Environmental DNA detection of an invasive ant species (Linepithema humile) from soil samples

Abstract Alien ant species (Formicidae, Hymenoptera) cause serious damage worldwide. Early detection of invasion and rapid management are significant for controlling these species. However, these attempts are sometimes hindered by the need for direct detection techniques, such as capture, visual obs...

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Autores principales:	Tetsu Yasashimoto, Masayuki K. Sakata, Tomoya Sakita, Satoko Nakajima, Mamiko Ozaki, Toshifumi Minamoto
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f1dfb33dfd4047e8aab53eed932e564d
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spelling	oai:doaj.org-article:f1dfb33dfd4047e8aab53eed932e564d2021-12-02T14:47:31ZEnvironmental DNA detection of an invasive ant species (Linepithema humile) from soil samples10.1038/s41598-021-89993-92045-2322https://doaj.org/article/f1dfb33dfd4047e8aab53eed932e564d2021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89993-9https://doaj.org/toc/2045-2322Abstract Alien ant species (Formicidae, Hymenoptera) cause serious damage worldwide. Early detection of invasion and rapid management are significant for controlling these species. However, these attempts are sometimes hindered by the need for direct detection techniques, such as capture, visual observation, or morphological identification. In this study, we demonstrated that environmental DNA (eDNA) analysis can be used as a monitoring tool for alien ants using Linepithema humile (Argentine ant), one of the most invasive ants, as a model species. We designed a new real-time PCR assay specific to L. humile and successfully detected eDNA from the surface soil. The reliability of eDNA analysis was substantiated by comparing eDNA detection results with traditional survey results. Additionally, we examined the relationship between eDNA concentration and distance from nests and trails. Our results support the effectiveness of eDNA for alien ant monitoring and suggest that this new method could improve our ability to detect invasive ant species.Tetsu YasashimotoMasayuki K. SakataTomoya SakitaSatoko NakajimaMamiko OzakiToshifumi MinamotoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tetsu Yasashimoto Masayuki K. Sakata Tomoya Sakita Satoko Nakajima Mamiko Ozaki Toshifumi Minamoto Environmental DNA detection of an invasive ant species (Linepithema humile) from soil samples
description	Abstract Alien ant species (Formicidae, Hymenoptera) cause serious damage worldwide. Early detection of invasion and rapid management are significant for controlling these species. However, these attempts are sometimes hindered by the need for direct detection techniques, such as capture, visual observation, or morphological identification. In this study, we demonstrated that environmental DNA (eDNA) analysis can be used as a monitoring tool for alien ants using Linepithema humile (Argentine ant), one of the most invasive ants, as a model species. We designed a new real-time PCR assay specific to L. humile and successfully detected eDNA from the surface soil. The reliability of eDNA analysis was substantiated by comparing eDNA detection results with traditional survey results. Additionally, we examined the relationship between eDNA concentration and distance from nests and trails. Our results support the effectiveness of eDNA for alien ant monitoring and suggest that this new method could improve our ability to detect invasive ant species.
format	article
author	Tetsu Yasashimoto Masayuki K. Sakata Tomoya Sakita Satoko Nakajima Mamiko Ozaki Toshifumi Minamoto
author_facet	Tetsu Yasashimoto Masayuki K. Sakata Tomoya Sakita Satoko Nakajima Mamiko Ozaki Toshifumi Minamoto
author_sort	Tetsu Yasashimoto
title	Environmental DNA detection of an invasive ant species (Linepithema humile) from soil samples
title_short	Environmental DNA detection of an invasive ant species (Linepithema humile) from soil samples
title_full	Environmental DNA detection of an invasive ant species (Linepithema humile) from soil samples
title_fullStr	Environmental DNA detection of an invasive ant species (Linepithema humile) from soil samples
title_full_unstemmed	Environmental DNA detection of an invasive ant species (Linepithema humile) from soil samples
title_sort	environmental dna detection of an invasive ant species (linepithema humile) from soil samples
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f1dfb33dfd4047e8aab53eed932e564d
work_keys_str_mv	AT tetsuyasashimoto environmentaldnadetectionofaninvasiveantspecieslinepithemahumilefromsoilsamples AT masayukiksakata environmentaldnadetectionofaninvasiveantspecieslinepithemahumilefromsoilsamples AT tomoyasakita environmentaldnadetectionofaninvasiveantspecieslinepithemahumilefromsoilsamples AT satokonakajima environmentaldnadetectionofaninvasiveantspecieslinepithemahumilefromsoilsamples AT mamikoozaki environmentaldnadetectionofaninvasiveantspecieslinepithemahumilefromsoilsamples AT toshifumiminamoto environmentaldnadetectionofaninvasiveantspecieslinepithemahumilefromsoilsamples
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Environmental DNA detection of an invasive ant species (Linepithema humile) from soil samples

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