IFNγ-INDUCED KINURENINE PRODUCTION AND INDOLAMINE 2,3-DIOXYGENASE GENE EXPRESSION IN PSORIASIS

Abstract. An alternative pathway of L-tryptophan biotransformation is provided by the indolamine 2,3-dioxygenase (INDO), and it results into synthesis of kynurenine and other «distal» metabolites, playing a pivotal role in immunoregulation and down-regulation of immune inflammation. PBMCs from healt...

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Autores principales:	D. A. Kaut, R. S. Yamidanov, S. V. Sadovnikov, O. M. Kapuler, V. A. Vakhitov, S. V. Sibiryak
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2014
Materias:	ifnγ kinurenine indolamine 2,3-dioxygenase psoriasis Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/f1c87023d4414481bbd8cb99a6c90be0
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spelling	oai:doaj.org-article:f1c87023d4414481bbd8cb99a6c90be02021-11-18T08:03:39ZIFNγ-INDUCED KINURENINE PRODUCTION AND INDOLAMINE 2,3-DIOXYGENASE GENE EXPRESSION IN PSORIASIS1563-06252313-741X10.15789/1563-0625-2009-2-3-147-152https://doaj.org/article/f1c87023d4414481bbd8cb99a6c90be02014-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/277https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XAbstract. An alternative pathway of L-tryptophan biotransformation is provided by the indolamine 2,3-dioxygenase (INDO), and it results into synthesis of kynurenine and other «distal» metabolites, playing a pivotal role in immunoregulation and down-regulation of immune inflammation. PBMCs from healthy volunteers, patients with progressive vulgar psoriasis (PASI (M±SD) = 25.6±16.6), or patients with psoriatic arthropathy (PASI = 40.3±24.5). The cells were incubated for 24 hrs with IFNγ (500 IU/ml, stimulated cultures) or without cytokine (control cultures). Distinct abnormalities in spontaneous and induced kynurenine production (colorimetric method) and INDO expression (semi-quantitative RT-PCR) were observed in psoriasis and psoriatic arthritis. PBMCs from psoriatic patients, in comparison with healthy donors, were characterized with slightly increased spontaneous kynurenine production, spontaneous and IFNγ-induced INDO expression, while IFNγ-induced kynurenine levels were approximately two times higher. In psoriatic arthritis, spontaneous kynurenine production and INDO expression were significantly lower than in donor’s PBMC, whereas IFNγ-induced kynurenine production were the same as for the donors, while IFNγ – induced INDO expression was markedly increased.D. A. KautR. S. YamidanovS. V. SadovnikovO. M. KapulerV. A. VakhitovS. V. SibiryakSPb RAACIarticleifnγkinurenineindolamine 2,3-dioxygenasepsoriasisImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 11, Iss 2-3, Pp 147-152 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	ifnγ kinurenine indolamine 2,3-dioxygenase psoriasis Immunologic diseases. Allergy RC581-607
spellingShingle	ifnγ kinurenine indolamine 2,3-dioxygenase psoriasis Immunologic diseases. Allergy RC581-607 D. A. Kaut R. S. Yamidanov S. V. Sadovnikov O. M. Kapuler V. A. Vakhitov S. V. Sibiryak IFNγ-INDUCED KINURENINE PRODUCTION AND INDOLAMINE 2,3-DIOXYGENASE GENE EXPRESSION IN PSORIASIS
description	Abstract. An alternative pathway of L-tryptophan biotransformation is provided by the indolamine 2,3-dioxygenase (INDO), and it results into synthesis of kynurenine and other «distal» metabolites, playing a pivotal role in immunoregulation and down-regulation of immune inflammation. PBMCs from healthy volunteers, patients with progressive vulgar psoriasis (PASI (M±SD) = 25.6±16.6), or patients with psoriatic arthropathy (PASI = 40.3±24.5). The cells were incubated for 24 hrs with IFNγ (500 IU/ml, stimulated cultures) or without cytokine (control cultures). Distinct abnormalities in spontaneous and induced kynurenine production (colorimetric method) and INDO expression (semi-quantitative RT-PCR) were observed in psoriasis and psoriatic arthritis. PBMCs from psoriatic patients, in comparison with healthy donors, were characterized with slightly increased spontaneous kynurenine production, spontaneous and IFNγ-induced INDO expression, while IFNγ-induced kynurenine levels were approximately two times higher. In psoriatic arthritis, spontaneous kynurenine production and INDO expression were significantly lower than in donor’s PBMC, whereas IFNγ-induced kynurenine production were the same as for the donors, while IFNγ – induced INDO expression was markedly increased.
format	article
author	D. A. Kaut R. S. Yamidanov S. V. Sadovnikov O. M. Kapuler V. A. Vakhitov S. V. Sibiryak
author_facet	D. A. Kaut R. S. Yamidanov S. V. Sadovnikov O. M. Kapuler V. A. Vakhitov S. V. Sibiryak
author_sort	D. A. Kaut
title	IFNγ-INDUCED KINURENINE PRODUCTION AND INDOLAMINE 2,3-DIOXYGENASE GENE EXPRESSION IN PSORIASIS
title_short	IFNγ-INDUCED KINURENINE PRODUCTION AND INDOLAMINE 2,3-DIOXYGENASE GENE EXPRESSION IN PSORIASIS
title_full	IFNγ-INDUCED KINURENINE PRODUCTION AND INDOLAMINE 2,3-DIOXYGENASE GENE EXPRESSION IN PSORIASIS
title_fullStr	IFNγ-INDUCED KINURENINE PRODUCTION AND INDOLAMINE 2,3-DIOXYGENASE GENE EXPRESSION IN PSORIASIS
title_full_unstemmed	IFNγ-INDUCED KINURENINE PRODUCTION AND INDOLAMINE 2,3-DIOXYGENASE GENE EXPRESSION IN PSORIASIS
title_sort	ifnγ-induced kinurenine production and indolamine 2,3-dioxygenase gene expression in psoriasis
publisher	SPb RAACI
publishDate	2014
url	https://doaj.org/article/f1c87023d4414481bbd8cb99a6c90be0
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IFNγ-INDUCED KINURENINE PRODUCTION AND INDOLAMINE 2,3-DIOXYGENASE GENE EXPRESSION IN PSORIASIS

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