Preparation and characterization of a coacervate extended-release microparticulate delivery system for Lactobacillus rhamnosus

Sk Md Athar Alli Department of Pharmaceutical Technology, Jadavpur University, Kolkata, West Bengal, IndiaBackground: The purpose of this study was to develop a mucoadhesive coacervate microparticulate system to deliver viable Lactobacillus rhamnosus cells into the gut for an extended period of time...

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Autor principal:	Alli SMA
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/f1a2987932fa49c98846ac5c9f71d76c
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spelling	oai:doaj.org-article:f1a2987932fa49c98846ac5c9f71d76c2021-12-02T02:02:32ZPreparation and characterization of a coacervate extended-release microparticulate delivery system for Lactobacillus rhamnosus1176-91141178-2013https://doaj.org/article/f1a2987932fa49c98846ac5c9f71d76c2011-08-01T00:00:00Zhttp://www.dovepress.com/preparation-and-characterization-of-a-coacervate-extended-release-micr-a8117https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Sk Md Athar Alli Department of Pharmaceutical Technology, Jadavpur University, Kolkata, West Bengal, IndiaBackground: The purpose of this study was to develop a mucoadhesive coacervate microparticulate system to deliver viable Lactobacillus rhamnosus cells into the gut for an extended period of time while maintaining high numbers of viable cells within the formulation throughout its shelf-life and during gastrointestinal transit.Methods: Core coacervate mucoadhesive microparticles of L. rhamnosus were developed using several grades of hypromellose and were subsequently enteric-coated with hypromellose phthalate. Microparticles were evaluated for percent yield, entrapment efficiency, surface morphology, particle size, size distribution, zeta potential, flow properties, in vitro swelling, mucoadhesion properties, in vitro release profile and release kinetics, in vivo probiotic activity, and stability. The values for the kinetic constant and release exponent of model-dependent approaches, the difference factor, similarity factor, and Rescigno indices of model-independent approaches were determined for analyzing in vitro dissolution profiles.Results: Experimental microparticles of formulation batches were of spherical shape with percent yields of 41.24%&ndash;58.18%, entrapment efficiency 45.18%&ndash;64.16%, mean particle size 33.10&ndash;49.62 &micro;m, and zeta potential around -11.5 mV, confirming adequate stability of L. rhamnosus at room temperature. The in vitro L. rhamnosus release profile follows zero-order kinetics and depends on the grade of hypromellose and the L. rhamnosus to hypromellose ratio.Conclusion: Microparticles delivered L. rhamnosus in simulated intestinal conditions for an extended period, following zero-order kinetics, and exhibited appreciable mucoadhesion in simulated intestinal conditions.Keywords: Lactobacillus rhamnosus, mucoadhesive, microparticles, extended-release, intestineAlli SMADove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 1699-1707 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Alli SMA Preparation and characterization of a coacervate extended-release microparticulate delivery system for Lactobacillus rhamnosus
description	Sk Md Athar Alli Department of Pharmaceutical Technology, Jadavpur University, Kolkata, West Bengal, IndiaBackground: The purpose of this study was to develop a mucoadhesive coacervate microparticulate system to deliver viable Lactobacillus rhamnosus cells into the gut for an extended period of time while maintaining high numbers of viable cells within the formulation throughout its shelf-life and during gastrointestinal transit.Methods: Core coacervate mucoadhesive microparticles of L. rhamnosus were developed using several grades of hypromellose and were subsequently enteric-coated with hypromellose phthalate. Microparticles were evaluated for percent yield, entrapment efficiency, surface morphology, particle size, size distribution, zeta potential, flow properties, in vitro swelling, mucoadhesion properties, in vitro release profile and release kinetics, in vivo probiotic activity, and stability. The values for the kinetic constant and release exponent of model-dependent approaches, the difference factor, similarity factor, and Rescigno indices of model-independent approaches were determined for analyzing in vitro dissolution profiles.Results: Experimental microparticles of formulation batches were of spherical shape with percent yields of 41.24%&ndash;58.18%, entrapment efficiency 45.18%&ndash;64.16%, mean particle size 33.10&ndash;49.62 &micro;m, and zeta potential around -11.5 mV, confirming adequate stability of L. rhamnosus at room temperature. The in vitro L. rhamnosus release profile follows zero-order kinetics and depends on the grade of hypromellose and the L. rhamnosus to hypromellose ratio.Conclusion: Microparticles delivered L. rhamnosus in simulated intestinal conditions for an extended period, following zero-order kinetics, and exhibited appreciable mucoadhesion in simulated intestinal conditions.Keywords: Lactobacillus rhamnosus, mucoadhesive, microparticles, extended-release, intestine
format	article
author	Alli SMA
author_facet	Alli SMA
author_sort	Alli SMA
title	Preparation and characterization of a coacervate extended-release microparticulate delivery system for Lactobacillus rhamnosus
title_short	Preparation and characterization of a coacervate extended-release microparticulate delivery system for Lactobacillus rhamnosus
title_full	Preparation and characterization of a coacervate extended-release microparticulate delivery system for Lactobacillus rhamnosus
title_fullStr	Preparation and characterization of a coacervate extended-release microparticulate delivery system for Lactobacillus rhamnosus
title_full_unstemmed	Preparation and characterization of a coacervate extended-release microparticulate delivery system for Lactobacillus rhamnosus
title_sort	preparation and characterization of a coacervate extended-release microparticulate delivery system for lactobacillus rhamnosus
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/f1a2987932fa49c98846ac5c9f71d76c
work_keys_str_mv	AT allisma preparationandcharacterizationofacoacervateextendedreleasemicroparticulatedeliverysystemforlactobacillusrhamnosus
_version_	1718402747714240512

Preparation and characterization of a coacervate extended-release microparticulate delivery system for Lactobacillus rhamnosus

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