The DNA barcode of red fruit pandan (Pandanaceae) cultivar from Wamena, Papua Province, Indonesia based on matK gene

Abstract. Zebua LI, Gunaedi T, Budi IM, Lunga N. 2019. The DNA barcode of red fruit pandan (Pandanaceae) cultivar from Wamena, Papua Province, Indonesia based on matK gene. Biodiversitas 20: 3405-3412. The red fruit pandan has been used by the people of Wamena in central highlands of Papua as medici...

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Autores principales:	LISYE IRIANA ZEBUA, TRI GUNAEDI, I MADE BUDI, NELLY LUNGA
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2019
Materias:	dna barcode, genes matk, red fruit pandan, wamena Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/f1d7658a076b4b2fba3ad56b786136b2
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spelling	oai:doaj.org-article:f1d7658a076b4b2fba3ad56b786136b22021-11-21T21:57:23ZThe DNA barcode of red fruit pandan (Pandanaceae) cultivar from Wamena, Papua Province, Indonesia based on matK gene1412-033X2085-472210.13057/biodiv/d201138https://doaj.org/article/f1d7658a076b4b2fba3ad56b786136b22019-10-01T00:00:00Zhttps://smujo.id/biodiv/article/view/3891https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Zebua LI, Gunaedi T, Budi IM, Lunga N. 2019. The DNA barcode of red fruit pandan (Pandanaceae) cultivar from Wamena, Papua Province, Indonesia based on matK gene. Biodiversitas 20: 3405-3412. The red fruit pandan has been used by the people of Wamena in central highlands of Papua as medicinal plants, food ingredients, and religious ceremonies. Based on morphological characters, there are 39 cultivars of red fruit pandan in New Guinea. A standard method for plant species identification through DNA barcodes has been recommended to use matK gene. The research aimed to determine similarities in DNA barcode sequences of six red fruit pandan cultivars with its close relative that listed in NCBI system and to recommend the reliable DNA barcode for identification of this cultivar. Polymerase Chain Reaction was employed DNA to amplify matK gene fragments using an available forward primer (5’CGA TCT ATT CAT TCA ATA TTT C 3’) and reverse primer (5’TCT AGC ACA CGA AAGTCG AAG T 3’). The software BLAST, Bioedit, and ClustalX were used to analyze the data. Barcode DNA of red fruit pandan showed 1474 bp nucleotides sequence based on matK gene. An indication of six red fruit pandan cultivars showed that high similarity 99% with Pandanus conoideus Lam. It can be concluded that matK gene can be used to determine the species level in Pandanus.LISYE IRIANA ZEBUATRI GUNAEDII MADE BUDINELLY LUNGAMBI & UNS Soloarticledna barcode, genes matk, red fruit pandan, wamenaBiology (General)QH301-705.5ENBiodiversitas, Vol 20, Iss 11 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	dna barcode, genes matk, red fruit pandan, wamena Biology (General) QH301-705.5
spellingShingle	dna barcode, genes matk, red fruit pandan, wamena Biology (General) QH301-705.5 LISYE IRIANA ZEBUA TRI GUNAEDI I MADE BUDI NELLY LUNGA The DNA barcode of red fruit pandan (Pandanaceae) cultivar from Wamena, Papua Province, Indonesia based on matK gene
description	Abstract. Zebua LI, Gunaedi T, Budi IM, Lunga N. 2019. The DNA barcode of red fruit pandan (Pandanaceae) cultivar from Wamena, Papua Province, Indonesia based on matK gene. Biodiversitas 20: 3405-3412. The red fruit pandan has been used by the people of Wamena in central highlands of Papua as medicinal plants, food ingredients, and religious ceremonies. Based on morphological characters, there are 39 cultivars of red fruit pandan in New Guinea. A standard method for plant species identification through DNA barcodes has been recommended to use matK gene. The research aimed to determine similarities in DNA barcode sequences of six red fruit pandan cultivars with its close relative that listed in NCBI system and to recommend the reliable DNA barcode for identification of this cultivar. Polymerase Chain Reaction was employed DNA to amplify matK gene fragments using an available forward primer (5’CGA TCT ATT CAT TCA ATA TTT C 3’) and reverse primer (5’TCT AGC ACA CGA AAGTCG AAG T 3’). The software BLAST, Bioedit, and ClustalX were used to analyze the data. Barcode DNA of red fruit pandan showed 1474 bp nucleotides sequence based on matK gene. An indication of six red fruit pandan cultivars showed that high similarity 99% with Pandanus conoideus Lam. It can be concluded that matK gene can be used to determine the species level in Pandanus.
format	article
author	LISYE IRIANA ZEBUA TRI GUNAEDI I MADE BUDI NELLY LUNGA
author_facet	LISYE IRIANA ZEBUA TRI GUNAEDI I MADE BUDI NELLY LUNGA
author_sort	LISYE IRIANA ZEBUA
title	The DNA barcode of red fruit pandan (Pandanaceae) cultivar from Wamena, Papua Province, Indonesia based on matK gene
title_short	The DNA barcode of red fruit pandan (Pandanaceae) cultivar from Wamena, Papua Province, Indonesia based on matK gene
title_full	The DNA barcode of red fruit pandan (Pandanaceae) cultivar from Wamena, Papua Province, Indonesia based on matK gene
title_fullStr	The DNA barcode of red fruit pandan (Pandanaceae) cultivar from Wamena, Papua Province, Indonesia based on matK gene
title_full_unstemmed	The DNA barcode of red fruit pandan (Pandanaceae) cultivar from Wamena, Papua Province, Indonesia based on matK gene
title_sort	dna barcode of red fruit pandan (pandanaceae) cultivar from wamena, papua province, indonesia based on matk gene
publisher	MBI & UNS Solo
publishDate	2019
url	https://doaj.org/article/f1d7658a076b4b2fba3ad56b786136b2
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The DNA barcode of red fruit pandan (Pandanaceae) cultivar from Wamena, Papua Province, Indonesia based on matK gene

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