Development of a constant pressure perfused ex vivo model of the equine larynx.

Distal axonopathy is seen in a broad range of species including equine patients. In horses, this degenerative disorder of the recurrent laryngeal nerve is described as recurrent laryngeal neuropathy (RLN). The dysfunctional innervation of the cricoarytenoideus dorsalis muscle (CAD) leads to a loss o...

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Autores principales:	Sven Otto, Jule K Michler, Stefan Dhein, Christoph K W Mülling
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f196e68bd9b14c2daba0e2af63ffdcd6
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spelling	oai:doaj.org-article:f196e68bd9b14c2daba0e2af63ffdcd62021-11-25T06:23:46ZDevelopment of a constant pressure perfused ex vivo model of the equine larynx.1932-620310.1371/journal.pone.0251530https://doaj.org/article/f196e68bd9b14c2daba0e2af63ffdcd62021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0251530https://doaj.org/toc/1932-6203Distal axonopathy is seen in a broad range of species including equine patients. In horses, this degenerative disorder of the recurrent laryngeal nerve is described as recurrent laryngeal neuropathy (RLN). The dysfunctional innervation of the cricoarytenoideus dorsalis muscle (CAD) leads to a loss of performance in affected horses. In general, ex vivo models of the larynx are rare and for equine patients, just one short report is available. To allow for testing new therapy approaches in an isolated organ model, we examined equine larynges in a constant pressure perfused setup. In order to check the vitality and functionality of the isolated larynx, the vessels´ reaction to norepinephrine (NE) and sodium nitroprusside (NP) as vasoactive agents was tested. Additionally, the contractility of the CAD was checked via electrical stimulation. To determine the extent of hypoxic alterations, lactate dehydrogenase (LDH) and lactate were measured and an immunofluorescent analysis of hypoxia-inducible factor (HIF-1α), a key transcription factor in hypoxia, was performed. For this, a hypoxia-induced cell culture for HIF-1α was developed. The application of NE led to an expected vasoconstriction while NP caused the expected vasodilation. During a perfusion period of 352 ±20.78 min, LDH values were in the reference range and lactate values slightly exceeded the reference range at the end of the perfusion. HIF-1α nuclear translocation could reliably be detected in the hypoxia-induced cell cultures, but not in sections of the perfused CAD. With the approach presented here, a solid basis for perfusing equine larynges was established and may serve as a tool for further investigations of equine larynx disorders as well as a transferrable model for other species.Sven OttoJule K MichlerStefan DheinChristoph K W MüllingPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 5, p e0251530 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sven Otto Jule K Michler Stefan Dhein Christoph K W Mülling Development of a constant pressure perfused ex vivo model of the equine larynx.
description	Distal axonopathy is seen in a broad range of species including equine patients. In horses, this degenerative disorder of the recurrent laryngeal nerve is described as recurrent laryngeal neuropathy (RLN). The dysfunctional innervation of the cricoarytenoideus dorsalis muscle (CAD) leads to a loss of performance in affected horses. In general, ex vivo models of the larynx are rare and for equine patients, just one short report is available. To allow for testing new therapy approaches in an isolated organ model, we examined equine larynges in a constant pressure perfused setup. In order to check the vitality and functionality of the isolated larynx, the vessels´ reaction to norepinephrine (NE) and sodium nitroprusside (NP) as vasoactive agents was tested. Additionally, the contractility of the CAD was checked via electrical stimulation. To determine the extent of hypoxic alterations, lactate dehydrogenase (LDH) and lactate were measured and an immunofluorescent analysis of hypoxia-inducible factor (HIF-1α), a key transcription factor in hypoxia, was performed. For this, a hypoxia-induced cell culture for HIF-1α was developed. The application of NE led to an expected vasoconstriction while NP caused the expected vasodilation. During a perfusion period of 352 ±20.78 min, LDH values were in the reference range and lactate values slightly exceeded the reference range at the end of the perfusion. HIF-1α nuclear translocation could reliably be detected in the hypoxia-induced cell cultures, but not in sections of the perfused CAD. With the approach presented here, a solid basis for perfusing equine larynges was established and may serve as a tool for further investigations of equine larynx disorders as well as a transferrable model for other species.
format	article
author	Sven Otto Jule K Michler Stefan Dhein Christoph K W Mülling
author_facet	Sven Otto Jule K Michler Stefan Dhein Christoph K W Mülling
author_sort	Sven Otto
title	Development of a constant pressure perfused ex vivo model of the equine larynx.
title_short	Development of a constant pressure perfused ex vivo model of the equine larynx.
title_full	Development of a constant pressure perfused ex vivo model of the equine larynx.
title_fullStr	Development of a constant pressure perfused ex vivo model of the equine larynx.
title_full_unstemmed	Development of a constant pressure perfused ex vivo model of the equine larynx.
title_sort	development of a constant pressure perfused ex vivo model of the equine larynx.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/f196e68bd9b14c2daba0e2af63ffdcd6
work_keys_str_mv	AT svenotto developmentofaconstantpressureperfusedexvivomodeloftheequinelarynx AT julekmichler developmentofaconstantpressureperfusedexvivomodeloftheequinelarynx AT stefandhein developmentofaconstantpressureperfusedexvivomodeloftheequinelarynx AT christophkwmulling developmentofaconstantpressureperfusedexvivomodeloftheequinelarynx
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Development of a constant pressure perfused ex vivo model of the equine larynx.

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