Effect of prolonged storage at 2°C–6°C for 120 h on the coagulation factors of thawed cryoprecipitate: Can we extend its shelf life post thaw beyond 4 h?

BACKGROUND: Cryoprecipitate helps in replenishing important coagulation factors like fibrinogen, Factor VIII and von Willebrand factor without running the risk of volume overload. It is very useful in the treatment of trauma patients with active bleeding and works best when administered early. Exten...

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Autores principales:	Arghyadeep Marik, Joseph Philip, Rajiv Singh Mallhi, Neerja Kushwaha, Sudeep Kumar, Amit Kumar Biswas, Arvind Kumar Yadav
Formato:	article
Lenguaje:	EN
Publicado:	Wolters Kluwer Medknow Publications 2021
Materias:	coagulation factors prolonged storage shelf life thawed cryoprecipitate Diseases of the blood and blood-forming organs RC633-647.5
Acceso en línea:	https://doaj.org/article/f1ea3c3a13744a93951a7c182c990119
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spelling	oai:doaj.org-article:f1ea3c3a13744a93951a7c182c9901192021-11-12T10:08:28ZEffect of prolonged storage at 2°C–6°C for 120 h on the coagulation factors of thawed cryoprecipitate: Can we extend its shelf life post thaw beyond 4 h?0973-62471998-356510.4103/ajts.AJTS_38_19https://doaj.org/article/f1ea3c3a13744a93951a7c182c9901192021-01-01T00:00:00Zhttp://www.ajts.org/article.asp?issn=0973-6247;year=2021;volume=15;issue=2;spage=146;epage=150;aulast=Marikhttps://doaj.org/toc/0973-6247https://doaj.org/toc/1998-3565BACKGROUND: Cryoprecipitate helps in replenishing important coagulation factors like fibrinogen, Factor VIII and von Willebrand factor without running the risk of volume overload. It is very useful in the treatment of trauma patients with active bleeding and works best when administered early. Extending the shelf life of thawed cryoprecipitate beyond 4 hours enables us to manage inventory better, reduces the burden of demand vs supply as well as minimizes wastage. It can also help in logistically supporting the transfusion services in making cryoprecipitate readily available in mass casualty scenarios (war, natural calamity) in remote locations by reducing the time required for thawing cryoprecipitate and the need for costly storage equipment. AIM: The aim of this study was to compare the levels of Factor VIII, Fibrinogen and von Willebrand factor on thawed cryoprecipitate after prolonged storage for 5 days at a temperature of 2-6°C.METHODOLOGY: The above mentioned coagulation factors were analyzed in cryoprecipitate at the time of product thaw and again after 120 hours of 2 to 6°C storage using fully automated coagulation analyser (STA Compact Max). All parameters were expressed as Mean ± Standard deviation and were analyzed using paired t-test with level of significance, P < 0.05.RESULTS: There was a significant decrease in the level of Factor VIII, whereas the levels of fibrinogen and von Willebrand Factor remained stable during the storage period. All the cryoprecipitate units retained factor activities above therapeutic range even after 5 days of storage at 2-6°C. CONCLUSION: Although the levels of clotting factors are reduced during storage, they are still maintained above the therapeutic range. In scenarios where maintaining frozen cryoprecipitate inventory is a logistical challenge and emergency massive demands of cryoprecipitate are foreseen, the use of pre-thawed cryoprecipitate can be considered as a viable option.Arghyadeep MarikJoseph PhilipRajiv Singh MallhiNeerja KushwahaSudeep KumarAmit Kumar BiswasArvind Kumar YadavWolters Kluwer Medknow Publicationsarticlecoagulation factorsprolonged storageshelf lifethawed cryoprecipitateDiseases of the blood and blood-forming organsRC633-647.5ENAsian Journal of Transfusion Science, Vol 15, Iss 2, Pp 146-150 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	coagulation factors prolonged storage shelf life thawed cryoprecipitate Diseases of the blood and blood-forming organs RC633-647.5
spellingShingle	coagulation factors prolonged storage shelf life thawed cryoprecipitate Diseases of the blood and blood-forming organs RC633-647.5 Arghyadeep Marik Joseph Philip Rajiv Singh Mallhi Neerja Kushwaha Sudeep Kumar Amit Kumar Biswas Arvind Kumar Yadav Effect of prolonged storage at 2°C–6°C for 120 h on the coagulation factors of thawed cryoprecipitate: Can we extend its shelf life post thaw beyond 4 h?
description	BACKGROUND: Cryoprecipitate helps in replenishing important coagulation factors like fibrinogen, Factor VIII and von Willebrand factor without running the risk of volume overload. It is very useful in the treatment of trauma patients with active bleeding and works best when administered early. Extending the shelf life of thawed cryoprecipitate beyond 4 hours enables us to manage inventory better, reduces the burden of demand vs supply as well as minimizes wastage. It can also help in logistically supporting the transfusion services in making cryoprecipitate readily available in mass casualty scenarios (war, natural calamity) in remote locations by reducing the time required for thawing cryoprecipitate and the need for costly storage equipment. AIM: The aim of this study was to compare the levels of Factor VIII, Fibrinogen and von Willebrand factor on thawed cryoprecipitate after prolonged storage for 5 days at a temperature of 2-6°C.METHODOLOGY: The above mentioned coagulation factors were analyzed in cryoprecipitate at the time of product thaw and again after 120 hours of 2 to 6°C storage using fully automated coagulation analyser (STA Compact Max). All parameters were expressed as Mean ± Standard deviation and were analyzed using paired t-test with level of significance, P < 0.05.RESULTS: There was a significant decrease in the level of Factor VIII, whereas the levels of fibrinogen and von Willebrand Factor remained stable during the storage period. All the cryoprecipitate units retained factor activities above therapeutic range even after 5 days of storage at 2-6°C. CONCLUSION: Although the levels of clotting factors are reduced during storage, they are still maintained above the therapeutic range. In scenarios where maintaining frozen cryoprecipitate inventory is a logistical challenge and emergency massive demands of cryoprecipitate are foreseen, the use of pre-thawed cryoprecipitate can be considered as a viable option.
format	article
author	Arghyadeep Marik Joseph Philip Rajiv Singh Mallhi Neerja Kushwaha Sudeep Kumar Amit Kumar Biswas Arvind Kumar Yadav
author_facet	Arghyadeep Marik Joseph Philip Rajiv Singh Mallhi Neerja Kushwaha Sudeep Kumar Amit Kumar Biswas Arvind Kumar Yadav
author_sort	Arghyadeep Marik
title	Effect of prolonged storage at 2°C–6°C for 120 h on the coagulation factors of thawed cryoprecipitate: Can we extend its shelf life post thaw beyond 4 h?
title_short	Effect of prolonged storage at 2°C–6°C for 120 h on the coagulation factors of thawed cryoprecipitate: Can we extend its shelf life post thaw beyond 4 h?
title_full	Effect of prolonged storage at 2°C–6°C for 120 h on the coagulation factors of thawed cryoprecipitate: Can we extend its shelf life post thaw beyond 4 h?
title_fullStr	Effect of prolonged storage at 2°C–6°C for 120 h on the coagulation factors of thawed cryoprecipitate: Can we extend its shelf life post thaw beyond 4 h?
title_full_unstemmed	Effect of prolonged storage at 2°C–6°C for 120 h on the coagulation factors of thawed cryoprecipitate: Can we extend its shelf life post thaw beyond 4 h?
title_sort	effect of prolonged storage at 2°c–6°c for 120 h on the coagulation factors of thawed cryoprecipitate: can we extend its shelf life post thaw beyond 4 h?
publisher	Wolters Kluwer Medknow Publications
publishDate	2021
url	https://doaj.org/article/f1ea3c3a13744a93951a7c182c990119
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Effect of prolonged storage at 2°C–6°C for 120 h on the coagulation factors of thawed cryoprecipitate: Can we extend its shelf life post thaw beyond 4 h?

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