Verapamil induces autophagy to improve liver regeneration in non-alcoholic fatty liver mice

Verapamil can restore intracellular calcium homeostasis, increase the fusion of autophagosomes and lysosomes, reduce lipid droplet accumulation and inhibit inflammation and insulin resistance in high-fat-fed mice. The present study aimed to investigate verapamil's effect and its underlying live...

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Autores principales:	Jian-Lin Lai, Yuan-E Lian, Jun-Yi Wu, Yao-Dong Wang, Yan-Nan Bai
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	verapamil autophagy non-alcoholic fatty liver disease liver regeneration Diseases of the endocrine glands. Clinical endocrinology RC648-665 Cytology QH573-671 Physiology QP1-981
Acceso en línea:	https://doaj.org/article/f1d78d64a42b4635a9cced3b30dbec47
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spelling	oai:doaj.org-article:f1d78d64a42b4635a9cced3b30dbec472021-11-04T15:00:45ZVerapamil induces autophagy to improve liver regeneration in non-alcoholic fatty liver mice2162-39452162-397X10.1080/21623945.2021.1983241https://doaj.org/article/f1d78d64a42b4635a9cced3b30dbec472021-01-01T00:00:00Zhttp://dx.doi.org/10.1080/21623945.2021.1983241https://doaj.org/toc/2162-3945https://doaj.org/toc/2162-397XVerapamil can restore intracellular calcium homeostasis, increase the fusion of autophagosomes and lysosomes, reduce lipid droplet accumulation and inhibit inflammation and insulin resistance in high-fat-fed mice. The present study aimed to investigate verapamil's effect and its underlying liver regeneration mechanism in mice with non-alcoholic fatty liver. After 50% hepatectomy was performed, the changes of autophagy and liver regeneration were evaluated by detecting cell proliferation and autophagy at each time point. Then, 25mg/kg verapamil was injected intraperitoneally for 10 d before an operation in the mild to moderate fatty liver and severe fatty liver groups. The control group and mild to moderate fatty liver group reached the peak of proliferation at 24-48h after operation, and the mice with severe fatty liver and steatohepatitis reached the peak at 48-72h. Autophagy in the normal group and mild to moderate fatty liver group reached the peak 48 hours after operation. Verapamil injection can enhance autophagy, reduce the weight of fatty liver mice, improve liver function and liver regeneration. Verapamil can induce autophagy, improve hepatocyte function and promote hepatocyte regeneration through the mTOR independent signaling pathway, thus improving the process of liver regeneration after partial hepatectomy.Jian-Lin LaiYuan-E LianJun-Yi WuYao-Dong WangYan-Nan BaiTaylor & Francis Grouparticleverapamilautophagynon-alcoholic fatty liver diseaseliver regenerationDiseases of the endocrine glands. Clinical endocrinologyRC648-665CytologyQH573-671PhysiologyQP1-981ENAdipocyte, Vol 10, Iss 1, Pp 532-545 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	verapamil autophagy non-alcoholic fatty liver disease liver regeneration Diseases of the endocrine glands. Clinical endocrinology RC648-665 Cytology QH573-671 Physiology QP1-981
spellingShingle	verapamil autophagy non-alcoholic fatty liver disease liver regeneration Diseases of the endocrine glands. Clinical endocrinology RC648-665 Cytology QH573-671 Physiology QP1-981 Jian-Lin Lai Yuan-E Lian Jun-Yi Wu Yao-Dong Wang Yan-Nan Bai Verapamil induces autophagy to improve liver regeneration in non-alcoholic fatty liver mice
description	Verapamil can restore intracellular calcium homeostasis, increase the fusion of autophagosomes and lysosomes, reduce lipid droplet accumulation and inhibit inflammation and insulin resistance in high-fat-fed mice. The present study aimed to investigate verapamil's effect and its underlying liver regeneration mechanism in mice with non-alcoholic fatty liver. After 50% hepatectomy was performed, the changes of autophagy and liver regeneration were evaluated by detecting cell proliferation and autophagy at each time point. Then, 25mg/kg verapamil was injected intraperitoneally for 10 d before an operation in the mild to moderate fatty liver and severe fatty liver groups. The control group and mild to moderate fatty liver group reached the peak of proliferation at 24-48h after operation, and the mice with severe fatty liver and steatohepatitis reached the peak at 48-72h. Autophagy in the normal group and mild to moderate fatty liver group reached the peak 48 hours after operation. Verapamil injection can enhance autophagy, reduce the weight of fatty liver mice, improve liver function and liver regeneration. Verapamil can induce autophagy, improve hepatocyte function and promote hepatocyte regeneration through the mTOR independent signaling pathway, thus improving the process of liver regeneration after partial hepatectomy.
format	article
author	Jian-Lin Lai Yuan-E Lian Jun-Yi Wu Yao-Dong Wang Yan-Nan Bai
author_facet	Jian-Lin Lai Yuan-E Lian Jun-Yi Wu Yao-Dong Wang Yan-Nan Bai
author_sort	Jian-Lin Lai
title	Verapamil induces autophagy to improve liver regeneration in non-alcoholic fatty liver mice
title_short	Verapamil induces autophagy to improve liver regeneration in non-alcoholic fatty liver mice
title_full	Verapamil induces autophagy to improve liver regeneration in non-alcoholic fatty liver mice
title_fullStr	Verapamil induces autophagy to improve liver regeneration in non-alcoholic fatty liver mice
title_full_unstemmed	Verapamil induces autophagy to improve liver regeneration in non-alcoholic fatty liver mice
title_sort	verapamil induces autophagy to improve liver regeneration in non-alcoholic fatty liver mice
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/f1d78d64a42b4635a9cced3b30dbec47
work_keys_str_mv	AT jianlinlai verapamilinducesautophagytoimproveliverregenerationinnonalcoholicfattylivermice AT yuanelian verapamilinducesautophagytoimproveliverregenerationinnonalcoholicfattylivermice AT junyiwu verapamilinducesautophagytoimproveliverregenerationinnonalcoholicfattylivermice AT yaodongwang verapamilinducesautophagytoimproveliverregenerationinnonalcoholicfattylivermice AT yannanbai verapamilinducesautophagytoimproveliverregenerationinnonalcoholicfattylivermice
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Verapamil induces autophagy to improve liver regeneration in non-alcoholic fatty liver mice

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