1School of Pharmaceutical Sciences, Lovely Professional University, Punjab, India; 2Technologies for the Advancement of Science, Institute for Stem Cell Biology and Regenerative Medicine, Bangalore, Karnataka, India; 3Department of Pharmaceutical Sciences, Birla Institute of Technology, Mesra, Ranchi, Jharkhand, India; 4Department of Pharmaceutics, Vishwabharathi College of Pharmaceutical Sciences, Guntur, Andhra Pradesh, India; 5Department of Chemical Engineering, Northeastern University, Boston, MA, USA; 6Center of Excellence for Advanced Materials Research, King Abdulaziz University, Jeddah, Saudi Arabia Abstract: The aim of this study was to develop a novel microbially triggered and animal-sparing dissolution method for testing of nanorough polysaccharide-based micron granules for colonic drug delivery. In this method, probiotic cultures of bacteria present in the colonic region were prepared and added to the dissolution media and compared with the performance of conventional dissolution methodologies (such as media with rat cecal and human fecal media). In this study, the predominant species (such as Bacteroides, Bifidobacterium, Lactobacillus species, Eubacterium and Streptococcus) were cultured in 12% w/v skimmed milk powder and 5% w/v grade “A” honey. Approximately 1010–1011 colony forming units m/L of probiotic culture was added to the dissolution media to test the drug release of polysaccharide-based formulations. A USP dissolution apparatus I/II using a gradient pH dissolution method was used to evaluate drug release from formulations meant for colonic drug delivery. Drug release of guar gum/Eudragit FS30D coated 5-fluorouracil granules was assessed under gastric and small intestine conditions within a simulated colonic environment involving fermentation testing with the probiotic culture. The results with the probiotic system were comparable to those obtained from the rat cecal and human fecal-based fermentation model, thereby suggesting that a probiotic dissolution method can be successfully applied for drug release testing of any polysaccharide-based oral formulation meant for colonic delivery. As such, this study significantly adds to the nanostructured biomaterials’ community by elucidating an easier assay for colonic drug delivery. Keywords: probiotic media, colon specific drug delivery, dissolution methodologies, simulated colonic media, microbially triggered drug delivery |
| format |
article |
| author |
Kotla NG Singh S Maddiboyina B Sunnapu O Webster TJ |
| author_facet |
Kotla NG Singh S Maddiboyina B Sunnapu O Webster TJ |
| author_sort |
Kotla NG |
| title |
A novel dissolution media for testing drug release from a nanostructured polysaccharide-based colon specific drug delivery system: an approach to alternative colon media |
| title_short |
A novel dissolution media for testing drug release from a nanostructured polysaccharide-based colon specific drug delivery system: an approach to alternative colon media |
| title_full |
A novel dissolution media for testing drug release from a nanostructured polysaccharide-based colon specific drug delivery system: an approach to alternative colon media |
| title_fullStr |
A novel dissolution media for testing drug release from a nanostructured polysaccharide-based colon specific drug delivery system: an approach to alternative colon media |
| title_full_unstemmed |
A novel dissolution media for testing drug release from a nanostructured polysaccharide-based colon specific drug delivery system: an approach to alternative colon media |
| title_sort |
novel dissolution media for testing drug release from a nanostructured polysaccharide-based colon specific drug delivery system: an approach to alternative colon media |
| publisher |
Dove Medical Press |
| publishDate |
2016 |
| url |
https://doaj.org/article/f18931020590439988977a29e93aad86 |
| work_keys_str_mv |
AT kotlang anoveldissolutionmediafortestingdrugreleasefromananostructuredpolysaccharidebasedcolonspecificdrugdeliverysystemanapproachtoalternativecolonmedia AT singhs anoveldissolutionmediafortestingdrugreleasefromananostructuredpolysaccharidebasedcolonspecificdrugdeliverysystemanapproachtoalternativecolonmedia AT maddiboyinab anoveldissolutionmediafortestingdrugreleasefromananostructuredpolysaccharidebasedcolonspecificdrugdeliverysystemanapproachtoalternativecolonmedia AT sunnapuo anoveldissolutionmediafortestingdrugreleasefromananostructuredpolysaccharidebasedcolonspecificdrugdeliverysystemanapproachtoalternativecolonmedia AT webstertj anoveldissolutionmediafortestingdrugreleasefromananostructuredpolysaccharidebasedcolonspecificdrugdeliverysystemanapproachtoalternativecolonmedia AT kotlang noveldissolutionmediafortestingdrugreleasefromananostructuredpolysaccharidebasedcolonspecificdrugdeliverysystemanapproachtoalternativecolonmedia AT singhs noveldissolutionmediafortestingdrugreleasefromananostructuredpolysaccharidebasedcolonspecificdrugdeliverysystemanapproachtoalternativecolonmedia AT maddiboyinab noveldissolutionmediafortestingdrugreleasefromananostructuredpolysaccharidebasedcolonspecificdrugdeliverysystemanapproachtoalternativecolonmedia AT sunnapuo noveldissolutionmediafortestingdrugreleasefromananostructuredpolysaccharidebasedcolonspecificdrugdeliverysystemanapproachtoalternativecolonmedia AT webstertj noveldissolutionmediafortestingdrugreleasefromananostructuredpolysaccharidebasedcolonspecificdrugdeliverysystemanapproachtoalternativecolonmedia |
| _version_ |
1718400027175419904 |