Tracking antibiotic resistance genes and class 1 integrons in Escherichia coli isolates from wastewater and agricultural fields

Considering high concentrations of multidrug-resistant bacteria and antibiotic resistance genes (ARGs) in wastewater, agricultural reuse of treated wastewater may be a public health threat due to ARG dissemination in different environmental compartments, including soil and edible parts of crops. We...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Zahra Shamsizadeh, Mohammad Hassan Ehrampoush, Mahnaz Nikaeen, Mehdi Mokhtari, Mahsa Rahimi, Hossein Khanahmad, Farzaneh Mohammadi
Formato:	article
Lenguaje:	EN
Publicado:	IWA Publishing 2021
Materias:	antibiotic resistance escherichia coli integrons wastewater irrigation Environmental technology. Sanitary engineering TD1-1066
Acceso en línea:	https://doaj.org/article/f19d5f8e64c34527aa84ee7164474ad8
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f19d5f8e64c34527aa84ee7164474ad8
record_format	dspace
spelling	oai:doaj.org-article:f19d5f8e64c34527aa84ee7164474ad82021-11-06T11:20:14ZTracking antibiotic resistance genes and class 1 integrons in Escherichia coli isolates from wastewater and agricultural fields0273-12231996-973210.2166/wst.2021.288https://doaj.org/article/f19d5f8e64c34527aa84ee7164474ad82021-09-01T00:00:00Zhttp://wst.iwaponline.com/content/84/5/1182https://doaj.org/toc/0273-1223https://doaj.org/toc/1996-9732Considering high concentrations of multidrug-resistant bacteria and antibiotic resistance genes (ARGs) in wastewater, agricultural reuse of treated wastewater may be a public health threat due to ARG dissemination in different environmental compartments, including soil and edible parts of crops. We investigated the presence of antibiotic-resistant Escherichia coli as an indicator bacterium from secondary treated wastewater (STWW), water- or wastewater-irrigated soil and crop samples. ARGs including blaCTX-m-32, blaOXA-23, tet-W, sul1, cml-A, erm-B, along with intI1 gene in E. coli isolates were detected via molecular methods. The most prevalent ARGs in 78 E. coli isolates were sul1 (42%), followed by blaCTX-m-32 (19%), and erm-B (17%). IntI1 as a class 1 integrons gene was detected in 46% of the isolates. Cml-A was detected in STWW isolates but no E. coli isolate from wastewater-irrigated soil and crop samples contained this gene. The results also showed no detection of E. coli in water-irrigated soil and crop samples. Statistical analysis showed a correlation between sul1 and cml-A with intI1. The results suggest that agricultural reuse of wastewater may contribute to the transmission of antibiotic-resistant bacteria to soil and crop. Further research is needed to determine the potential risk of ARB associated with the consumption of wastewater-irrigated crops. HIGHLIGHTS Antibiotic-resistant E. coli presented different abundance in STWW, irrigated soil and crops.; Antibiotic resistance genes were detected in high numbers of E. coli isolates.; sul1 was the most abundant ARG in the E. coli isolates.; Wastewater irrigation could aggravate antibiotic resistance in soil and crops.;Zahra ShamsizadehMohammad Hassan EhrampoushMahnaz NikaeenMehdi MokhtariMahsa RahimiHossein KhanahmadFarzaneh MohammadiIWA Publishingarticleantibiotic resistanceescherichia coliintegronswastewater irrigationEnvironmental technology. Sanitary engineeringTD1-1066ENWater Science and Technology, Vol 84, Iss 5, Pp 1182-1189 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	antibiotic resistance escherichia coli integrons wastewater irrigation Environmental technology. Sanitary engineering TD1-1066
spellingShingle	antibiotic resistance escherichia coli integrons wastewater irrigation Environmental technology. Sanitary engineering TD1-1066 Zahra Shamsizadeh Mohammad Hassan Ehrampoush Mahnaz Nikaeen Mehdi Mokhtari Mahsa Rahimi Hossein Khanahmad Farzaneh Mohammadi Tracking antibiotic resistance genes and class 1 integrons in Escherichia coli isolates from wastewater and agricultural fields
description	Considering high concentrations of multidrug-resistant bacteria and antibiotic resistance genes (ARGs) in wastewater, agricultural reuse of treated wastewater may be a public health threat due to ARG dissemination in different environmental compartments, including soil and edible parts of crops. We investigated the presence of antibiotic-resistant Escherichia coli as an indicator bacterium from secondary treated wastewater (STWW), water- or wastewater-irrigated soil and crop samples. ARGs including blaCTX-m-32, blaOXA-23, tet-W, sul1, cml-A, erm-B, along with intI1 gene in E. coli isolates were detected via molecular methods. The most prevalent ARGs in 78 E. coli isolates were sul1 (42%), followed by blaCTX-m-32 (19%), and erm-B (17%). IntI1 as a class 1 integrons gene was detected in 46% of the isolates. Cml-A was detected in STWW isolates but no E. coli isolate from wastewater-irrigated soil and crop samples contained this gene. The results also showed no detection of E. coli in water-irrigated soil and crop samples. Statistical analysis showed a correlation between sul1 and cml-A with intI1. The results suggest that agricultural reuse of wastewater may contribute to the transmission of antibiotic-resistant bacteria to soil and crop. Further research is needed to determine the potential risk of ARB associated with the consumption of wastewater-irrigated crops. HIGHLIGHTS Antibiotic-resistant E. coli presented different abundance in STWW, irrigated soil and crops.; Antibiotic resistance genes were detected in high numbers of E. coli isolates.; sul1 was the most abundant ARG in the E. coli isolates.; Wastewater irrigation could aggravate antibiotic resistance in soil and crops.;
format	article
author	Zahra Shamsizadeh Mohammad Hassan Ehrampoush Mahnaz Nikaeen Mehdi Mokhtari Mahsa Rahimi Hossein Khanahmad Farzaneh Mohammadi
author_facet	Zahra Shamsizadeh Mohammad Hassan Ehrampoush Mahnaz Nikaeen Mehdi Mokhtari Mahsa Rahimi Hossein Khanahmad Farzaneh Mohammadi
author_sort	Zahra Shamsizadeh
title	Tracking antibiotic resistance genes and class 1 integrons in Escherichia coli isolates from wastewater and agricultural fields
title_short	Tracking antibiotic resistance genes and class 1 integrons in Escherichia coli isolates from wastewater and agricultural fields
title_full	Tracking antibiotic resistance genes and class 1 integrons in Escherichia coli isolates from wastewater and agricultural fields
title_fullStr	Tracking antibiotic resistance genes and class 1 integrons in Escherichia coli isolates from wastewater and agricultural fields
title_full_unstemmed	Tracking antibiotic resistance genes and class 1 integrons in Escherichia coli isolates from wastewater and agricultural fields
title_sort	tracking antibiotic resistance genes and class 1 integrons in escherichia coli isolates from wastewater and agricultural fields
publisher	IWA Publishing
publishDate	2021
url	https://doaj.org/article/f19d5f8e64c34527aa84ee7164474ad8
work_keys_str_mv	AT zahrashamsizadeh trackingantibioticresistancegenesandclass1integronsinescherichiacoliisolatesfromwastewaterandagriculturalfields AT mohammadhassanehrampoush trackingantibioticresistancegenesandclass1integronsinescherichiacoliisolatesfromwastewaterandagriculturalfields AT mahnaznikaeen trackingantibioticresistancegenesandclass1integronsinescherichiacoliisolatesfromwastewaterandagriculturalfields AT mehdimokhtari trackingantibioticresistancegenesandclass1integronsinescherichiacoliisolatesfromwastewaterandagriculturalfields AT mahsarahimi trackingantibioticresistancegenesandclass1integronsinescherichiacoliisolatesfromwastewaterandagriculturalfields AT hosseinkhanahmad trackingantibioticresistancegenesandclass1integronsinescherichiacoliisolatesfromwastewaterandagriculturalfields AT farzanehmohammadi trackingantibioticresistancegenesandclass1integronsinescherichiacoliisolatesfromwastewaterandagriculturalfields
_version_	1718443769727025152

Tracking antibiotic resistance genes and class 1 integrons in Escherichia coli isolates from wastewater and agricultural fields

Ejemplares similares