Morphological and immunohistochemical phenotype of TCs in the intestinal bulb of Grass carp and their potential role in intestinal immunity

Abstract The current study investigated telocytes (TCs) in the intestinal bulb of Grass carp using light microscopy (LM), Transmission electron microscopy (TEM), scanning electron microscopy, and immunohistochemistry (IHC). By LM, TCs were distinguished by the typical morphological features that had...

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Autores principales:	Hanan H. Abd-Elhafeez, Alaa S. Abou-Elhamd, Soha A. Soliman
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f1c617152ae94ae087fb1fbae2a3f767
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spelling	oai:doaj.org-article:f1c617152ae94ae087fb1fbae2a3f7672021-12-02T16:45:46ZMorphological and immunohistochemical phenotype of TCs in the intestinal bulb of Grass carp and their potential role in intestinal immunity10.1038/s41598-020-70032-y2045-2322https://doaj.org/article/f1c617152ae94ae087fb1fbae2a3f7672020-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-70032-yhttps://doaj.org/toc/2045-2322Abstract The current study investigated telocytes (TCs) in the intestinal bulb of Grass carp using light microscopy (LM), Transmission electron microscopy (TEM), scanning electron microscopy, and immunohistochemistry (IHC). By LM, TCs were distinguished by the typical morphological features that had a cell body and telopodes using HE, toluidine blue, methylene blue, Marsland silver stain, Grimelius’s silver nitrate, Giemsa, PAS, combined AB pH2,5/PAS, Crossmon’s and Mallory triple trichrome, Van Gieson stains, Verhoeff’s stain, Sudan black, osmic acid, performic acid with methylene blue and bromophenol blue. TCs were identified under the epithelium as an individual cell or formed a TCs sheath. They detected in the lamina propria, between muscle fibers, around the myenteric plexus and fibrous tissue. TCs acquired immunological features of endocrine cells that exhibited high affinity for silver stain, performic acid with methylene blue, Marsland stain, and immunohistochemical staining using chromogranin A. Sub epithelial TCs were closely related to the endocrine cells. TCs and their secretory activities were recognized using acridine orange. TCs were identified by IHC using CD34, CD117, S100-protein, desmin. TCs formed a3D network that established contact with macrophage, mast cells, dendritic cells, lymphocytes, smooth muscle fibers, fibroblast, Schwann cells and nerve fibers. In conclusion, the localization of TCs in relation to different types of immune cells indicated their potential role in the maintenance of intestinal immunity.Hanan H. Abd-ElhafeezAlaa S. Abou-ElhamdSoha A. SolimanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-17 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hanan H. Abd-Elhafeez Alaa S. Abou-Elhamd Soha A. Soliman Morphological and immunohistochemical phenotype of TCs in the intestinal bulb of Grass carp and their potential role in intestinal immunity
description	Abstract The current study investigated telocytes (TCs) in the intestinal bulb of Grass carp using light microscopy (LM), Transmission electron microscopy (TEM), scanning electron microscopy, and immunohistochemistry (IHC). By LM, TCs were distinguished by the typical morphological features that had a cell body and telopodes using HE, toluidine blue, methylene blue, Marsland silver stain, Grimelius’s silver nitrate, Giemsa, PAS, combined AB pH2,5/PAS, Crossmon’s and Mallory triple trichrome, Van Gieson stains, Verhoeff’s stain, Sudan black, osmic acid, performic acid with methylene blue and bromophenol blue. TCs were identified under the epithelium as an individual cell or formed a TCs sheath. They detected in the lamina propria, between muscle fibers, around the myenteric plexus and fibrous tissue. TCs acquired immunological features of endocrine cells that exhibited high affinity for silver stain, performic acid with methylene blue, Marsland stain, and immunohistochemical staining using chromogranin A. Sub epithelial TCs were closely related to the endocrine cells. TCs and their secretory activities were recognized using acridine orange. TCs were identified by IHC using CD34, CD117, S100-protein, desmin. TCs formed a3D network that established contact with macrophage, mast cells, dendritic cells, lymphocytes, smooth muscle fibers, fibroblast, Schwann cells and nerve fibers. In conclusion, the localization of TCs in relation to different types of immune cells indicated their potential role in the maintenance of intestinal immunity.
format	article
author	Hanan H. Abd-Elhafeez Alaa S. Abou-Elhamd Soha A. Soliman
author_facet	Hanan H. Abd-Elhafeez Alaa S. Abou-Elhamd Soha A. Soliman
author_sort	Hanan H. Abd-Elhafeez
title	Morphological and immunohistochemical phenotype of TCs in the intestinal bulb of Grass carp and their potential role in intestinal immunity
title_short	Morphological and immunohistochemical phenotype of TCs in the intestinal bulb of Grass carp and their potential role in intestinal immunity
title_full	Morphological and immunohistochemical phenotype of TCs in the intestinal bulb of Grass carp and their potential role in intestinal immunity
title_fullStr	Morphological and immunohistochemical phenotype of TCs in the intestinal bulb of Grass carp and their potential role in intestinal immunity
title_full_unstemmed	Morphological and immunohistochemical phenotype of TCs in the intestinal bulb of Grass carp and their potential role in intestinal immunity
title_sort	morphological and immunohistochemical phenotype of tcs in the intestinal bulb of grass carp and their potential role in intestinal immunity
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/f1c617152ae94ae087fb1fbae2a3f767
work_keys_str_mv	AT hananhabdelhafeez morphologicalandimmunohistochemicalphenotypeoftcsintheintestinalbulbofgrasscarpandtheirpotentialroleinintestinalimmunity AT alaasabouelhamd morphologicalandimmunohistochemicalphenotypeoftcsintheintestinalbulbofgrasscarpandtheirpotentialroleinintestinalimmunity AT sohaasoliman morphologicalandimmunohistochemicalphenotypeoftcsintheintestinalbulbofgrasscarpandtheirpotentialroleinintestinalimmunity
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Morphological and immunohistochemical phenotype of TCs in the intestinal bulb of Grass carp and their potential role in intestinal immunity

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