ATP signaling in the integrative neural center of Aplysia californica

Abstract ATP and its ionotropic P2X receptors are components of the most ancient signaling system. However, little is known about the distribution and function of purinergic transmission in invertebrates. Here, we cloned, expressed, and pharmacologically characterized the P2X receptors in the sea sl...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	János Györi, Andrea B. Kohn, Daria Y. Romanova, Leonid L. Moroz
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f1eddd3a92274b66b13770dca93dfa10
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f1eddd3a92274b66b13770dca93dfa10
record_format	dspace
spelling	oai:doaj.org-article:f1eddd3a92274b66b13770dca93dfa102021-12-02T13:34:32ZATP signaling in the integrative neural center of Aplysia californica10.1038/s41598-021-84981-52045-2322https://doaj.org/article/f1eddd3a92274b66b13770dca93dfa102021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84981-5https://doaj.org/toc/2045-2322Abstract ATP and its ionotropic P2X receptors are components of the most ancient signaling system. However, little is known about the distribution and function of purinergic transmission in invertebrates. Here, we cloned, expressed, and pharmacologically characterized the P2X receptors in the sea slug Aplysia californica—a prominent neuroscience model. AcP2X receptors were successfully expressed in Xenopus oocytes and displayed activation by ATP with two-phased kinetics and Na+-dependence. Pharmacologically, they were different from other P2X receptors. The ATP analog, Bz-ATP, was a less effective agonist than ATP, and PPADS was a more potent inhibitor of the AcP2X receptors than the suramin. AcP2X were uniquely expressed within the cerebral F-cluster, the multifunctional integrative neurosecretory center. AcP2X receptors were also detected in the chemosensory structures and the early cleavage stages. Therefore, in molluscs, rapid ATP-dependent signaling can be implicated both in development and diverse homeostatic functions. Furthermore, this study illuminates novel cellular and systemic features of P2X-type ligand-gated ion channels for deciphering the evolution of neurotransmitters.János GyöriAndrea B. KohnDaria Y. RomanovaLeonid L. MorozNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q János Györi Andrea B. Kohn Daria Y. Romanova Leonid L. Moroz ATP signaling in the integrative neural center of Aplysia californica
description	Abstract ATP and its ionotropic P2X receptors are components of the most ancient signaling system. However, little is known about the distribution and function of purinergic transmission in invertebrates. Here, we cloned, expressed, and pharmacologically characterized the P2X receptors in the sea slug Aplysia californica—a prominent neuroscience model. AcP2X receptors were successfully expressed in Xenopus oocytes and displayed activation by ATP with two-phased kinetics and Na+-dependence. Pharmacologically, they were different from other P2X receptors. The ATP analog, Bz-ATP, was a less effective agonist than ATP, and PPADS was a more potent inhibitor of the AcP2X receptors than the suramin. AcP2X were uniquely expressed within the cerebral F-cluster, the multifunctional integrative neurosecretory center. AcP2X receptors were also detected in the chemosensory structures and the early cleavage stages. Therefore, in molluscs, rapid ATP-dependent signaling can be implicated both in development and diverse homeostatic functions. Furthermore, this study illuminates novel cellular and systemic features of P2X-type ligand-gated ion channels for deciphering the evolution of neurotransmitters.
format	article
author	János Györi Andrea B. Kohn Daria Y. Romanova Leonid L. Moroz
author_facet	János Györi Andrea B. Kohn Daria Y. Romanova Leonid L. Moroz
author_sort	János Györi
title	ATP signaling in the integrative neural center of Aplysia californica
title_short	ATP signaling in the integrative neural center of Aplysia californica
title_full	ATP signaling in the integrative neural center of Aplysia californica
title_fullStr	ATP signaling in the integrative neural center of Aplysia californica
title_full_unstemmed	ATP signaling in the integrative neural center of Aplysia californica
title_sort	atp signaling in the integrative neural center of aplysia californica
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f1eddd3a92274b66b13770dca93dfa10
work_keys_str_mv	AT janosgyori atpsignalingintheintegrativeneuralcenterofaplysiacalifornica AT andreabkohn atpsignalingintheintegrativeneuralcenterofaplysiacalifornica AT dariayromanova atpsignalingintheintegrativeneuralcenterofaplysiacalifornica AT leonidlmoroz atpsignalingintheintegrativeneuralcenterofaplysiacalifornica
_version_	1718392770869067776

ATP signaling in the integrative neural center of Aplysia californica

Ejemplares similares