A Cas-embedding strategy for minimizing off-target effects of DNA base editors

A Cas-embedding strategy for minimizing off-target effects of DNA base editors

DNA base editors can display off-target effects on DNA and RNA. Here the authors embed the base editing enzymes in the middle of nCas9 to reduce these without impacting on-target editing.

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Autores principales: Yajing Liu, Changyang Zhou, Shisheng Huang, Lu Dang, Yu Wei, Jun He, Yingsi Zhou, Shaoshuai Mao, Wanyu Tao, Yu Zhang, Hui Yang, Xingxu Huang, Tian Chi
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2020
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Q
Acceso en línea:https://doaj.org/article/f1fdbef8038d4a33ade4dc6a223c19e3
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spelling oai:doaj.org-article:f1fdbef8038d4a33ade4dc6a223c19e32021-12-02T15:37:18ZA Cas-embedding strategy for minimizing off-target effects of DNA base editors10.1038/s41467-020-19690-02041-1723https://doaj.org/article/f1fdbef8038d4a33ade4dc6a223c19e32020-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-19690-0https://doaj.org/toc/2041-1723DNA base editors can display off-target effects on DNA and RNA. Here the authors embed the base editing enzymes in the middle of nCas9 to reduce these without impacting on-target editing.Yajing LiuChangyang ZhouShisheng HuangLu DangYu WeiJun HeYingsi ZhouShaoshuai MaoWanyu TaoYu ZhangHui YangXingxu HuangTian ChiNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-9 (2020)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Yajing Liu
Changyang Zhou
Shisheng Huang
Lu Dang
Yu Wei
Jun He
Yingsi Zhou
Shaoshuai Mao
Wanyu Tao
Yu Zhang
Hui Yang
Xingxu Huang
Tian Chi
A Cas-embedding strategy for minimizing off-target effects of DNA base editors
description DNA base editors can display off-target effects on DNA and RNA. Here the authors embed the base editing enzymes in the middle of nCas9 to reduce these without impacting on-target editing.
format article
author Yajing Liu
Changyang Zhou
Shisheng Huang
Lu Dang
Yu Wei
Jun He
Yingsi Zhou
Shaoshuai Mao
Wanyu Tao
Yu Zhang
Hui Yang
Xingxu Huang
Tian Chi
author_facet Yajing Liu
Changyang Zhou
Shisheng Huang
Lu Dang
Yu Wei
Jun He
Yingsi Zhou
Shaoshuai Mao
Wanyu Tao
Yu Zhang
Hui Yang
Xingxu Huang
Tian Chi
author_sort Yajing Liu
title A Cas-embedding strategy for minimizing off-target effects of DNA base editors
title_short A Cas-embedding strategy for minimizing off-target effects of DNA base editors
title_full A Cas-embedding strategy for minimizing off-target effects of DNA base editors
title_fullStr A Cas-embedding strategy for minimizing off-target effects of DNA base editors
title_full_unstemmed A Cas-embedding strategy for minimizing off-target effects of DNA base editors
title_sort cas-embedding strategy for minimizing off-target effects of dna base editors
publisher Nature Portfolio
publishDate 2020
url https://doaj.org/article/f1fdbef8038d4a33ade4dc6a223c19e3
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