Bone marrow p16INK4a-deficiency does not modulate obesity, glucose homeostasis or atherosclerosis development.
<h4>Objective</h4>A genomic region near the CDKN2A locus, encoding p16(INK4a), has been associated to type 2 diabetes and atherosclerotic vascular disease, conditions in which inflammation plays an important role. Recently, we found that deficiency of p16(INK4a) results in decreased infl...
Guardado en:
| Autores principales: | , , , , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Public Library of Science (PLoS)
2012
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/f1eb7ebaa8ee485b9bc67339bc01578d |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:f1eb7ebaa8ee485b9bc67339bc01578d |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:f1eb7ebaa8ee485b9bc67339bc01578d2021-11-18T07:26:07ZBone marrow p16INK4a-deficiency does not modulate obesity, glucose homeostasis or atherosclerosis development.1932-620310.1371/journal.pone.0032440https://doaj.org/article/f1eb7ebaa8ee485b9bc67339bc01578d2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22403661/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Objective</h4>A genomic region near the CDKN2A locus, encoding p16(INK4a), has been associated to type 2 diabetes and atherosclerotic vascular disease, conditions in which inflammation plays an important role. Recently, we found that deficiency of p16(INK4a) results in decreased inflammatory signaling in murine macrophages and that p16(INK4a) influences the phenotype of human adipose tissue macrophages. Therefore, we investigated the influence of immune cell p16(INK4a) on glucose tolerance and atherosclerosis in mice.<h4>Methods and results</h4>Bone marrow p16(INK4a)-deficiency in C57Bl6 mice did not influence high fat diet-induced obesity nor plasma glucose and lipid levels. Glucose tolerance tests showed no alterations in high fat diet-induced glucose intolerance. While bone marrow p16(INK4a)-deficiency did not affect the gene expression profile of adipose tissue, hepatic expression of the alternative markers Chi3l3, Mgl2 and IL10 was increased and the induction of pro-inflammatory Nos2 was restrained on the high fat diet. Bone marrow p16(INK4a)-deficiency in low density lipoprotein receptor-deficient mice did not affect western diet-induced atherosclerotic plaque size or morphology. In line, plasma lipid levels remained unaffected and p16(INK4a)-deficient macrophages displayed equal cholesterol uptake and efflux compared to wild type macrophages.<h4>Conclusion</h4>Bone marrow p16(INK4a)-deficiency does not affect plasma lipids, obesity, glucose tolerance or atherosclerosis in mice.Kristiaan WoutersCéline CudejkoMarion J J GijbelsLucia FuentesKadiombo BantubungiJonathan VanhoutteRebecca DièvartCharlotte PaquetEmmanuel BouchaertSarah Anissa HannouFlorence GizardAnne TailleuxMenno P J de WintherBart StaelsRéjane PaumellePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 3, p e32440 (2012) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Kristiaan Wouters Céline Cudejko Marion J J Gijbels Lucia Fuentes Kadiombo Bantubungi Jonathan Vanhoutte Rebecca Dièvart Charlotte Paquet Emmanuel Bouchaert Sarah Anissa Hannou Florence Gizard Anne Tailleux Menno P J de Winther Bart Staels Réjane Paumelle Bone marrow p16INK4a-deficiency does not modulate obesity, glucose homeostasis or atherosclerosis development. |
| description |
<h4>Objective</h4>A genomic region near the CDKN2A locus, encoding p16(INK4a), has been associated to type 2 diabetes and atherosclerotic vascular disease, conditions in which inflammation plays an important role. Recently, we found that deficiency of p16(INK4a) results in decreased inflammatory signaling in murine macrophages and that p16(INK4a) influences the phenotype of human adipose tissue macrophages. Therefore, we investigated the influence of immune cell p16(INK4a) on glucose tolerance and atherosclerosis in mice.<h4>Methods and results</h4>Bone marrow p16(INK4a)-deficiency in C57Bl6 mice did not influence high fat diet-induced obesity nor plasma glucose and lipid levels. Glucose tolerance tests showed no alterations in high fat diet-induced glucose intolerance. While bone marrow p16(INK4a)-deficiency did not affect the gene expression profile of adipose tissue, hepatic expression of the alternative markers Chi3l3, Mgl2 and IL10 was increased and the induction of pro-inflammatory Nos2 was restrained on the high fat diet. Bone marrow p16(INK4a)-deficiency in low density lipoprotein receptor-deficient mice did not affect western diet-induced atherosclerotic plaque size or morphology. In line, plasma lipid levels remained unaffected and p16(INK4a)-deficient macrophages displayed equal cholesterol uptake and efflux compared to wild type macrophages.<h4>Conclusion</h4>Bone marrow p16(INK4a)-deficiency does not affect plasma lipids, obesity, glucose tolerance or atherosclerosis in mice. |
| format |
article |
| author |
Kristiaan Wouters Céline Cudejko Marion J J Gijbels Lucia Fuentes Kadiombo Bantubungi Jonathan Vanhoutte Rebecca Dièvart Charlotte Paquet Emmanuel Bouchaert Sarah Anissa Hannou Florence Gizard Anne Tailleux Menno P J de Winther Bart Staels Réjane Paumelle |
| author_facet |
Kristiaan Wouters Céline Cudejko Marion J J Gijbels Lucia Fuentes Kadiombo Bantubungi Jonathan Vanhoutte Rebecca Dièvart Charlotte Paquet Emmanuel Bouchaert Sarah Anissa Hannou Florence Gizard Anne Tailleux Menno P J de Winther Bart Staels Réjane Paumelle |
| author_sort |
Kristiaan Wouters |
| title |
Bone marrow p16INK4a-deficiency does not modulate obesity, glucose homeostasis or atherosclerosis development. |
| title_short |
Bone marrow p16INK4a-deficiency does not modulate obesity, glucose homeostasis or atherosclerosis development. |
| title_full |
Bone marrow p16INK4a-deficiency does not modulate obesity, glucose homeostasis or atherosclerosis development. |
| title_fullStr |
Bone marrow p16INK4a-deficiency does not modulate obesity, glucose homeostasis or atherosclerosis development. |
| title_full_unstemmed |
Bone marrow p16INK4a-deficiency does not modulate obesity, glucose homeostasis or atherosclerosis development. |
| title_sort |
bone marrow p16ink4a-deficiency does not modulate obesity, glucose homeostasis or atherosclerosis development. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2012 |
| url |
https://doaj.org/article/f1eb7ebaa8ee485b9bc67339bc01578d |
| work_keys_str_mv |
AT kristiaanwouters bonemarrowp16ink4adeficiencydoesnotmodulateobesityglucosehomeostasisoratherosclerosisdevelopment AT celinecudejko bonemarrowp16ink4adeficiencydoesnotmodulateobesityglucosehomeostasisoratherosclerosisdevelopment AT marionjjgijbels bonemarrowp16ink4adeficiencydoesnotmodulateobesityglucosehomeostasisoratherosclerosisdevelopment AT luciafuentes bonemarrowp16ink4adeficiencydoesnotmodulateobesityglucosehomeostasisoratherosclerosisdevelopment AT kadiombobantubungi bonemarrowp16ink4adeficiencydoesnotmodulateobesityglucosehomeostasisoratherosclerosisdevelopment AT jonathanvanhoutte bonemarrowp16ink4adeficiencydoesnotmodulateobesityglucosehomeostasisoratherosclerosisdevelopment AT rebeccadievart bonemarrowp16ink4adeficiencydoesnotmodulateobesityglucosehomeostasisoratherosclerosisdevelopment AT charlottepaquet bonemarrowp16ink4adeficiencydoesnotmodulateobesityglucosehomeostasisoratherosclerosisdevelopment AT emmanuelbouchaert bonemarrowp16ink4adeficiencydoesnotmodulateobesityglucosehomeostasisoratherosclerosisdevelopment AT sarahanissahannou bonemarrowp16ink4adeficiencydoesnotmodulateobesityglucosehomeostasisoratherosclerosisdevelopment AT florencegizard bonemarrowp16ink4adeficiencydoesnotmodulateobesityglucosehomeostasisoratherosclerosisdevelopment AT annetailleux bonemarrowp16ink4adeficiencydoesnotmodulateobesityglucosehomeostasisoratherosclerosisdevelopment AT mennopjdewinther bonemarrowp16ink4adeficiencydoesnotmodulateobesityglucosehomeostasisoratherosclerosisdevelopment AT bartstaels bonemarrowp16ink4adeficiencydoesnotmodulateobesityglucosehomeostasisoratherosclerosisdevelopment AT rejanepaumelle bonemarrowp16ink4adeficiencydoesnotmodulateobesityglucosehomeostasisoratherosclerosisdevelopment |
| _version_ |
1718423508384481280 |