Liraglutide Attenuates Aβ42 Generation in APPswe/SH-SY5Y Cells Through the Regulation of Autophagy
Jingjing Kong,1,* Liping Wan,2,* Yanfu Wang,1 Hua Zhang,1 Wei Zhang1 1Department of Geriatrics, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning Province, People’s Republic of China; 2Department of Clinical Laboratory, The First Affiliated Hospital of Dalian Med...
Guardado en:
| Autores principales: | , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Dove Medical Press
2020
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/f1c592ba597f4720ac5a9da19d0f0a5e |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:f1c592ba597f4720ac5a9da19d0f0a5e |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:f1c592ba597f4720ac5a9da19d0f0a5e2021-12-02T03:46:00ZLiraglutide Attenuates Aβ42 Generation in APPswe/SH-SY5Y Cells Through the Regulation of Autophagy1178-2021https://doaj.org/article/f1c592ba597f4720ac5a9da19d0f0a5e2020-07-01T00:00:00Zhttps://www.dovepress.com/liraglutide-attenuates-abeta42-generation-in-appswesh-sy5y-cells-throu-peer-reviewed-article-NDThttps://doaj.org/toc/1178-2021Jingjing Kong,1,* Liping Wan,2,* Yanfu Wang,1 Hua Zhang,1 Wei Zhang1 1Department of Geriatrics, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning Province, People’s Republic of China; 2Department of Clinical Laboratory, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning Province, People’s Republic of China*These authors contributed equally to this workCorrespondence: Jingjing Kong Tel +86-18098875886Email jingjingeasy@126.comObjective: This study aimed to clarify whether liraglutide, a GLP-1 analogue, can ameliorate Aβ pathology through the regulation of autophagy in Alzheimer’s disease (AD) and to explore the related mechanisms thereof.Methods: We used SH-SY5Y cells transiently transfected with APP695swe plasmid as an AD cellular model. Transfected cells were treated with liraglutide for 24 h in the presence or absence of 3-MA. Autophagy markers and the Aβ level were then evaluated by Western blot and ELISA. We also investigated the potential involvement of mTOR and JNK pathway in liraglutide-mediated autophagy.Results: Our results showed that liraglutide reduced Aβ 42 generation and enhanced autophagy in APPswe/SH-SY5Y cells; however, these effects could be counteracted with 3-MA. Furthermore, our data showed that liraglutide-induced autophagy does not follow the mTOR pathway. Liraglutide might promote autophagy in APPswe/SH-SY5Y cells by activating the JNK pathway and inhibiting the beclin-1/bcl-2 complex.Conclusion: Here, we report a novel mechanism underlying liraglutide-attenuated Aβ 42 generation through the activation of autophagy in AD cellular model.Keywords: Alzheimer’s disease, glucagonlike peptide 1, autophagy, Aβ, JNKKong JWan LWang YZhang HZhang WDove Medical Pressarticlealzheimer’s diseaseglucagonlike peptide 1autophagyaβjnkNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571Neurology. Diseases of the nervous systemRC346-429ENNeuropsychiatric Disease and Treatment, Vol Volume 16, Pp 1817-1825 (2020) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
alzheimer’s disease glucagonlike peptide 1 autophagy aβ jnk Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429 |
| spellingShingle |
alzheimer’s disease glucagonlike peptide 1 autophagy aβ jnk Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429 Kong J Wan L Wang Y Zhang H Zhang W Liraglutide Attenuates Aβ42 Generation in APPswe/SH-SY5Y Cells Through the Regulation of Autophagy |
| description |
Jingjing Kong,1,* Liping Wan,2,* Yanfu Wang,1 Hua Zhang,1 Wei Zhang1 1Department of Geriatrics, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning Province, People’s Republic of China; 2Department of Clinical Laboratory, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning Province, People’s Republic of China*These authors contributed equally to this workCorrespondence: Jingjing Kong Tel +86-18098875886Email jingjingeasy@126.comObjective: This study aimed to clarify whether liraglutide, a GLP-1 analogue, can ameliorate Aβ pathology through the regulation of autophagy in Alzheimer’s disease (AD) and to explore the related mechanisms thereof.Methods: We used SH-SY5Y cells transiently transfected with APP695swe plasmid as an AD cellular model. Transfected cells were treated with liraglutide for 24 h in the presence or absence of 3-MA. Autophagy markers and the Aβ level were then evaluated by Western blot and ELISA. We also investigated the potential involvement of mTOR and JNK pathway in liraglutide-mediated autophagy.Results: Our results showed that liraglutide reduced Aβ 42 generation and enhanced autophagy in APPswe/SH-SY5Y cells; however, these effects could be counteracted with 3-MA. Furthermore, our data showed that liraglutide-induced autophagy does not follow the mTOR pathway. Liraglutide might promote autophagy in APPswe/SH-SY5Y cells by activating the JNK pathway and inhibiting the beclin-1/bcl-2 complex.Conclusion: Here, we report a novel mechanism underlying liraglutide-attenuated Aβ 42 generation through the activation of autophagy in AD cellular model.Keywords: Alzheimer’s disease, glucagonlike peptide 1, autophagy, Aβ, JNK |
| format |
article |
| author |
Kong J Wan L Wang Y Zhang H Zhang W |
| author_facet |
Kong J Wan L Wang Y Zhang H Zhang W |
| author_sort |
Kong J |
| title |
Liraglutide Attenuates Aβ42 Generation in APPswe/SH-SY5Y Cells Through the Regulation of Autophagy |
| title_short |
Liraglutide Attenuates Aβ42 Generation in APPswe/SH-SY5Y Cells Through the Regulation of Autophagy |
| title_full |
Liraglutide Attenuates Aβ42 Generation in APPswe/SH-SY5Y Cells Through the Regulation of Autophagy |
| title_fullStr |
Liraglutide Attenuates Aβ42 Generation in APPswe/SH-SY5Y Cells Through the Regulation of Autophagy |
| title_full_unstemmed |
Liraglutide Attenuates Aβ42 Generation in APPswe/SH-SY5Y Cells Through the Regulation of Autophagy |
| title_sort |
liraglutide attenuates aβ42 generation in appswe/sh-sy5y cells through the regulation of autophagy |
| publisher |
Dove Medical Press |
| publishDate |
2020 |
| url |
https://doaj.org/article/f1c592ba597f4720ac5a9da19d0f0a5e |
| work_keys_str_mv |
AT kongj liraglutideattenuatesabeta42generationinappsweshsy5ycellsthroughtheregulationofautophagy AT wanl liraglutideattenuatesabeta42generationinappsweshsy5ycellsthroughtheregulationofautophagy AT wangy liraglutideattenuatesabeta42generationinappsweshsy5ycellsthroughtheregulationofautophagy AT zhangh liraglutideattenuatesabeta42generationinappsweshsy5ycellsthroughtheregulationofautophagy AT zhangw liraglutideattenuatesabeta42generationinappsweshsy5ycellsthroughtheregulationofautophagy |
| _version_ |
1718401648699637760 |