DHA but not EPA induces the trans-differentiation of C2C12 cells into white-like adipocytes phenotype.

Muscle derived stem cells (MDSCs) and myoblast play an important role in myotube regeneration when muscle tissue is injured. However, these cells can be induced to differentiate into adipocytes once exposed to PPARγ activator like EPA and DHA that are highly suggested during pregnancy. The objective...

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Autores principales:	Saeed Ghnaimawi, Lisa Rebello, Jamie Baum, Yan Huang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f1c5ee6965fd4c52a17c0cac329f1279
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spelling	oai:doaj.org-article:f1c5ee6965fd4c52a17c0cac329f12792021-12-02T20:08:39ZDHA but not EPA induces the trans-differentiation of C2C12 cells into white-like adipocytes phenotype.1932-620310.1371/journal.pone.0249438https://doaj.org/article/f1c5ee6965fd4c52a17c0cac329f12792021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0249438https://doaj.org/toc/1932-6203Muscle derived stem cells (MDSCs) and myoblast play an important role in myotube regeneration when muscle tissue is injured. However, these cells can be induced to differentiate into adipocytes once exposed to PPARγ activator like EPA and DHA that are highly suggested during pregnancy. The objective of this study aims at determining the identity of trans-differentiated cells by exploring the effect of EPA and DHA on C2C12 undergoing differentiation into brown and white adipocytes. DHA but not EPA committed C2C12 cells reprograming into white like adipocyte phenotype. Also, DHA promoted the expression of lipolysis regulating genes but had no effect on genes regulating β-oxidation referring to its implication in lipid re-esterification. Furthermore, DHA impaired C2C12 cells differentiation into brown adipocytes through reducing the thermogenic capacity and mitochondrial biogenesis of derived cells independent of UCP1. Accordingly, DHA treated groups showed an increased accumulation of lipid droplets and suppressed mitochondrial maximal respiration and spare respiratory capacity. EPA, on the other hand, reduced myogenesis regulating genes, but no significant differences were observed in the expression of adipogenesis key genes. Likewise, EPA suppressed the expression of WAT signature genes indicating that EPA and DHA have an independent role on white adipogensis. Unlike DHA treatment, EPA supplementation had no effect on the differential of C2C12 cells into brown adipocytes. In conclusion, DHA is a potent adipogenic and lipogenic factor that can change the metabolic profile of muscle cells by increasing myocellular fat.Saeed GhnaimawiLisa RebelloJamie BaumYan HuangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 9, p e0249438 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Saeed Ghnaimawi Lisa Rebello Jamie Baum Yan Huang DHA but not EPA induces the trans-differentiation of C2C12 cells into white-like adipocytes phenotype.
description	Muscle derived stem cells (MDSCs) and myoblast play an important role in myotube regeneration when muscle tissue is injured. However, these cells can be induced to differentiate into adipocytes once exposed to PPARγ activator like EPA and DHA that are highly suggested during pregnancy. The objective of this study aims at determining the identity of trans-differentiated cells by exploring the effect of EPA and DHA on C2C12 undergoing differentiation into brown and white adipocytes. DHA but not EPA committed C2C12 cells reprograming into white like adipocyte phenotype. Also, DHA promoted the expression of lipolysis regulating genes but had no effect on genes regulating β-oxidation referring to its implication in lipid re-esterification. Furthermore, DHA impaired C2C12 cells differentiation into brown adipocytes through reducing the thermogenic capacity and mitochondrial biogenesis of derived cells independent of UCP1. Accordingly, DHA treated groups showed an increased accumulation of lipid droplets and suppressed mitochondrial maximal respiration and spare respiratory capacity. EPA, on the other hand, reduced myogenesis regulating genes, but no significant differences were observed in the expression of adipogenesis key genes. Likewise, EPA suppressed the expression of WAT signature genes indicating that EPA and DHA have an independent role on white adipogensis. Unlike DHA treatment, EPA supplementation had no effect on the differential of C2C12 cells into brown adipocytes. In conclusion, DHA is a potent adipogenic and lipogenic factor that can change the metabolic profile of muscle cells by increasing myocellular fat.
format	article
author	Saeed Ghnaimawi Lisa Rebello Jamie Baum Yan Huang
author_facet	Saeed Ghnaimawi Lisa Rebello Jamie Baum Yan Huang
author_sort	Saeed Ghnaimawi
title	DHA but not EPA induces the trans-differentiation of C2C12 cells into white-like adipocytes phenotype.
title_short	DHA but not EPA induces the trans-differentiation of C2C12 cells into white-like adipocytes phenotype.
title_full	DHA but not EPA induces the trans-differentiation of C2C12 cells into white-like adipocytes phenotype.
title_fullStr	DHA but not EPA induces the trans-differentiation of C2C12 cells into white-like adipocytes phenotype.
title_full_unstemmed	DHA but not EPA induces the trans-differentiation of C2C12 cells into white-like adipocytes phenotype.
title_sort	dha but not epa induces the trans-differentiation of c2c12 cells into white-like adipocytes phenotype.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/f1c5ee6965fd4c52a17c0cac329f1279
work_keys_str_mv	AT saeedghnaimawi dhabutnotepainducesthetransdifferentiationofc2c12cellsintowhitelikeadipocytesphenotype AT lisarebello dhabutnotepainducesthetransdifferentiationofc2c12cellsintowhitelikeadipocytesphenotype AT jamiebaum dhabutnotepainducesthetransdifferentiationofc2c12cellsintowhitelikeadipocytesphenotype AT yanhuang dhabutnotepainducesthetransdifferentiationofc2c12cellsintowhitelikeadipocytesphenotype
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DHA but not EPA induces the trans-differentiation of C2C12 cells into white-like adipocytes phenotype.

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