Interactions of PatA with the Divisome during Heterocyst Differentiation in <italic toggle="yes">Anabaena</italic>

ABSTRACT The Anabaena organismic unit is a filament of communicating cells. Under conditions of nitrogen scarcity, some cells along the filament differentiate into heterocysts, which are specialized in the fixation of atmospheric N2 and provide the vegetative cells with N2 fixation products. At a ce...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ana Valladares, Cristina Velázquez-Suárez, Antonia Herrero
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	Cyanobacteria bacterial multicellularity cell differentiation cell division Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/f1c68bde44624ab29444b2a4e6e2cd43
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f1c68bde44624ab29444b2a4e6e2cd43
record_format	dspace
spelling	oai:doaj.org-article:f1c68bde44624ab29444b2a4e6e2cd432021-11-15T15:30:14ZInteractions of PatA with the Divisome during Heterocyst Differentiation in <italic toggle="yes">Anabaena</italic>10.1128/mSphere.00188-202379-5042https://doaj.org/article/f1c68bde44624ab29444b2a4e6e2cd432020-06-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00188-20https://doaj.org/toc/2379-5042ABSTRACT The Anabaena organismic unit is a filament of communicating cells. Under conditions of nitrogen scarcity, some cells along the filament differentiate into heterocysts, which are specialized in the fixation of atmospheric N2 and provide the vegetative cells with N2 fixation products. At a certain stage, the differentiation process becomes irreversible, so that even when nitrogen is replenished, no return to the vegetative cell state takes place, possibly as a consequence of loss of cell division capacity. Upon N-stepdown, midcell FtsZ-rings were detected in vegetative cells, but not in differentiating cells, and this was also the case for ZipN, an essential protein that participates in FtsZ tethering to the cytoplasmic membrane and divisome organization. Later, expression of ftsZ was arrested in mature heterocysts. PatA is a protein required for the differentiation of intercalary heterocysts in Anabaena. The expression level of the patA gene was increased in differentiating cells, and a mutant strain lacking PatA exhibited enhanced FtsZ-rings. PatA was capable of direct interactions with ZipN and SepF, another essential component of the Anabaena Z-ring. Thus, PatA appears to promote inhibition of cell division in the differentiating cells, allowing progress of the differentiation process. PatA, which in mature heterocysts was detected at the cell poles, could interact also with SepJ, a protein involved in production of the septal junctions that provide cell-cell adhesion and intercellular communication in the filament, hinting at a further role of PatA in the formation or stability of the intercellular structures that are at the basis of the multicellular character of Anabaena. IMPORTANCE Anabaena is a cyanobacterial model that represents an ancient and simple form of biological multicellularity. The Anabaena organism is a filament of cohesive and communicating cells that can include cells specialized in different tasks. Thus, under conditions of nitrogen scarcity, certain cells of the filament differentiate into heterocysts, which fix atmospheric nitrogen and provide organic nitrogen to the rest of cells, which, in turn, provide heterocysts with organic carbon. Heterocyst differentiation involves extensive morphological, biochemical, and genetic changes, becoming irreversible at a certain stage. We studied the regulation during heterocyst differentiation of several essential components of the Anabaena cell division machinery and found that protein PatA, which is required for differentiation and is induced in differentiating cells, interacts with essential cell division factors and destabilizes the cell division complex. This suggests a mechanism for establishment of commitment to differentiation by inhibition of cell division.Ana ValladaresCristina Velázquez-SuárezAntonia HerreroAmerican Society for MicrobiologyarticleCyanobacteriabacterial multicellularitycell differentiationcell divisionMicrobiologyQR1-502ENmSphere, Vol 5, Iss 3 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cyanobacteria bacterial multicellularity cell differentiation cell division Microbiology QR1-502
spellingShingle	Cyanobacteria bacterial multicellularity cell differentiation cell division Microbiology QR1-502 Ana Valladares Cristina Velázquez-Suárez Antonia Herrero Interactions of PatA with the Divisome during Heterocyst Differentiation in <italic toggle="yes">Anabaena</italic>
description	ABSTRACT The Anabaena organismic unit is a filament of communicating cells. Under conditions of nitrogen scarcity, some cells along the filament differentiate into heterocysts, which are specialized in the fixation of atmospheric N2 and provide the vegetative cells with N2 fixation products. At a certain stage, the differentiation process becomes irreversible, so that even when nitrogen is replenished, no return to the vegetative cell state takes place, possibly as a consequence of loss of cell division capacity. Upon N-stepdown, midcell FtsZ-rings were detected in vegetative cells, but not in differentiating cells, and this was also the case for ZipN, an essential protein that participates in FtsZ tethering to the cytoplasmic membrane and divisome organization. Later, expression of ftsZ was arrested in mature heterocysts. PatA is a protein required for the differentiation of intercalary heterocysts in Anabaena. The expression level of the patA gene was increased in differentiating cells, and a mutant strain lacking PatA exhibited enhanced FtsZ-rings. PatA was capable of direct interactions with ZipN and SepF, another essential component of the Anabaena Z-ring. Thus, PatA appears to promote inhibition of cell division in the differentiating cells, allowing progress of the differentiation process. PatA, which in mature heterocysts was detected at the cell poles, could interact also with SepJ, a protein involved in production of the septal junctions that provide cell-cell adhesion and intercellular communication in the filament, hinting at a further role of PatA in the formation or stability of the intercellular structures that are at the basis of the multicellular character of Anabaena. IMPORTANCE Anabaena is a cyanobacterial model that represents an ancient and simple form of biological multicellularity. The Anabaena organism is a filament of cohesive and communicating cells that can include cells specialized in different tasks. Thus, under conditions of nitrogen scarcity, certain cells of the filament differentiate into heterocysts, which fix atmospheric nitrogen and provide organic nitrogen to the rest of cells, which, in turn, provide heterocysts with organic carbon. Heterocyst differentiation involves extensive morphological, biochemical, and genetic changes, becoming irreversible at a certain stage. We studied the regulation during heterocyst differentiation of several essential components of the Anabaena cell division machinery and found that protein PatA, which is required for differentiation and is induced in differentiating cells, interacts with essential cell division factors and destabilizes the cell division complex. This suggests a mechanism for establishment of commitment to differentiation by inhibition of cell division.
format	article
author	Ana Valladares Cristina Velázquez-Suárez Antonia Herrero
author_facet	Ana Valladares Cristina Velázquez-Suárez Antonia Herrero
author_sort	Ana Valladares
title	Interactions of PatA with the Divisome during Heterocyst Differentiation in <italic toggle="yes">Anabaena</italic>
title_short	Interactions of PatA with the Divisome during Heterocyst Differentiation in <italic toggle="yes">Anabaena</italic>
title_full	Interactions of PatA with the Divisome during Heterocyst Differentiation in <italic toggle="yes">Anabaena</italic>
title_fullStr	Interactions of PatA with the Divisome during Heterocyst Differentiation in <italic toggle="yes">Anabaena</italic>
title_full_unstemmed	Interactions of PatA with the Divisome during Heterocyst Differentiation in <italic toggle="yes">Anabaena</italic>
title_sort	interactions of pata with the divisome during heterocyst differentiation in <italic toggle="yes">anabaena</italic>
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/f1c68bde44624ab29444b2a4e6e2cd43
work_keys_str_mv	AT anavalladares interactionsofpatawiththedivisomeduringheterocystdifferentiationinitalictoggleyesanabaenaitalic AT cristinavelazquezsuarez interactionsofpatawiththedivisomeduringheterocystdifferentiationinitalictoggleyesanabaenaitalic AT antoniaherrero interactionsofpatawiththedivisomeduringheterocystdifferentiationinitalictoggleyesanabaenaitalic
_version_	1718427924177092608

Interactions of PatA with the Divisome during Heterocyst Differentiation in <italic toggle="yes">Anabaena</italic>

Ejemplares similares