Ionising Radiation Promotes Invasive Potential of Breast Cancer Cells: The Role of Exosomes in the Process

Along with the cells that are exposed to radiation, non-irradiated cells can unveil radiation effects as a result of intercellular communication, which are collectively defined as radiation induced bystander effects (RIBE). Exosome-mediated signalling is one of the core mechanisms responsible for mu...

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Autores principales:	Raheem AL-Abedi, Seda Tuncay Cagatay, Ammar Mayah, Susan A. Brooks, Munira Kadhim
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	exosomes breast cancer RIBE invasiveness EMT glycosylation Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/f1c618a5587f4e73808c65024f80600a
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spelling	oai:doaj.org-article:f1c618a5587f4e73808c65024f80600a2021-11-11T17:03:14ZIonising Radiation Promotes Invasive Potential of Breast Cancer Cells: The Role of Exosomes in the Process10.3390/ijms2221115701422-00671661-6596https://doaj.org/article/f1c618a5587f4e73808c65024f80600a2021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11570https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Along with the cells that are exposed to radiation, non-irradiated cells can unveil radiation effects as a result of intercellular communication, which are collectively defined as radiation induced bystander effects (RIBE). Exosome-mediated signalling is one of the core mechanisms responsible for multidirectional communication of tumor cells and their associated microenvironment, which may result in enhancement of malignant tumor phenotypes. Recent studies show that exosomes and exosome-mediated signalling also play a dynamic role in RIBE in cancer cell lines, many of which focused on altered exosome cargo or their effects on DNA damage. However, there is a lack of knowledge regarding how these changes in exosome cargo are reflected in other functional characteristics of cancer cells from the aspects of invasiveness and metastasis. Therefore, in the current study, we aimed to investigate exosome-mediated bystander effects of 2 Gy X-ray therapeutic dose of ionizing radiation on the invasive potential of MCF-7 breast cancer cells in vitro via assessing Matrigel invasion potential, epithelial mesenchymal transition (EMT) characteristics and the extent of glycosylation, as well as underlying plausible molecular mechanisms. The findings show that exosomes derived from irradiated MCF-7 cells enhance invasiveness of bystander MCF-7 cells, possibly through altered miRNA and protein content carried in exosomes.Raheem AL-AbediSeda Tuncay CagatayAmmar MayahSusan A. BrooksMunira KadhimMDPI AGarticleexosomesbreast cancerRIBEinvasivenessEMTglycosylationBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11570, p 11570 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	exosomes breast cancer RIBE invasiveness EMT glycosylation Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	exosomes breast cancer RIBE invasiveness EMT glycosylation Biology (General) QH301-705.5 Chemistry QD1-999 Raheem AL-Abedi Seda Tuncay Cagatay Ammar Mayah Susan A. Brooks Munira Kadhim Ionising Radiation Promotes Invasive Potential of Breast Cancer Cells: The Role of Exosomes in the Process
description	Along with the cells that are exposed to radiation, non-irradiated cells can unveil radiation effects as a result of intercellular communication, which are collectively defined as radiation induced bystander effects (RIBE). Exosome-mediated signalling is one of the core mechanisms responsible for multidirectional communication of tumor cells and their associated microenvironment, which may result in enhancement of malignant tumor phenotypes. Recent studies show that exosomes and exosome-mediated signalling also play a dynamic role in RIBE in cancer cell lines, many of which focused on altered exosome cargo or their effects on DNA damage. However, there is a lack of knowledge regarding how these changes in exosome cargo are reflected in other functional characteristics of cancer cells from the aspects of invasiveness and metastasis. Therefore, in the current study, we aimed to investigate exosome-mediated bystander effects of 2 Gy X-ray therapeutic dose of ionizing radiation on the invasive potential of MCF-7 breast cancer cells in vitro via assessing Matrigel invasion potential, epithelial mesenchymal transition (EMT) characteristics and the extent of glycosylation, as well as underlying plausible molecular mechanisms. The findings show that exosomes derived from irradiated MCF-7 cells enhance invasiveness of bystander MCF-7 cells, possibly through altered miRNA and protein content carried in exosomes.
format	article
author	Raheem AL-Abedi Seda Tuncay Cagatay Ammar Mayah Susan A. Brooks Munira Kadhim
author_facet	Raheem AL-Abedi Seda Tuncay Cagatay Ammar Mayah Susan A. Brooks Munira Kadhim
author_sort	Raheem AL-Abedi
title	Ionising Radiation Promotes Invasive Potential of Breast Cancer Cells: The Role of Exosomes in the Process
title_short	Ionising Radiation Promotes Invasive Potential of Breast Cancer Cells: The Role of Exosomes in the Process
title_full	Ionising Radiation Promotes Invasive Potential of Breast Cancer Cells: The Role of Exosomes in the Process
title_fullStr	Ionising Radiation Promotes Invasive Potential of Breast Cancer Cells: The Role of Exosomes in the Process
title_full_unstemmed	Ionising Radiation Promotes Invasive Potential of Breast Cancer Cells: The Role of Exosomes in the Process
title_sort	ionising radiation promotes invasive potential of breast cancer cells: the role of exosomes in the process
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/f1c618a5587f4e73808c65024f80600a
work_keys_str_mv	AT raheemalabedi ionisingradiationpromotesinvasivepotentialofbreastcancercellstheroleofexosomesintheprocess AT sedatuncaycagatay ionisingradiationpromotesinvasivepotentialofbreastcancercellstheroleofexosomesintheprocess AT ammarmayah ionisingradiationpromotesinvasivepotentialofbreastcancercellstheroleofexosomesintheprocess AT susanabrooks ionisingradiationpromotesinvasivepotentialofbreastcancercellstheroleofexosomesintheprocess AT munirakadhim ionisingradiationpromotesinvasivepotentialofbreastcancercellstheroleofexosomesintheprocess
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Ionising Radiation Promotes Invasive Potential of Breast Cancer Cells: The Role of Exosomes in the Process

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