Enhanced generation of induced pluripotent stem cells from a subpopulation of human fibroblasts.

<h4>Background</h4>The derivation of induced pluripotent stem cells (iPSCs) provides new possibilities for basic research and novel cell-based therapies. Limitations, however, include our current lack of understanding regarding the underlying mechanisms and the inefficiency of reprogramm...

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Autores principales:	James A Byrne, Ha Nam Nguyen, Renee A Reijo Pera
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f1b7c55831e14656a5e3e502525b2462
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spelling	oai:doaj.org-article:f1b7c55831e14656a5e3e502525b24622021-11-25T06:20:12ZEnhanced generation of induced pluripotent stem cells from a subpopulation of human fibroblasts.1932-620310.1371/journal.pone.0007118https://doaj.org/article/f1b7c55831e14656a5e3e502525b24622009-09-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19774082/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>The derivation of induced pluripotent stem cells (iPSCs) provides new possibilities for basic research and novel cell-based therapies. Limitations, however, include our current lack of understanding regarding the underlying mechanisms and the inefficiency of reprogramming.<h4>Methodology/principal findings</h4>Here, we report identification and isolation of a subpopulation of human dermal fibroblasts that express the pluripotency marker stage specific embryonic antigen 3 (SSEA3). Fibroblasts that expressed SSEA3 demonstrated an enhanced iPSC generation efficiency, while no iPSC derivation was obtained from the fibroblasts that did not express SSEA3. Transcriptional analysis revealed NANOG expression was significantly increased in the SSEA3 expressing fibroblasts, suggesting a possible mechanistic explanation for the differential reprogramming.<h4>Conclusions/significance</h4>To our knowledge, this study is the first to identify a pluripotency marker in a heterogeneous population of human dermal fibroblasts, to isolate a subpopulation of cells that have a significantly increased propensity to reprogram to pluripotency and to identify a possible mechanism to explain this differential reprogramming. This discovery provides a method to significantly increase the efficiency of reprogramming, enhancing the feasibility of the potential applications based on this technology, and a tool for basic research studies to understand the underlying reprogramming mechanisms.James A ByrneHa Nam NguyenRenee A Reijo PeraPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 9, p e7118 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q James A Byrne Ha Nam Nguyen Renee A Reijo Pera Enhanced generation of induced pluripotent stem cells from a subpopulation of human fibroblasts.
description	<h4>Background</h4>The derivation of induced pluripotent stem cells (iPSCs) provides new possibilities for basic research and novel cell-based therapies. Limitations, however, include our current lack of understanding regarding the underlying mechanisms and the inefficiency of reprogramming.<h4>Methodology/principal findings</h4>Here, we report identification and isolation of a subpopulation of human dermal fibroblasts that express the pluripotency marker stage specific embryonic antigen 3 (SSEA3). Fibroblasts that expressed SSEA3 demonstrated an enhanced iPSC generation efficiency, while no iPSC derivation was obtained from the fibroblasts that did not express SSEA3. Transcriptional analysis revealed NANOG expression was significantly increased in the SSEA3 expressing fibroblasts, suggesting a possible mechanistic explanation for the differential reprogramming.<h4>Conclusions/significance</h4>To our knowledge, this study is the first to identify a pluripotency marker in a heterogeneous population of human dermal fibroblasts, to isolate a subpopulation of cells that have a significantly increased propensity to reprogram to pluripotency and to identify a possible mechanism to explain this differential reprogramming. This discovery provides a method to significantly increase the efficiency of reprogramming, enhancing the feasibility of the potential applications based on this technology, and a tool for basic research studies to understand the underlying reprogramming mechanisms.
format	article
author	James A Byrne Ha Nam Nguyen Renee A Reijo Pera
author_facet	James A Byrne Ha Nam Nguyen Renee A Reijo Pera
author_sort	James A Byrne
title	Enhanced generation of induced pluripotent stem cells from a subpopulation of human fibroblasts.
title_short	Enhanced generation of induced pluripotent stem cells from a subpopulation of human fibroblasts.
title_full	Enhanced generation of induced pluripotent stem cells from a subpopulation of human fibroblasts.
title_fullStr	Enhanced generation of induced pluripotent stem cells from a subpopulation of human fibroblasts.
title_full_unstemmed	Enhanced generation of induced pluripotent stem cells from a subpopulation of human fibroblasts.
title_sort	enhanced generation of induced pluripotent stem cells from a subpopulation of human fibroblasts.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/f1b7c55831e14656a5e3e502525b2462
work_keys_str_mv	AT jamesabyrne enhancedgenerationofinducedpluripotentstemcellsfromasubpopulationofhumanfibroblasts AT hanamnguyen enhancedgenerationofinducedpluripotentstemcellsfromasubpopulationofhumanfibroblasts AT reneeareijopera enhancedgenerationofinducedpluripotentstemcellsfromasubpopulationofhumanfibroblasts
_version_	1718413837097500672

Enhanced generation of induced pluripotent stem cells from a subpopulation of human fibroblasts.

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