Spatial Control of Neuronal Adhesion on Diamond-Like Carbon

This study reports a route to spatial control of neuronal adhesion onto Diamond-Like Carbon (DLC) by surface functionalisation by poly (oligo-ethyleneglycol methacrylate) (pOEGMA) and consequent laser ablation to produce cell adhesive tracks. DLC can be deposited as a tough and low friction coating...

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Autores principales:	James M Dugan, Carles Colominas, Andrés-Amador Garcia-Granada, Frederik Claeyssens
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	laser ablation diamond-like carbon poly(oligo (ethylene glycol) methacrylate) atom transfer radical polymerization neuronal cell culture Technology T
Acceso en línea:	https://doaj.org/article/f18cdaa5dcb249ada4f7fcab05eff92f
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id	oai:doaj.org-article:f18cdaa5dcb249ada4f7fcab05eff92f
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spelling	oai:doaj.org-article:f18cdaa5dcb249ada4f7fcab05eff92f2021-11-22T04:25:52ZSpatial Control of Neuronal Adhesion on Diamond-Like Carbon2296-801610.3389/fmats.2021.756055https://doaj.org/article/f18cdaa5dcb249ada4f7fcab05eff92f2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmats.2021.756055/fullhttps://doaj.org/toc/2296-8016This study reports a route to spatial control of neuronal adhesion onto Diamond-Like Carbon (DLC) by surface functionalisation by poly (oligo-ethyleneglycol methacrylate) (pOEGMA) and consequent laser ablation to produce cell adhesive tracks. DLC can be deposited as a tough and low friction coating on implantable devices and surgical instruments and has favourable properties for use as a biomaterial. The pOEGMA surface coating renders the DLC surface antifouling and the laser ablation creates graphitised tracks on the surface. The surfaces were coated with laminin, which adhered preferentially to the ablation tracks. The patterned surfaces were investigated for neuronal cell growth with NG108-15 cells for short term culture and rat neural stem cells for longer term culture. The cells initially adhered highly selectively to the ablation tracks while longer term cell culture revealed a more uniform cell coverage of the surface.James M DuganCarles ColominasAndrés-Amador Garcia-GranadaFrederik ClaeyssensFrontiers Media S.A.articlelaser ablationdiamond-like carbonpoly(oligo (ethylene glycol) methacrylate)atom transfer radical polymerizationneuronal cell cultureTechnologyTENFrontiers in Materials, Vol 8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	laser ablation diamond-like carbon poly(oligo (ethylene glycol) methacrylate) atom transfer radical polymerization neuronal cell culture Technology T
spellingShingle	laser ablation diamond-like carbon poly(oligo (ethylene glycol) methacrylate) atom transfer radical polymerization neuronal cell culture Technology T James M Dugan Carles Colominas Andrés-Amador Garcia-Granada Frederik Claeyssens Spatial Control of Neuronal Adhesion on Diamond-Like Carbon
description	This study reports a route to spatial control of neuronal adhesion onto Diamond-Like Carbon (DLC) by surface functionalisation by poly (oligo-ethyleneglycol methacrylate) (pOEGMA) and consequent laser ablation to produce cell adhesive tracks. DLC can be deposited as a tough and low friction coating on implantable devices and surgical instruments and has favourable properties for use as a biomaterial. The pOEGMA surface coating renders the DLC surface antifouling and the laser ablation creates graphitised tracks on the surface. The surfaces were coated with laminin, which adhered preferentially to the ablation tracks. The patterned surfaces were investigated for neuronal cell growth with NG108-15 cells for short term culture and rat neural stem cells for longer term culture. The cells initially adhered highly selectively to the ablation tracks while longer term cell culture revealed a more uniform cell coverage of the surface.
format	article
author	James M Dugan Carles Colominas Andrés-Amador Garcia-Granada Frederik Claeyssens
author_facet	James M Dugan Carles Colominas Andrés-Amador Garcia-Granada Frederik Claeyssens
author_sort	James M Dugan
title	Spatial Control of Neuronal Adhesion on Diamond-Like Carbon
title_short	Spatial Control of Neuronal Adhesion on Diamond-Like Carbon
title_full	Spatial Control of Neuronal Adhesion on Diamond-Like Carbon
title_fullStr	Spatial Control of Neuronal Adhesion on Diamond-Like Carbon
title_full_unstemmed	Spatial Control of Neuronal Adhesion on Diamond-Like Carbon
title_sort	spatial control of neuronal adhesion on diamond-like carbon
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/f18cdaa5dcb249ada4f7fcab05eff92f
work_keys_str_mv	AT jamesmdugan spatialcontrolofneuronaladhesionondiamondlikecarbon AT carlescolominas spatialcontrolofneuronaladhesionondiamondlikecarbon AT andresamadorgarciagranada spatialcontrolofneuronaladhesionondiamondlikecarbon AT frederikclaeyssens spatialcontrolofneuronaladhesionondiamondlikecarbon
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Spatial Control of Neuronal Adhesion on Diamond-Like Carbon

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