A high throughput barley stripe mosaic virus vector for virus induced gene silencing in monocots and dicots.

Barley stripe mosaic virus (BSMV) is a single-stranded RNA virus with three genome components designated alpha, beta, and gamma. BSMV vectors have previously been shown to be efficient virus induced gene silencing (VIGS) vehicles in barley and wheat and have provided important information about host...

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Autores principales:	Cheng Yuan, Cui Li, Lijie Yan, Andrew O Jackson, Zhiyong Liu, Chenggui Han, Jialin Yu, Dawei Li
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f1db8498e8c745c3a25541b958ff6f57
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spelling	oai:doaj.org-article:f1db8498e8c745c3a25541b958ff6f572021-11-18T07:35:57ZA high throughput barley stripe mosaic virus vector for virus induced gene silencing in monocots and dicots.1932-620310.1371/journal.pone.0026468https://doaj.org/article/f1db8498e8c745c3a25541b958ff6f572011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22031834/?tool=EBIhttps://doaj.org/toc/1932-6203Barley stripe mosaic virus (BSMV) is a single-stranded RNA virus with three genome components designated alpha, beta, and gamma. BSMV vectors have previously been shown to be efficient virus induced gene silencing (VIGS) vehicles in barley and wheat and have provided important information about host genes functioning during pathogenesis as well as various aspects of genes functioning in development. To permit more effective use of BSMV VIGS for functional genomics experiments, we have developed an Agrobacterium delivery system for BSMV and have coupled this with a ligation independent cloning (LIC) strategy to mediate efficient cloning of host genes. Infiltrated Nicotiana benthamiana leaves provided excellent sources of virus for secondary BSMV infections and VIGS in cereals. The Agro/LIC BSMV VIGS vectors were able to function in high efficiency down regulation of phytoene desaturase (PDS), magnesium chelatase subunit H (ChlH), and plastid transketolase (TK) gene silencing in N. benthamiana and in the monocots, wheat, barley, and the model grass, Brachypodium distachyon. Suppression of an Arabidopsis orthologue cloned from wheat (TaPMR5) also interfered with wheat powdery mildew (Blumeria graminis f. sp. tritici) infections in a manner similar to that of the A. thaliana PMR5 loss-of-function allele. These results imply that the PMR5 gene has maintained similar functions across monocot and dicot families. Our BSMV VIGS system provides substantial advantages in expense, cloning efficiency, ease of manipulation and ability to apply VIGS for high throughput genomics studies.Cheng YuanCui LiLijie YanAndrew O JacksonZhiyong LiuChenggui HanJialin YuDawei LiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 10, p e26468 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Cheng Yuan Cui Li Lijie Yan Andrew O Jackson Zhiyong Liu Chenggui Han Jialin Yu Dawei Li A high throughput barley stripe mosaic virus vector for virus induced gene silencing in monocots and dicots.
description	Barley stripe mosaic virus (BSMV) is a single-stranded RNA virus with three genome components designated alpha, beta, and gamma. BSMV vectors have previously been shown to be efficient virus induced gene silencing (VIGS) vehicles in barley and wheat and have provided important information about host genes functioning during pathogenesis as well as various aspects of genes functioning in development. To permit more effective use of BSMV VIGS for functional genomics experiments, we have developed an Agrobacterium delivery system for BSMV and have coupled this with a ligation independent cloning (LIC) strategy to mediate efficient cloning of host genes. Infiltrated Nicotiana benthamiana leaves provided excellent sources of virus for secondary BSMV infections and VIGS in cereals. The Agro/LIC BSMV VIGS vectors were able to function in high efficiency down regulation of phytoene desaturase (PDS), magnesium chelatase subunit H (ChlH), and plastid transketolase (TK) gene silencing in N. benthamiana and in the monocots, wheat, barley, and the model grass, Brachypodium distachyon. Suppression of an Arabidopsis orthologue cloned from wheat (TaPMR5) also interfered with wheat powdery mildew (Blumeria graminis f. sp. tritici) infections in a manner similar to that of the A. thaliana PMR5 loss-of-function allele. These results imply that the PMR5 gene has maintained similar functions across monocot and dicot families. Our BSMV VIGS system provides substantial advantages in expense, cloning efficiency, ease of manipulation and ability to apply VIGS for high throughput genomics studies.
format	article
author	Cheng Yuan Cui Li Lijie Yan Andrew O Jackson Zhiyong Liu Chenggui Han Jialin Yu Dawei Li
author_facet	Cheng Yuan Cui Li Lijie Yan Andrew O Jackson Zhiyong Liu Chenggui Han Jialin Yu Dawei Li
author_sort	Cheng Yuan
title	A high throughput barley stripe mosaic virus vector for virus induced gene silencing in monocots and dicots.
title_short	A high throughput barley stripe mosaic virus vector for virus induced gene silencing in monocots and dicots.
title_full	A high throughput barley stripe mosaic virus vector for virus induced gene silencing in monocots and dicots.
title_fullStr	A high throughput barley stripe mosaic virus vector for virus induced gene silencing in monocots and dicots.
title_full_unstemmed	A high throughput barley stripe mosaic virus vector for virus induced gene silencing in monocots and dicots.
title_sort	high throughput barley stripe mosaic virus vector for virus induced gene silencing in monocots and dicots.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/f1db8498e8c745c3a25541b958ff6f57
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A high throughput barley stripe mosaic virus vector for virus induced gene silencing in monocots and dicots.

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