Integrated expression profiling and genome-wide analysis of ChREBP targets reveals the dual role for ChREBP in glucose-regulated gene expression.

The carbohydrate response element binding protein (ChREBP), a basic helix-loop-helix/leucine zipper transcription factor, plays a critical role in the control of lipogenesis in the liver. To identify the direct targets of ChREBP on a genome-wide scale and provide more insight into the mechanism by w...

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Autores principales:	Yun-Seung Jeong, Deokhoon Kim, Yong Seok Lee, Ha-Jung Kim, Jung-Youn Han, Seung-Soon Im, Hansook Kim Chong, Je-Keun Kwon, Yun-Ho Cho, Woo Kyung Kim, Timothy F Osborne, Jay D Horton, Hee-Sook Jun, Yong-Ho Ahn, Sung-Min Ahn, Ji-Young Cha
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f1c52acef876433e813933db620b2f52
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spelling	oai:doaj.org-article:f1c52acef876433e813933db620b2f522021-11-18T06:49:46ZIntegrated expression profiling and genome-wide analysis of ChREBP targets reveals the dual role for ChREBP in glucose-regulated gene expression.1932-620310.1371/journal.pone.0022544https://doaj.org/article/f1c52acef876433e813933db620b2f522011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21811631/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The carbohydrate response element binding protein (ChREBP), a basic helix-loop-helix/leucine zipper transcription factor, plays a critical role in the control of lipogenesis in the liver. To identify the direct targets of ChREBP on a genome-wide scale and provide more insight into the mechanism by which ChREBP regulates glucose-responsive gene expression, we performed chromatin immunoprecipitation-sequencing and gene expression analysis. We identified 1153 ChREBP binding sites and 783 target genes using the chromatin from HepG2, a human hepatocellular carcinoma cell line. A motif search revealed a refined consensus sequence (CABGTG-nnCnG-nGnSTG) to better represent critical elements of a functional ChREBP binding sequence. Gene ontology analysis shows that ChREBP target genes are particularly associated with lipid, fatty acid and steroid metabolism. In addition, other functional gene clusters related to transport, development and cell motility are significantly enriched. Gene set enrichment analysis reveals that ChREBP target genes are highly correlated with genes regulated by high glucose, providing a functional relevance to the genome-wide binding study. Furthermore, we have demonstrated that ChREBP may function as a transcriptional repressor as well as an activator.Yun-Seung JeongDeokhoon KimYong Seok LeeHa-Jung KimJung-Youn HanSeung-Soon ImHansook Kim ChongJe-Keun KwonYun-Ho ChoWoo Kyung KimTimothy F OsborneJay D HortonHee-Sook JunYong-Ho AhnSung-Min AhnJi-Young ChaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 7, p e22544 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yun-Seung Jeong Deokhoon Kim Yong Seok Lee Ha-Jung Kim Jung-Youn Han Seung-Soon Im Hansook Kim Chong Je-Keun Kwon Yun-Ho Cho Woo Kyung Kim Timothy F Osborne Jay D Horton Hee-Sook Jun Yong-Ho Ahn Sung-Min Ahn Ji-Young Cha Integrated expression profiling and genome-wide analysis of ChREBP targets reveals the dual role for ChREBP in glucose-regulated gene expression.
description	The carbohydrate response element binding protein (ChREBP), a basic helix-loop-helix/leucine zipper transcription factor, plays a critical role in the control of lipogenesis in the liver. To identify the direct targets of ChREBP on a genome-wide scale and provide more insight into the mechanism by which ChREBP regulates glucose-responsive gene expression, we performed chromatin immunoprecipitation-sequencing and gene expression analysis. We identified 1153 ChREBP binding sites and 783 target genes using the chromatin from HepG2, a human hepatocellular carcinoma cell line. A motif search revealed a refined consensus sequence (CABGTG-nnCnG-nGnSTG) to better represent critical elements of a functional ChREBP binding sequence. Gene ontology analysis shows that ChREBP target genes are particularly associated with lipid, fatty acid and steroid metabolism. In addition, other functional gene clusters related to transport, development and cell motility are significantly enriched. Gene set enrichment analysis reveals that ChREBP target genes are highly correlated with genes regulated by high glucose, providing a functional relevance to the genome-wide binding study. Furthermore, we have demonstrated that ChREBP may function as a transcriptional repressor as well as an activator.
format	article
author	Yun-Seung Jeong Deokhoon Kim Yong Seok Lee Ha-Jung Kim Jung-Youn Han Seung-Soon Im Hansook Kim Chong Je-Keun Kwon Yun-Ho Cho Woo Kyung Kim Timothy F Osborne Jay D Horton Hee-Sook Jun Yong-Ho Ahn Sung-Min Ahn Ji-Young Cha
author_facet	Yun-Seung Jeong Deokhoon Kim Yong Seok Lee Ha-Jung Kim Jung-Youn Han Seung-Soon Im Hansook Kim Chong Je-Keun Kwon Yun-Ho Cho Woo Kyung Kim Timothy F Osborne Jay D Horton Hee-Sook Jun Yong-Ho Ahn Sung-Min Ahn Ji-Young Cha
author_sort	Yun-Seung Jeong
title	Integrated expression profiling and genome-wide analysis of ChREBP targets reveals the dual role for ChREBP in glucose-regulated gene expression.
title_short	Integrated expression profiling and genome-wide analysis of ChREBP targets reveals the dual role for ChREBP in glucose-regulated gene expression.
title_full	Integrated expression profiling and genome-wide analysis of ChREBP targets reveals the dual role for ChREBP in glucose-regulated gene expression.
title_fullStr	Integrated expression profiling and genome-wide analysis of ChREBP targets reveals the dual role for ChREBP in glucose-regulated gene expression.
title_full_unstemmed	Integrated expression profiling and genome-wide analysis of ChREBP targets reveals the dual role for ChREBP in glucose-regulated gene expression.
title_sort	integrated expression profiling and genome-wide analysis of chrebp targets reveals the dual role for chrebp in glucose-regulated gene expression.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/f1c52acef876433e813933db620b2f52
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Integrated expression profiling and genome-wide analysis of ChREBP targets reveals the dual role for ChREBP in glucose-regulated gene expression.

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