Distinct and Overlapping Functions of <i>Miscanthus sinensis</i> MYB Transcription Factors SCM1 and MYB103 in Lignin Biosynthesis

Cell wall recalcitrance is a major constraint for the exploitation of lignocellulosic biomass as a renewable resource for energy and bio-based products. Transcriptional regulators of the lignin biosynthetic pathway represent promising targets for tailoring lignin content and composition in plant sec...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Philippe Golfier, Olga Ermakova, Faride Unda, Emily K. Murphy, Jianbo Xie, Feng He, Wan Zhang, Jan U. Lohmann, Shawn D. Mansfield, Thomas Rausch, Sebastian Wolf
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	cell wall lignin biomass transcriptional regulation Miscanthus MYB Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/f1a4619d3dad4bcea67c373b1d3187d6
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f1a4619d3dad4bcea67c373b1d3187d6
record_format	dspace
spelling	oai:doaj.org-article:f1a4619d3dad4bcea67c373b1d3187d62021-11-25T17:56:15ZDistinct and Overlapping Functions of <i>Miscanthus sinensis</i> MYB Transcription Factors SCM1 and MYB103 in Lignin Biosynthesis10.3390/ijms2222123951422-00671661-6596https://doaj.org/article/f1a4619d3dad4bcea67c373b1d3187d62021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12395https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Cell wall recalcitrance is a major constraint for the exploitation of lignocellulosic biomass as a renewable resource for energy and bio-based products. Transcriptional regulators of the lignin biosynthetic pathway represent promising targets for tailoring lignin content and composition in plant secondary cell walls. However, knowledge about the transcriptional regulation of lignin biosynthesis in lignocellulosic feedstocks, such as Miscanthus, is limited. In Miscanthus leaves, <i>MsSCM1</i> and <i>MsMYB103</i> are expressed at growth stages associated with lignification. The ectopic expression of <i>MsSCM1</i> and <i>MsMYB103</i> in <i>N. benthamiana</i> leaves was sufficient to trigger secondary cell wall deposition with distinct sugar and lignin compositions. Moreover, RNA-seq analysis revealed that the transcriptional responses to <i>MsSCM1</i> and <i>MsMYB103</i> overexpression showed an extensive overlap with the response to the NAC master transcription factor MsSND1, but were distinct from each other, underscoring the inherent complexity of secondary cell wall formation. Furthermore, conserved and previously described promoter elements as well as novel and specific motifs could be identified from the target genes of the three transcription factors. Together, MsSCM1 and MsMYB103 represent interesting targets for manipulations of lignin content and composition in Miscanthus towards a tailored biomass.Philippe GolfierOlga ErmakovaFaride UndaEmily K. MurphyJianbo XieFeng HeWan ZhangJan U. LohmannShawn D. MansfieldThomas RauschSebastian WolfMDPI AGarticlecell wallligninbiomasstranscriptional regulationMiscanthusMYBBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12395, p 12395 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cell wall lignin biomass transcriptional regulation Miscanthus MYB Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	cell wall lignin biomass transcriptional regulation Miscanthus MYB Biology (General) QH301-705.5 Chemistry QD1-999 Philippe Golfier Olga Ermakova Faride Unda Emily K. Murphy Jianbo Xie Feng He Wan Zhang Jan U. Lohmann Shawn D. Mansfield Thomas Rausch Sebastian Wolf Distinct and Overlapping Functions of <i>Miscanthus sinensis</i> MYB Transcription Factors SCM1 and MYB103 in Lignin Biosynthesis
description	Cell wall recalcitrance is a major constraint for the exploitation of lignocellulosic biomass as a renewable resource for energy and bio-based products. Transcriptional regulators of the lignin biosynthetic pathway represent promising targets for tailoring lignin content and composition in plant secondary cell walls. However, knowledge about the transcriptional regulation of lignin biosynthesis in lignocellulosic feedstocks, such as Miscanthus, is limited. In Miscanthus leaves, <i>MsSCM1</i> and <i>MsMYB103</i> are expressed at growth stages associated with lignification. The ectopic expression of <i>MsSCM1</i> and <i>MsMYB103</i> in <i>N. benthamiana</i> leaves was sufficient to trigger secondary cell wall deposition with distinct sugar and lignin compositions. Moreover, RNA-seq analysis revealed that the transcriptional responses to <i>MsSCM1</i> and <i>MsMYB103</i> overexpression showed an extensive overlap with the response to the NAC master transcription factor MsSND1, but were distinct from each other, underscoring the inherent complexity of secondary cell wall formation. Furthermore, conserved and previously described promoter elements as well as novel and specific motifs could be identified from the target genes of the three transcription factors. Together, MsSCM1 and MsMYB103 represent interesting targets for manipulations of lignin content and composition in Miscanthus towards a tailored biomass.
format	article
author	Philippe Golfier Olga Ermakova Faride Unda Emily K. Murphy Jianbo Xie Feng He Wan Zhang Jan U. Lohmann Shawn D. Mansfield Thomas Rausch Sebastian Wolf
author_facet	Philippe Golfier Olga Ermakova Faride Unda Emily K. Murphy Jianbo Xie Feng He Wan Zhang Jan U. Lohmann Shawn D. Mansfield Thomas Rausch Sebastian Wolf
author_sort	Philippe Golfier
title	Distinct and Overlapping Functions of <i>Miscanthus sinensis</i> MYB Transcription Factors SCM1 and MYB103 in Lignin Biosynthesis
title_short	Distinct and Overlapping Functions of <i>Miscanthus sinensis</i> MYB Transcription Factors SCM1 and MYB103 in Lignin Biosynthesis
title_full	Distinct and Overlapping Functions of <i>Miscanthus sinensis</i> MYB Transcription Factors SCM1 and MYB103 in Lignin Biosynthesis
title_fullStr	Distinct and Overlapping Functions of <i>Miscanthus sinensis</i> MYB Transcription Factors SCM1 and MYB103 in Lignin Biosynthesis
title_full_unstemmed	Distinct and Overlapping Functions of <i>Miscanthus sinensis</i> MYB Transcription Factors SCM1 and MYB103 in Lignin Biosynthesis
title_sort	distinct and overlapping functions of <i>miscanthus sinensis</i> myb transcription factors scm1 and myb103 in lignin biosynthesis
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/f1a4619d3dad4bcea67c373b1d3187d6
work_keys_str_mv	AT philippegolfier distinctandoverlappingfunctionsofimiscanthussinensisimybtranscriptionfactorsscm1andmyb103inligninbiosynthesis AT olgaermakova distinctandoverlappingfunctionsofimiscanthussinensisimybtranscriptionfactorsscm1andmyb103inligninbiosynthesis AT farideunda distinctandoverlappingfunctionsofimiscanthussinensisimybtranscriptionfactorsscm1andmyb103inligninbiosynthesis AT emilykmurphy distinctandoverlappingfunctionsofimiscanthussinensisimybtranscriptionfactorsscm1andmyb103inligninbiosynthesis AT jianboxie distinctandoverlappingfunctionsofimiscanthussinensisimybtranscriptionfactorsscm1andmyb103inligninbiosynthesis AT fenghe distinctandoverlappingfunctionsofimiscanthussinensisimybtranscriptionfactorsscm1andmyb103inligninbiosynthesis AT wanzhang distinctandoverlappingfunctionsofimiscanthussinensisimybtranscriptionfactorsscm1andmyb103inligninbiosynthesis AT janulohmann distinctandoverlappingfunctionsofimiscanthussinensisimybtranscriptionfactorsscm1andmyb103inligninbiosynthesis AT shawndmansfield distinctandoverlappingfunctionsofimiscanthussinensisimybtranscriptionfactorsscm1andmyb103inligninbiosynthesis AT thomasrausch distinctandoverlappingfunctionsofimiscanthussinensisimybtranscriptionfactorsscm1andmyb103inligninbiosynthesis AT sebastianwolf distinctandoverlappingfunctionsofimiscanthussinensisimybtranscriptionfactorsscm1andmyb103inligninbiosynthesis
_version_	1718411839032786944

Distinct and Overlapping Functions of <i>Miscanthus sinensis</i> MYB Transcription Factors SCM1 and MYB103 in Lignin Biosynthesis

Ejemplares similares