Transcriptome of rhesus macaque (Macaca mulatta) exposed to total-body irradiation

Abstract The field of biodosimetry has seen a paradigm shift towards an increased use of molecular phenotyping technologies including omics and miRNA, in addition to conventional cytogenetic techniques. Here, we have used a nonhuman primate (NHP) model to study the impact of gamma-irradiation on alt...

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Autores principales:	Yaoxiang Li, Jatinder Singh, Rency Varghese, Yubo Zhang, Oluseyi O. Fatanmi, Amrita K. Cheema, Vijay K. Singh
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f1d90baa7a614db38a53c2f44c8b6a09
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spelling	oai:doaj.org-article:f1d90baa7a614db38a53c2f44c8b6a092021-12-02T13:17:55ZTranscriptome of rhesus macaque (Macaca mulatta) exposed to total-body irradiation10.1038/s41598-021-85669-62045-2322https://doaj.org/article/f1d90baa7a614db38a53c2f44c8b6a092021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85669-6https://doaj.org/toc/2045-2322Abstract The field of biodosimetry has seen a paradigm shift towards an increased use of molecular phenotyping technologies including omics and miRNA, in addition to conventional cytogenetic techniques. Here, we have used a nonhuman primate (NHP) model to study the impact of gamma-irradiation on alterations in blood-based gene expression. With a goal to delineate radiation induced changes in gene expression, we followed eight NHPs for 60 days after exposure to 6.5 Gy gamma-radiation for survival outcomes. Analysis of differential gene expression in response to radiation exposure yielded 26,944 dysregulated genes that were not significantly impacted by sex. Further analysis showed an increased association of several pathways including IL-3 signaling, ephrin receptor signaling, ErbB signaling, nitric oxide signaling in the cardiovascular system, Wnt/β-catenin signaling, and inflammasome pathway, which were associated with positive survival outcomes in NHPs after acute exposure to radiation. This study provides novel insights into major pathways and networks involved in radiation-induced injuries that may identify biomarkers for radiation injury.Yaoxiang LiJatinder SinghRency VargheseYubo ZhangOluseyi O. FatanmiAmrita K. CheemaVijay K. SinghNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yaoxiang Li Jatinder Singh Rency Varghese Yubo Zhang Oluseyi O. Fatanmi Amrita K. Cheema Vijay K. Singh Transcriptome of rhesus macaque (Macaca mulatta) exposed to total-body irradiation
description	Abstract The field of biodosimetry has seen a paradigm shift towards an increased use of molecular phenotyping technologies including omics and miRNA, in addition to conventional cytogenetic techniques. Here, we have used a nonhuman primate (NHP) model to study the impact of gamma-irradiation on alterations in blood-based gene expression. With a goal to delineate radiation induced changes in gene expression, we followed eight NHPs for 60 days after exposure to 6.5 Gy gamma-radiation for survival outcomes. Analysis of differential gene expression in response to radiation exposure yielded 26,944 dysregulated genes that were not significantly impacted by sex. Further analysis showed an increased association of several pathways including IL-3 signaling, ephrin receptor signaling, ErbB signaling, nitric oxide signaling in the cardiovascular system, Wnt/β-catenin signaling, and inflammasome pathway, which were associated with positive survival outcomes in NHPs after acute exposure to radiation. This study provides novel insights into major pathways and networks involved in radiation-induced injuries that may identify biomarkers for radiation injury.
format	article
author	Yaoxiang Li Jatinder Singh Rency Varghese Yubo Zhang Oluseyi O. Fatanmi Amrita K. Cheema Vijay K. Singh
author_facet	Yaoxiang Li Jatinder Singh Rency Varghese Yubo Zhang Oluseyi O. Fatanmi Amrita K. Cheema Vijay K. Singh
author_sort	Yaoxiang Li
title	Transcriptome of rhesus macaque (Macaca mulatta) exposed to total-body irradiation
title_short	Transcriptome of rhesus macaque (Macaca mulatta) exposed to total-body irradiation
title_full	Transcriptome of rhesus macaque (Macaca mulatta) exposed to total-body irradiation
title_fullStr	Transcriptome of rhesus macaque (Macaca mulatta) exposed to total-body irradiation
title_full_unstemmed	Transcriptome of rhesus macaque (Macaca mulatta) exposed to total-body irradiation
title_sort	transcriptome of rhesus macaque (macaca mulatta) exposed to total-body irradiation
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f1d90baa7a614db38a53c2f44c8b6a09
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Transcriptome of rhesus macaque (Macaca mulatta) exposed to total-body irradiation

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