Development of a chemically defined medium for Planctopirus limnophila to increase biomass production

Background: Planctomycetes is a phylum of biofilm-forming bacteria with numerous biosynthetic gene clusters, offering a promising source of new bioactive secondary metabolites. However, the current generation of chemically defined media achieves only low biomass yields, hindering research on these s...

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Autores principales:	Oscar Claudius Kruppa, Doreen Gerlach, Rong Fan, Peter Czermak
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Amino acid analysis Biomass Chemically defined medium Fed-batch cultivation Planctomycetes Planctopirus limnophila Biotechnology TP248.13-248.65 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/f1a70f3c551649fdb40fd97e16551c06
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spelling	oai:doaj.org-article:f1a70f3c551649fdb40fd97e16551c062021-11-06T04:20:36ZDevelopment of a chemically defined medium for Planctopirus limnophila to increase biomass production0717-345810.1016/j.ejbt.2021.09.002https://doaj.org/article/f1a70f3c551649fdb40fd97e16551c062021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0717345821000452https://doaj.org/toc/0717-3458Background: Planctomycetes is a phylum of biofilm-forming bacteria with numerous biosynthetic gene clusters, offering a promising source of new bioactive secondary metabolites. However, the current generation of chemically defined media achieves only low biomass yields, hindering research on these species. We therefore developed a chemically defined medium for the model organism Planctopirus limnophila to increase biomass production. Results: We found that P. limnophila grows best with a 10 mM sodium phosphate buffer. The replacement of complex nitrogen sources with defined amino acid solutions did not inhibit growth. Screening for vitamin requirements revealed that only cyanocobalamin (B12) is needed for growth. We used response surface methodology to optimize the medium, resulting in concentrations of 10 g/L glucose, 34 mL/L Hutner’s basal salts, 23.18 mM KNO3, 2.318 mM NH4Cl and 0.02 mg/L cyanocobalamin. The analysis of amino acid consumption allowed us to develop a customized amino acid solution lacking six of the amino acids present in Aminoplasmal 10%. Fed-batch cultivation in a bioreactor using the optimized medium achieved a final ΔOD600 of 46.8 ± 0.5 after 108 h, corresponding to a cell dry weight of 13.6 ± 0.7 g/L. Conclusions: The optimized chemically defined medium allowed us to produce larger amounts of biomass more quickly than reported in earlier studies. Further research should focus on triggering P. limnophila biofilm formation to activate the gene clusters responsible for secondary metabolism.How to cite: Kruppa OC, Gerlach D, Fan R, et al. Development of a chemically defined medium for Planctopirus limnophila to increase biomass production. Electron J Biotechnol 2021;54. https://doi.org/10.1016/j.ejbt.2021.09.002.Oscar Claudius KruppaDoreen GerlachRong FanPeter CzermakElsevierarticleAmino acid analysisBiomassChemically defined mediumFed-batch cultivationPlanctomycetesPlanctopirus limnophilaBiotechnologyTP248.13-248.65Biology (General)QH301-705.5ENElectronic Journal of Biotechnology, Vol 54, Iss , Pp 86-93 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Amino acid analysis Biomass Chemically defined medium Fed-batch cultivation Planctomycetes Planctopirus limnophila Biotechnology TP248.13-248.65 Biology (General) QH301-705.5
spellingShingle	Amino acid analysis Biomass Chemically defined medium Fed-batch cultivation Planctomycetes Planctopirus limnophila Biotechnology TP248.13-248.65 Biology (General) QH301-705.5 Oscar Claudius Kruppa Doreen Gerlach Rong Fan Peter Czermak Development of a chemically defined medium for Planctopirus limnophila to increase biomass production
description	Background: Planctomycetes is a phylum of biofilm-forming bacteria with numerous biosynthetic gene clusters, offering a promising source of new bioactive secondary metabolites. However, the current generation of chemically defined media achieves only low biomass yields, hindering research on these species. We therefore developed a chemically defined medium for the model organism Planctopirus limnophila to increase biomass production. Results: We found that P. limnophila grows best with a 10 mM sodium phosphate buffer. The replacement of complex nitrogen sources with defined amino acid solutions did not inhibit growth. Screening for vitamin requirements revealed that only cyanocobalamin (B12) is needed for growth. We used response surface methodology to optimize the medium, resulting in concentrations of 10 g/L glucose, 34 mL/L Hutner’s basal salts, 23.18 mM KNO3, 2.318 mM NH4Cl and 0.02 mg/L cyanocobalamin. The analysis of amino acid consumption allowed us to develop a customized amino acid solution lacking six of the amino acids present in Aminoplasmal 10%. Fed-batch cultivation in a bioreactor using the optimized medium achieved a final ΔOD600 of 46.8 ± 0.5 after 108 h, corresponding to a cell dry weight of 13.6 ± 0.7 g/L. Conclusions: The optimized chemically defined medium allowed us to produce larger amounts of biomass more quickly than reported in earlier studies. Further research should focus on triggering P. limnophila biofilm formation to activate the gene clusters responsible for secondary metabolism.How to cite: Kruppa OC, Gerlach D, Fan R, et al. Development of a chemically defined medium for Planctopirus limnophila to increase biomass production. Electron J Biotechnol 2021;54. https://doi.org/10.1016/j.ejbt.2021.09.002.
format	article
author	Oscar Claudius Kruppa Doreen Gerlach Rong Fan Peter Czermak
author_facet	Oscar Claudius Kruppa Doreen Gerlach Rong Fan Peter Czermak
author_sort	Oscar Claudius Kruppa
title	Development of a chemically defined medium for Planctopirus limnophila to increase biomass production
title_short	Development of a chemically defined medium for Planctopirus limnophila to increase biomass production
title_full	Development of a chemically defined medium for Planctopirus limnophila to increase biomass production
title_fullStr	Development of a chemically defined medium for Planctopirus limnophila to increase biomass production
title_full_unstemmed	Development of a chemically defined medium for Planctopirus limnophila to increase biomass production
title_sort	development of a chemically defined medium for planctopirus limnophila to increase biomass production
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/f1a70f3c551649fdb40fd97e16551c06
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_version_	1718443953688150016

Development of a chemically defined medium for Planctopirus limnophila to increase biomass production

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